A new Myxobolus (Cnidaria: Myxosporea) infecting the ornamental catfish Corydoras schwartzi from the Purus River in Brazil

  • Patrick D. Mathews Department of Biophysics, Paulista Medical School, Federal University of São Paulo, 04023-062 São Paulo
  • Rafael R.M. Madrid Department of Biophysics, Paulista Medical School, Federal University of São Paulo, 04023-062 São Paulo
  • Omar Mertins Department of Biophysics, Paulista Medical School, Federal University of São Paulo, 04023-062 São Paulo
  • Vera L.S. Rigoni Department of Biophysics, Paulista Medical School, Federal University of São Paulo, 04023-062 São Paulo
  • André C. Morandini Department of Zoology, Institute of Biosciences, University of São Paulo, 05508-090 São Paulo
DOI: https://doi.org/10.5852/ejt.2020.620
Keywords: Amazon, intestinal infection, myxosporean, ultrastructure

Abstract

A new microscopic cnidarian is described, infecting five of thirty (16.6%) specimens of Corydoras schwartzi caught in the Purus River, State of Amazonas, Brazil. Histological analysis showed that cyst development occurred in the serosa layer of the intestine. Mature myxospores are ovoid in body shape in frontal view, 22.4 ± 0.3 μm in total length and 16.3 ± 0.1 μm in width. Internally, two aubergine-shaped, elongate symmetrical polar capsules occupy more than half the length of the spore, 14.3 ± 0.2 μm in length and 6.5 ± 0.1 μm in width. Ultrastructural analysis provided evidence of five polar filament coils inside the polar capsule and binucleated sporoplasm containing a moderate number of sporoplasmosomes. The valvogenic cells abutting each other form a sutural ridge and frequently a thin layer of homogeneous material separates the cells. The outer surface of the myxospore valves is smooth, with no evidence of formation of ridges in the valves. Immature myxospores in various stages of development were observed. This study is the first report of a myxosporean parasitizing C. schwartzi and the first report of a myxosporean infection in the intestine of an ornamental fish from South America.

References

Abrunhosa J., Sindeaux-Neto J.L., Dos Santos Â.K., Hamoy I. & Matos E. 2017. Myxobolus marajoensis sp. n. (Myxosporea: Myxobolidae), parasite of the freshwater catfish Rhamdia quelen from the Brazilian Amazon region. Revista Brasileira de Parasitologia Veterinária 26: 465–471. https://doi.org/10.1590/s1984-29612017067

Adriano E.A., Arana S., Ceccarelli P.S. & Cordeiro N.S. 2002. Light and scanning electron microscopy of Myxobolus porofilus sp. n. (Myxosporea: Myxobolidae) infecting the visceral cavity of Prochilodus lineatus (Pisces: Characiformes: Prochilodontidae) cultivated in Brazil. Folia Parasitologica 49 (4): 259–262. https://doi.org/10.14411/fp.2002.049

Adriano E.A., Arana S., Alves A.L., Silva M.R.M., Ceccarelli P.S., Henrique-Silva F. & Maia A.A.M. 2009. Myxobolus cordeiroi n. sp., a parasite of Zungaro jahu (Siluriformes: Pimelodidae) from Brazilian Pantanal: Morphology, phylogeny and histopathology. Veterinary Parasitology 162: 221–229. https://doi.org/10.1016/j.vetpar.2009.03.030

Atkinson S.D., Bartholomew J.L. & Lotan T. 2018. Myxozoans: Ancient metazoan parasites find a home in phylum Cnidaria. Zoology 129: 66–68. https://doi.org/10.1016/j.zool.2018.06.005

Barassa B., Arana S. & Cordeiro N.C. 2003. A new species of Henneguya, a gill parasite of Astyanax altiparanae (Pisces: Characidae) from Brazil, with comments on histopathology and seasonality. Memórias do Instituto Oswaldo Cruz 98: 761–765. https://doi.org/10.1590/S0074-02762003000600009

