Supp. file 4. Alignment of all Macrobiotus hufelandi group sequences for the 28S gene. 
https://doi.org/10.5852/ejt.2021.758.1429.4631

>FJ435751_Macrobiotus_vladimiri_Tar221_Spain_28S
NNNNNNNNNNNNNNNNNNNCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGATGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCA-GTCTCCNNNNNNNNNNNNNN
>FJ435752_Macrobiotus_vladimiri_Tar10_Spain_28S
NNNNNNNNNNNNNNNNNNNCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGATGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCA-GTCTCCNNNNNNNNNNNNNN
>FJ435754_Macrobiotus_vladimiri_Tar71_Spain_28S
NNNNNNNNNNNNNNNNNNNCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGATGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCA-GTCTCCNNNNNNNNNNNNNN
>FJ435755_Macrobiotus_vladimiri_Tar196_Spain_28S
NNNNNNNNNNNNNNNNNNNCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGATGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCA-GTCTCCNNNNNNNNNNNNNN
>C4218_V4_28S
NNNNNNNNNNNNNNNNAGTCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGGTGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCAGGTCTCCAAGNNNNNNNNNNN
>C4218_T5_28S
CTTTAAATGGGTGAGAAGTCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGGTGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTAACAGCNNN
>C4218_T6_28S
NNNNNNNNNNNNNAGAAGTCTGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGGTGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTANNNNNNNN
>C4218_V7_28S
NNNNNNNNNNNNNNNNNNNNNGGCTTGCTTAAATGCATTAGCTGAAGTCTGGACGTTGGATACGAGCGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCGAACGCTGAGTTATGGCGCCCGACGAGACGCTCATGAGATCCCAGAAAAGGTGTTGGTTGCTATAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCTGGCCTATACTCGGCCGTTGCAGCAGCAGCAACGATAAGTCAAGCTGCGACGAGTAGGAGGGTCGCGGCGGTGGCGTCGAAGGCTTACGGGTGTGAACCTGGCTGGAGCCGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAGCGAGAACCTTGAGGACTGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGATCCTAAGGTATCGGCGAAAGCCGTTCAGAAGCGGTGTGGATGTGCTTCACAGCACTTGTTACCTTGGTGAGCAATCAACTTGGTGATTTTACTGCACCTACCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGCATCGGATATTGGTCTGCTTTCGGGTAGACACATGCGGTAACGCAAATGAACTCGGAGACGCTGACACGTGCCCTAGAAAGAGTTGTCTTTTCTTCATAAGGCACAGGCTCCCTGGAATCGGATCATCCGGCGATAGGGAGATGGTGTCCGTAGAGCACCGCGGTTCTTGCGGTGTCTGGTGCGCACGTGTCGGTCCTTGAAAATCCGAGGGAGAGTGTGATTTTCGTGCCGGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTAACAGCNNN