A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae)

  • José B. Azofeifa-Bolaños Universidad Nacional de Costa Rica, Instituto de Investigación y Servicios Forestales, Heredia
  • L. Rodolphe Gigant Université de La Réunion, UMR PVBMT, Saint Denis, La Réunion
  • Mayra Nicolás-García Instituto Tecnológico de Tuxtepec, Tuxtepec (Oax)
  • Marc Pignal Muséum national d’Histoire naturelle, UMR 7205 – ISYEB CNRS-MNHN-UPMC-EPHE, Paris
  • Fabiola B. Tavares-González Instituto Tecnológico de Veracruz, Veracruz (Ver)
  • Eric Hágsater Herbario AMO, México (DF)
  • Gerardo A. Salazar-Chávez Universidad Autónoma de México, Instituto de Biología, México (DF)
  • Delfino Reyes-López Benemérita Universidad Autónoma de Puebla, Facultad de Ingeniería Agro-hidráulica, Teziutlán (Pue)
  • Fredy L. Archila-Morales Herbario BIGU, Universidad de San Carlos de Guatemala
  • José A. García-García Universidad Nacional de Costa Rica, Escuela de Ciencias Agrarias, Heredia
  • Denis da Silva Université de La Réunion, UMR PVBMT, Saint Denis, La Réunion
  • Agathe Allibert CIRAD, UMR PVBMT, Saint Pierre, La Réunion
  • Frank Solano-Campos Universidad Nacional de Costa Rica, Laboratorio de Biotecnología de Plantas, Heredia
  • Guadalupe del Carmen Rodríguez-Jimenes Instituto Tecnológico de Veracruz, Veracruz (Ver)
  • Amelia Paniagua-Vásquez Universidad Nacional de Costa Rica, Instituto de Investigación y Servicios Forestales, Heredia
  • Pascale Besse Université de La Réunion, UMR PVBMT, Saint Denis, La Réunion
  • Araceli Pérez-Silva Instituto Tecnológico de Tuxtepec, Tuxtepec (Oax)
  • Michel Grisoni CIRAD, UMR PVBMT, Saint Pierre, La Réunion
DOI: https://doi.org/10.5852/ejt.2017.284
Keywords: barcoding, Costa Rica, Limón, radiation, Vanilla planifolia, V. sotoarenasii

Abstract

We describe a new vanilla species growing in sympatry with Vanilla planifolia Jacks. ex Andrews (Orchidaceae) in the province of Limón, Caribbean coast of Costa Rica. The morphology of the reproductive and vegetative organs observed on vines cultivated under shade-house, the nuclear (Internal Transcribed Spacer) and plastid (matK) nucleotide sequences, as well as the contents of aromatic compounds measured in ripe fruits, show that this species is close to but distinct from V. planifolia. The name V. sotoarenasii M.Pignal, Azofeifa-Bolaños & Grisoni sp. nov. is proposed for this new Vanilla species endemic in Costa Rica. It is especially distinguished from V. planifolia by a reduction of about 30% of the size of the fruits and flowers, by a divergence of ITS sequences for at least two species-conserved nucleotides compared to seven other species of the V. planifolia group, and by the presence of anisic compounds and low content of phenolic compounds (including vanillin) in the fruits. These results confirmed the extension of the area of distribution of V. planifolia southward to Costa Rica, where a recent speciation process occurred. Because of its particular agronomic and aromatic properties, V. sotoarenasii sp. nov. could represent a valuable biological resource for the vanilla industry.

References

Ackerman J.D. 1986. Mechanisms and evolution of food-deceptive pollination systems in orchids. Lindleyana 1: 108–113.

Agardh C.A., Holmberg L.P. & Lundstrom P.M. 1825. Classes Plantarum. Literis Berlingianis, Lundae [Lund, Sweden].

Ames O. 1934. An addition to the genus Vanilla. Harvard University Botanical Museum Leaflet 2: 101–102.

Andrews H.C. 1808. Vanilla planifolia. Botanist’s Repository 8: 538.

Archila F. & Chiron G.R. 2012. Addition à la flore du Guatemala: Vanilla esquipulensis (Orchidaceae), espèce des forêts xérophytes. Richardiana 13: 3–12.

Azofeifa-Bolaños J.B., Paniagua-Vásquez A. & Garcia-Garcia J.A. 2014. Importancia y desafíos de la conservación de Vanilla spp. (Orchidaceae) en Costa Rica. Agronomía Mesoamericana 25: 189–202. http://dx.doi.org/10.15517/am.v25i1.14220

Belanger F.C. & Havkin-Frenkel D. 2011. Molecular analysis of a Vanilla hybrid cultivated in Costa Rica. In: Havkin-Frenkel D. & Belanger F.C. (eds) Handbook of Vanilla Science and Technology: 256–263. Wiley-Blackwell, Chichester, United Kingdom.

Besse P. (ed.) 2014. Molecular Plant Taxonomy – Methods and Protocols. Humana Press (Springer), New York.

