The identification of the species of the ‘Spilogona contractifrons species-group’ and the ‘Spilogona nitidicauda species-group’ (Diptera, Muscidae) based on morphological and molecular analysis

  • Vera S. Sorokina Siberian Zoological Museum, Institute of Systematics and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Frunze str. 11, Novosibirsk 630091
  • Elena V. Shaikevich Laboratory of Insect Genetics, N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991
DOI: https://doi.org/10.5852/ejt.2018.484
Keywords: COI gene, DNA barcoding, flies, identification key, male terminalia

Abstract

Muscid species of the ‘Spilogona contractifrons species-group’ (Spilogona alticola (Malloch, 1920), S. arctica (Zetterstedt, 1838), S. contractifrons (Zetterstedt, 1838), S. orthosurstyla Xue & Tian, 1988) and of the ‘Spilogona nitidicauda species-group’ (S. nitidicauda (Schnabl, 1911), S. hissarensis Hennig, 1959, S. imitatrix (Malloch, 1921), S. platyfrons Sorokina, 2018) are notoriously difficult to distinguish. In this paper, their morphological features are analysed, images of the male head, frons and abdomen of all the species are given, and the male terminalia are figured. The study of extensive material has shown that all the morphologically recognised species in each of these groups are valid species. An identification key is provided for both groups of species. To confirm the morphological differences, genetic differences in the cytochrome oxidase I gene of flies of the ‘Spilogona contractifrons species-group’ and of the ‘Spilogona nitidicauda species-group’ were analysed. It is shown that members of both groups of species have not only distinguishing morphological characters but also fixed substitutions in the DNA sequences. Since a low interspecific polymorphism is known in the Muscidae Latreille, 1802, the revealed genetic distances confirm the existence of separate species or subspecies in each of the groups studied.

References

Collin J.E. 1930. A revision of the Greenland species of the anthomyid genus Limnophora sens. lat. (Diptera), with figures of the male genitalia of these and many other Palaearctic species. Transactions of the Entomological Society of London 78: 255–281. https://doi.org/10.1111/j.1365-2311.1930.tb00387.x

Gregor F., Rozkošný R., Barták M. & Vaňhara J. 2002. The Muscidae (Diptera) of Central Europe. Folia Facultatis Scientiarum Naturalium Universitatis Masarykianae Brunensis, Biologia 107: 1–280.

Haseyama K.L.F., Wiegmann B.M., Almeida E.A.B. & de Carvalho C.J.B. 2015. Say goodbye to tribes in the new house fly classification: a new molecular phylogenetic analysis and an updated biogeographical narrative for the Muscidae (Diptera). Molecular Phylogenetics and Evolution 89: 1–12. https://doi.org/10.1016/j.ympev.2015.04.006

Hennig W. 1959a. Muscidae [Part, Lieferung 204]. In: Lindner E. (ed.) Die Fliegen der palaearktischen Region 63b: 233–288. Schweizerbart, Stuttgart.

Hennig W. 1959b. Muscidae [Part, Lieferung 205]. In: Lindner E. (ed.) Die Fliegen der palaearktischen Region 63b: 289–336. Schweizerbart, Stuttgart.

Hennig W. 1959c. Muscidae [Part, Lieferung 207]. In: Lindner E. (ed.) Die Fliegen der palaearktischen Region 63b: 337–384. Schweizerbart, Stuttgart.

Huckett H.C. 1965. The Muscidae of Northern Canada, Alaska and Greenland (Diptera). Memoirs of the Entomological Society of Canada 42: 5–369. https://doi.org/10.4039/entm9742fv

Kutty S.N., Pape T., Pont A.C., Wiegmann B.M. & Meier R. 2008. The Muscoidea (Diptera: Calyptratae) are paraphyletic: evidence from four mitochondrial and four nuclear genes. Molecular Phylogenetics and Evolution 49: 639–652. https://doi.org/10.1016/j.ympev.2008.08.012

Kutty S.N., Pont A.C., Meier R. & Pape T. 2014. Complete tribal sampling reveals basal split in Muscidae (Diptera), confirms saprophagy as ancestral feeding mode, and reveals an evolutionary correlation between instar numbers and carnivory. Molecular Phylogenetics and Evolution 78: 349–364. https://doi.org/10.1016/j.ympev.2014.05.027

Michelsen V. 2006. Annotated catalogue of the Anthomyiidae, Fanniidae, Muscidae and Scathophagidae (Diptera: Muscoidea) of Greenland. Steenstrupia 29: 105–126.

Pont A.C. 1986. Family Muscidae. In: Soós Á. & Papp L. (eds) Catalogue of Palaearctic Diptera 11: 57–215. Akadémiai Kiadó, Budapest.