Camus A.C. & Griffin M.J. 2010. Molecular characterization and histopathology of Myxobolus koi infecting the gills of a koi, Cyprinus carpio, with an amended morphological description of the agent. Journal of Parasitology 96: 116–124. https://doi.org/10.1645/GE-2113.1

Camus A.C., Dill J.A., Rosser T.G., Pote L.M. & Griffin M.J. 2017. Myxobolus axelrodi n. sp. (Myxosporea: Myxobolidae) a parasite infecting the brain and retinas of the cardinal tetra Paracheirodon axelrodi (Teleostei: Characidae). Parasitology Research 116: 387–397. https://doi.org/10.1007/s00436-016-5301-1

Capodifoglio K.R.H., Adriano E.A., Silva M.R.M. & Maia A.A.M. 2019. The resolution of the taxonomic dilemma of Myxobolus colossomatis and description of two novel myxosporeans species of Colossoma macropomum from Amazon basin. Acta Tropica 191: 17–23. https://doi.org/10.1016/j.actatropica.2018.12.026

Crawshaw M.T. & Sweeting R.A. 1986. Myxobolus koi Kudo, 1919: a new record for Britain. Journal of Fish Diseases 9 (5): 465–467. https://doi.org/10.1111/j.1365-2761.1986.tb01041.x

Eiras J.C., Zhang J.Y. & Molnár K. 2014. Synopsis of the species of Myxobolus Butschli, 1882 (Myxozoa: Myxosporea, Myxobolidae) described between 2005 and 2013. Systematic Parasitology 88: 11–36. https://doi.org/10.1007/s11230-014-9484-5

Froese R. & Pauly D. 2011. Corydoras schwartzi. FishBase: World Wide Web electronic publication. Available from www.fishbase.org [accessed 10 Jan. 2019].

Hallett S.L., Hartigan A. & Atkinson S.D. 2015. Myxozoans on the move: dispersal modes, exotic species and emerging diseases. In: Okamura B., Gruhl A. & Bartholomew J.L. (eds) Myxozoan Evolution, Ecology and Development: 343–362. Springer, Cham, Switzerland.

Junk W.J., Soares M.G.M. & Bayley P.B. 2007. Freshwater fishes of the Amazon River basin: their biodiversity, fisheries, and habitats. Aquatic Ecosystem Health & Management 10: 153–173. https://doi.org/10.1080/14634980701351023

Lom J. & Arthur J.R. 1989. A guideline for the preparation of species descriptions in Myxosporea. Journal of Fish Diseases 12 (2): 151−156. https://doi.org/10.1111/j.1365-2761.1989.tb00287.x

Mathews P.D., Silva M.R.M, Maia A.A.M. & Adriano E.A. 2015. Ultrastructure and ssrRNA sequencing of Myxidium amazonense n. sp. a myxosporean parasite of Corydoras melini from the Rio Negro River, Amazonas state, Brazil. Parasitology Research 114: 4675–4683. https://doi.org/10.1007/s00436-015-4715-5

Mathews P.D., Maia A.A.M. & Adriano E.A. 2016. Morphological and ultrastructural aspects of Myxobolus niger n. sp. (Myxozoa) gill parasite of Corydoras melini (Siluriformes: Callichthyidae) from Brazilian Amazon. Acta Tropica 158: 214–219. https://doi.org/10.1016/j.actatropica.2016.03.016

Mathews P.D., Naldoni J. & Adriano E.A. 2017. Morphology and small subunit rDNA-based phylogeny of a new Henneguya species, infecting the ornamental fish Corydoras leucomelas from the Peruvian Amazon. Acta Tropica 176: 51–57. https://doi.org/10.1016/j.actatropica.2017.07.017