Blume C.L.V. 1835. Rumphia, sive, Commentationes botanicæ imprimis de plantis Indiæ Orientalis: tum penitus incognitis tum quæ in libris Rheedii, Rumphi, Roxburghii, Wallichii aliorum recensentur / scripsit C.L. Blume cognomine Rumphius. Vol. 1: 196. Leiden. Available from http://biodiversitylibrary.org/page/41039881 [accessed 18 Jan. 2017].

Bory S., Lubinsky P., Risterucci A.-M., Noyer J.-L., Grisoni M., Duval M.-F. & Besse P. 2008. Patterns of introduction and diversification of Vanilla planifolia (Orchidaceae) in Reunion Island (Indian Ocean). American Journal of Botany 95: 805–815. http://dx.doi.org/10.3732/ajb.2007332

Bouétard A., Lefeuvre P., Gigant R., Bory S., Pignal M., Besse P. & Grisoni M. 2010. Evidence of transoceanic dispersion of the genus Vanilla based on plastid DNA phylogenetic analysis. Molecular Phylogenetics and Evolution 55: 621–630. http://dx.doi.org/10.1016/j.ympev.2010.01.021

Brunschwig C., Collard F.X., Bianchini J.P. & Raharivelomanana P. 2009. Evaluation of chemical variability of cured vanilla beans (Vanilla tahitensis and Vanilla planifolia). Natural Products Communications 4: 1393–1400.

Cameron K.M. 2009. On the value of nuclear and mitochondrial gene sequences for reconstructing the phylogeny of vanilloid orchids (Vanilloideae, Orchidaceae). Annals of Botany 104: 377–385. http://dx.doi.org/10.1093/aob/mcp024

Cameron K.M. 2010. Vanilloid orchids. In: Odoux E. & Grisoni M. (eds) Vanilla: 1–13. CRC Press, Boca Raton, FL.

Cameron K.M. 2011. Vanilla phylogeny and classification. In: Havkin-Frenkel D. & Belanger F.C. (eds) Handbook of Vanilla Science and Technology: 241–255. Wiley-Blackwell, Chichester, United Kingdom.

Costantin D. & Bois J. 1915. Sur trois types de vanilles commerciales de Tahiti. Comptes Rendus de l’Académie des Sciences de Paris 161: 196–202.

Darriba D., Taboada G., Doallo R. & Posada D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. http://dx.doi.org/10.1038/nmeth.2109

Divakaran M., Babu K.N. & Peter K.V. 2006. Conservation of Vanilla species in vitro. Scientia Horticulturae 110: 175–180. http://dx.doi.org/10.1016/j.scienta.2006.07.003

Gigant R., Bory S., Grisoni M. & Besse P. 2011. Biodiversity and evolution in the Vanilla genus. In: Grillo O. & Venora G. (eds) The Dynamical Processes of Biodiversity: Case Studies of Evolution and Spatial Distribution. InTech, Rijeka, Croatia.

Guindon S., Gascuel O. & Rannala B. 2003. A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology 52: 696–704. http://dx.doi.org/10.1080/10635150390235520

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Heller A.H. & Hawkes A.D. 1966. Nicaraguan orchid studies. Phytologia 14: 19–20.

Hoehne F.C. 1910. Botânica: I. Bromeliaceas, Pontederiaceas, Liliaceas, Amaryllidaceas, Iridaceas, Orchidaceas, Aristolochiaceas, Droseraceas e Passifloraceas. Commissão das Linhas Telegraficas Estratégicas, Matto Grosso e Amazonas 5: 1–71.

Hoehne F.C. 1944. Vanilla trigonocarpa. Arquivos de Botânica do Estado de São Paulo 1 (6): 126–127.

Hoehne F.C. 1950. Algunas novidades da flora do Brasil austro-oriental de entre Orchidaceas e Convolvulaceas. Arquivos de Botânica do Estado de São Paulo 2 (5): 105–110.

Hollingsworth P.M., Forrest L.L., Spouge J.L., Hajibabaei M., Ratnasingham S., van der Bank M., Chase M.W., Cowan R.S., Erickson D.L., Fazekas A.J., Graham S.W., James K.E., Kim K.-J., Kress W.J., Schneider H., Van AlphenStahl J., Barrett S.C.H., van den Berg C., Bogarin D., Burgess K.S., Cameron K.M., Carine M., Chacón J., Clark A., Clarkson J.J., Conrad F., Devey D.S., Ford C.S., Hedderson T.A.J., Hollingsworth M.L., Husband B.C., Kelly L.J., Kesanakurti P.R., Kim J.S., Kim Y.-D., Lahaye R., Lee H.-L., Long D.G., Madriñán S., Maurin O., Meusnier I., Newmaster S.G., Park C.-W., Percy D.M., Petersen G., Richardson J.E., Salazar G.A., Savolainen V., Seberg O., Wilkinson M.J., Yi D.-K. & Little D.P. 2009. A DNA barcode for land plants. Proceedings of the National Academy of Sciences 106: 12794–12797. http://dx.doi.org/10.1073/pnas.0905845106

Hothorn T., Bretz F. & Westfall P. 2008. Simultaneous inference in general parametric models. Biometrical Journal 50: 346–336. http://dx.doi.org/10.1002/bimj.200810425

IUCN 2012. IUCN Red List Categories and Criteria: Version 3.1. Second edition. IUCN, Gland, Switzerland and Cambridge, United Kingdom.