Ratnasingham S. & Hebert P.D.N. 2007. BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes 7: 355–364. https://doi.org/10.1111/j.1471-8286.2006.01678.x

Renaud A.K., Savage J. & Adamowicz S.J. 2012. DNA barcoding of Northern Nearctic Muscidae (Diptera) reveals high correspondence between morphological and molecular species limits. BMC Ecology 12 (1): 1–15. https://doi.org/10.1186/1472-6785-12-24

Savage J., Wheeler T.A. & Wiegmann B.M. 2004. Phylogenetic analysis of the genus Thricops Rondani (Diptera: Muscidae) based on molecular and morphological characters. Systematic Entomology 29: 395–414. https://doi.org/10.1111/j.0307-6970.2004.00252.x

Schuehli G.S.E., de Carvalho C.J.B. & Wiegmann B.M. 2004. Regarding the taxonomic status of Ophyra Robineau-Desvoidy (Diptera: Muscidae): a molecular approach. Zootaxa 712: 1–12.

Schuehli G.S.E, de Carvalho C.J.B. & Wiegmann BM. 2007. Molecular phylogenetics of the Muscidae (Diptera: Calyptratae): new ideas in a congruence context. Invertebrate Systematics 21: 263–278. https://doi.org/10.1071/IS06026

Shaikevich E.V. 2007. PCR-RFLP of the COI gene reliably differentiates Cx. pipiens, Cx. pipiens form molestus and Cx. torrentium of the pipiens Complex. European Mosquito Bulletin 23: 25–30.

Simon C., Frati F., Beckenbach A., Crespi B., Liu H. & Flook P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87 (6): 651–701. https://doi.org/10.1093/aesa/87.6.651

Sorokina V.S. 2012a. Fauna of Muscidae (Diptera) of the Altai Mountains. Proceedings of the Russian Entomological Society 83 (1): 193–222. [In Russian, with English abstract.]

Sorokina V.S. 2012b. The Muscidae (Diptera) of the Russian tundra zones. Report 1. Caucasian Entomological Bulletin 8 (2): 328–332. [In Russian, with English abstract.]

Sorokina V.S. 2013a. Fauno-genetic connections of Muscoidea (Diptera) of the high altitude of the Altai Mts and the latitudinal tundra of Taymyr Peninsula. In: Babin V.G., Tabakaev U.V., Dolgovykh S.V., Yurkova N.A., Aleynikova V.N. & Bondarenko A.V., Biodiversity, ecological issues of Gorny Altai and its neighboring regions: present, past and future: 115–118. Russia, Gorno-Altaisk. [In Russian, with English summary.]

Sorokina V.S. 2013b. Muscidae from above the tree-line in the Altai Mountains. In: Babin V.G., Tabakaev U.V., Dolgovykh S.V., Yurkova N.A., Aleynikova V.N. & Bondarenko A.V., Biodiversity, ecological issues of Gorny Altai and its neighboring regions: present, past and future: 118–122. Russia, Gorno-Altaisk. [In Russian, with English summary.]

Sorokina V.S. 2017. The Muscoidea Flies (Diptera) of the northern territories of Russia. Euroasian Entomological Journal 16(1): 44–56. [In Russian, with English summary.]

Sorokina V.S. 2018. Eleven new species of Spilogona Schnabl, 1911 (Diptera, Muscidae) from the Altai Mountains of Russia, with key to species. Zootaxa 4410 (2): 201–250. https://doi.org/10.11646/zootaxa.4410.2.1

Sorokina V.S. & Khruleva O.A. 2012. Details of species composition and distribution of house-flies (Diptera, Muscidae) of the Wrangel Island, Russia. Euroasian Entomological Journal 11: 553–564. [In Russian with English abstract.]

Tamura K., Stecher G., Peterson D., Filipski A. & Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197

Werner D. & Pont A.C. 2006a. The feeding and reproductive behavior of the Limnophorini (Diptera: Muscidae). Studia Dipterologica, Supplement, Halle/Saale 14: 79–114.

Werner D. & Pont A.C. 2006b. New results on Diptera predators in the blackfly plague areas of Central Europe and the Caucasus. Acta Entomologica Serbica, Supplement: 131–140.

Published
2018-12-11
How to Cite
Sorokina, V. S., & Shaikevich, E. V. (2018). The identification of the species of the ‘Spilogona contractifrons species-group’ and the ‘Spilogona nitidicauda species-group’ (Diptera, Muscidae) based on morphological and molecular analysis. European Journal of Taxonomy, (484). https://doi.org/10.5852/ejt.2018.484
Issue
No. 484 (2018): The identification of the species of the ‘Spilogona contractifrons species-group’ and the ‘Spilogona nitidicauda species-group’ (Diptera, Muscidae) based on morphological and molecular analysis
Section
Entomology
Copyright (c) 2018 Vera S. Sorokina, Elena V. Shaikevich

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