Mathews P.D., Mertins O., Pereira J.O.L., Maia A.A.M. & Adriano E.A. 2018. Morphology and 18S rDNA sequencing of Henneguya peruviensis n. sp. (Cnidaria: Myxosporea), a parasite of the Amazonian ornamental fish Hyphessobrycon loretoensis from Peru: A myxosporean dispersal approach. Acta Tropica 187: 207–213. https://doi.org/10.1016/j.actatropica.2018.08.012

Matos E., Videira M., Velasco M., Sanches O., Carmona C.S. & Matos P. 2014. Infection of the heart of Pimelodus ornatus (Teleostei, Pimelodidae), by Myxobolus sp. (Myxozoa, Myxobolidae). Revista Brasileira de Parasitologia Veterinária 23 (4): 543–546. https://doi.org/10.1590/s1984-29612014083

Molnár K. 2002. Site preference of fish myxosporeans in the gill. Diseases of Aquatic Organisms 48: 197–207. https://doi.org/10.3354/dao048197

Molnár K. & Békési L. 1993. Description of a new Myxobolus species: M. colossomatis n. sp. from the teleost Colossoma macropomum of the Amazon River basin. Journal of Applied Ichthyology 9: 57–63. https://doi.org/10.1111/j.1439-0426.1993.tb00388.x

Molnár K. & Eszterbauer E. 2015. Specificity of infection sites in vertebrate hosts. In: Okamura B., Gruhl A. & Bartholomew J.L. (eds) Myxozoan Evolution, Ecology and Development: 295–313. Springer, Cham, Switzerland.

Molnár K. & Székely C. 2014. Tissue preference of some myxobolids (Myxozoa: Myxosporea) from the musculature of European freshwater fishes. Diseases of Aquatic Organisms 107: 191–198. https://doi.org/10.3354/dao02688

Morand S. & Guégan J.F. 2000. Patterns of endemism in host-parasite associations: lessons from epidemiological models and comparative tests. Belgian Journal of Entomology 2: 135–147.

Moreau M.A. & Coomes O.T. 2007. Aquarium fish exploitation in western Amazonia: conservation issues in Peru. Environmental Conservation 34: 12–22. https://doi.org/10.1017/S0376892907003566

Naldoni J., Arana S., Maia A.A.M., Ceccarelli P.S., Tavares L.E.R., Borges F.A., Pozo C.F. & Adriano E.A. 2009. Henneguya pseudoplatystoma n. sp. causing reduction in epithelial area of gills in the farmed pintado, a South American catfish: histopathology and ultrastructure. Veterinary Parasitology 166: 52–59. https://doi.org/10.1016/j.vetpar.2009.07.034

Naldoni J., Maia A.A.M., Correa L.L., Da Silva M.R.M. & Adriano E.A. 2018. New myxosporeans parasitizing Phractocephalus hemioliopterus from Brazil: morphology, ultrastructure and SSU-rDNA sequencing. Diseases of Aquatic Organisms 128: 37–49. https://doi.org/10.3354/dao03210

Okamura B., Gruhl A. & Bartholomew J.L. 2015. An introduction to Myxozoan evolution, ecology and development. In: Okamura B., Gruhl A. & Bartholomew J.L. (eds) Myxozoan Evolution, Ecology and Development:1–20. Springer, Cham, Switzerland.

Padrós F., Palenzuela O., Hispano C., Tosas O., Zarza C., Crespo S. & Alvarez-Pellitero P. 2001. Myxidium leei (Myxozoa) infections in aquarium-reared Mediterranean fish species. Diseases of Aquatic Organisms 47: 57–62. https://doi.org/10.3354/dao047057

Penido J.C.N. 1927. Quelques nouvelles myxosporidies parasites des poissons d’eau douce du Brésil. Comptes Rendus de la Société brésilienne de Sciences naturelles 97: 850–852.

Prang G. 2007. An industry analysis of the freshwater ornamental fishery with particular reference to the supply of Brazilian freshwater ornamentals to the UK market. Uakari 3: 7–51. https://doi.org/10.31420/uakari.v3i1.18

Reis R.E. 1998. Systematics, biogeography, and the fossil record of the Callichthyidae: a review of the available data. In: Malabarba L.R., Reis R.E., Vari R.P., Lucena Z.M.S. & Lucena C.A.S. (eds) Phylogeny and Classification of Neotropical Fishes: 351–362. Edipucrs, Porto Alegre, Brazil.

Saha M. & Bandyopadhyay P.K. 2017. Studies on histopathological alteration of three major organs of the goldfish, Carassius auratus L., of India due to myxozoan infection with special reference to scanning electron microscopic observation. Parasitology Research 116: 511–520. https://doi.org/10.1007/s00436-016-5314-9

Sindeaux-Neto J.L., Velasco M., Da Silva J.M.V, Dos Santos P.F.S., Sanches O., Matos P. & Matos E. 2016. Lymphocytic meningoencephalomyelitis associated with Myxobolus sp. (Bivalvulidae: Myxozoa) infection in the Amazonian fish Eigenmannia sp. (Sternopygidae: Gymnotiformes). Revista Brasileira de Parasitologia Veteterinária 25: 158–162. https://doi.org/10.1590/S1984-29612016023

Velasco M., Videira M., Nascimento L.C., Matos P., Gonçalves E.C. & Matos E. 2016. Henneguya paraensis n. sp. (Myxozoa; Myxosporea), a new gill parasite of the Amazonian fish Cichla temensis (Teleostei: Cichlidae): morphological and molecular aspects. Parasitology Research 115: 1779–1787. https://doi.org/10.1007/s00436-016-4916-6

Vidal L.P., Iannacone J., Whipps C.M. & Luque J.L. 2017. Synopsis of the species of Myxozoa Grassé, 1970 (Cnidaria: Myxosporea) in the Americas. Neotropical Helminthology 11: 413–511.

Wang M., Zhao Y. & Yang C. 2019. The impacts of geographic and host species isolation on population divergence of Myxobolus lentisuturalis. Parasitology Research 118: 1061–1066. https://doi.org/10.1007/s00436-019-06234-9

Whipps C.M. & Kent M.L. 2006. Phylogeography of the cosmopolitan marine parasite Kudoa thyrsites (Myxozoa: Myxosporea). Journal of Eukaryotic Microbiology 53: 364–373. https://doi.org/10.1111/j.1550-7408.2006.00114.x

Zatti S.A., Atkinson S.D., Maia A.A.M., Bartholomew J.L. & Adriano E.A. 2018a. Novel Henneguya spp. (Cnidaria: Myxozoa) from cichlid fish in the Amazon basin cluster by geographic origin. Parasitology Research 117: 849–859. https://doi.org/10.1007/s00436-018-5762-5

Zatti S.A., Atkinson S.D., Maia A.A.M., Bartholomew J.L. & Adriano E.A. 2018b. Ceratomyxa gracillima n. sp. (Cnidaria: Myxosporea) provides evidence of panmixia and ceratomyxid radiation in the Amazon basin. Parasitology 145: 1137–1146. https://doi.org/10.1017/S0031182017002323

Zhang Z.Q. 2013. Animal biodiversity: an update of classification and diversity in 2013. Zootaxa 3703: 5–11. https://doi.org/10.11646/zootaxa.3703.1.3

Published
2020-03-30
How to Cite
Mathews, P. D., Madrid, R. R., Mertins, O., Rigoni, V. L., & Morandini, A. C. (2020). A new Myxobolus (Cnidaria: Myxosporea) infecting the ornamental catfish Corydoras schwartzi from the Purus River in Brazil. European Journal of Taxonomy, (620). https://doi.org/10.5852/ejt.2020.620
Issue
No. 620 (2020): A new Myxobolus (Cnidaria: Myxosporea) infecting the ornamental catfish Corydoras schwartzi from the Purus River in Brazil
Section
Zoology

Copyright (c) 2020 Patrick D. Mathews, Rafael R.M. Madrid, Omar Mertins, Vera L.S. Rigoni, André C. Morandini

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