Jersáková J., Johnson S. & Kindlmann P. 2006. Mechanisms and evolution of deceptive pollination in orchids. Biological Reviews of the Cambridge Philosophical Society 81: 219–235. http://dx.doi.org/10.1017/S1464793105006986

Jussieu A.-L. de 1789. Genera Plantarum, Secundum Ordines Naturales Disposita, juxta Methodum in Horto Regio Parisiensi Exaratam. Herissant, Paris. Available from http://biodiversitylibrary.org/page/5437140 [accessed 12 Jan. 2017].

Knudson L. 1950. Germination of seeds of vanilla. American Journal of Botany 37: 241–247.

Koyyappurath S., Conéjéro G., Dijoux J.B., Lapeyre Montes F., Jade K., Chiroleu F., Gatineau F., Verdeil J.L., Besse P. & Grisoni M. 2015. Differential responses of vanilla accessions to root rot and colonization by Fusarium oxysporum f. sp. radicis-vanillae. Frontiers in Plant Science 6. http://dx.doi.org/10.3389/fpls.2015.01125

Kumar S., Stecher G. & Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 (7): 1870–1874. http://dx.doi.org/10.1093/molbev/msw054

Link H.F. 1829. Handbuch zur Erkennung der nutzbarsten und am häufigsten vorkommenden Gewächse. Haude und Spener, Berlin.

Lubinsky P., Van Dam M. & Van Dam A. 2006. Pollination of Vanilla and evolution in Orchidaceae. Lindleyana 75: 926–929.

Lubinsky P., Cameron K.M., Molina M.C., Wong M., Lepers-Andrzejewski S., Gómez-Pompa A. & Kim S.-C. 2008. Neotropical roots of a Polynesian spice: the hybrid origin of Tahitian vanilla, Vanilla tahitensis (Orchidaceae). American Journal of Botany 95: 1040–1047. http://dx.doi.org/10.3732/ajb.0800067

Maruenda H., Vico M.D.L., Householder J.E., Janovec J.P., Cañari C., Naka A. & Gonzalez A.E. 2013. Exploration of Vanilla pompona from the Peruvian Amazon as a potential source of vanilla essence: quantification of phenolics by HPLC-DAD. Food Chemistry 138: 161–167. http://dx.doi.org/10.1016/j.foodchem.2012.10.037

Miller P. 1754. The Gardeners Dictionary: Containing the Methods of Cultivating and Improving all Sorts of Trees, Plants, and Flowers for the Kitchen, Fruit and Pleasure Gardens and also those which are used in Medecine. Fourth edition. J. & J. Rivington, London.

Molineros-Hurtado F., González-Mina R.T., Flanagan N.S. & Otero J.T. 2014. Vanilla rivasii (Orchidaceae), a new species from the Colombian Pacific region. Lankesteriana 13: 353–357.

Moore J.W. 1933. New and critical plants from Raiatea. Bulletin of the Bernice P. Bishop Museum 102: 1–53.

Nielsen L.R. 2000. Natural hybridization between Vanilla claviculata (W.Wright) Sw. and V. barbellata Rchb.f. (Orchidaceae): genetic, morphological, and pollination experimental data. Botanical Journal of the Linnean Society 133: 285–302. http://dx.doi.org/10.1111/j.1095-8339.2000.tb01547.x

Pabst G.F.J. 1975. Vanilla dungsii. Bradea 2: 49.

Palama T.L., Khatib A., Choi Y.H., Payet B., Fock I., Verpoorte R. & Kodja H. 2009. Metabolic changes in different developmental stages of Vanilla planifolia pods. Journal of Agricultural and Food Chemistry 57: 7651–7658. http://dx.doi.org/10.1021/jf901508f

Pansarin E.R. & Pansarin L.M. 2014. Floral biology of two Vanilloideae (Orchidaceae) primarily adapted to pollination by euglossine bees. Plant Biology 16: 1104–1113. http://dx.doi.org/10.1111/plb.12160

Pansarin E.R., Salatino A., Pansarin L.M. & Sazima M. 2012. Pollination systems in Pogonieae (Orchidaceae: Vanilloideae): A hypothesis of evolution among reward and rewardless flowers. Flora – Morphology, Distribution, Functional Ecology of Plants 207: 849–861. http://dx.doi.org/10.1016/j.flora.2012.09.011

Pérez-Silva A., Odoux E., Brat P., Ribeyre F., Rodriguez-Jimenes G., Robles-Olvera V., García-Alvarado M.A. & Günata Z. 2006. GC–MS and GC–olfactometry analysis of aroma compounds in a representative organic aroma extract from cured vanilla (Vanilla planifolia G. Jackson) beans. Food Chemistry 99: 728–735. http://dx.doi.org/10.1016/j.foodchem.2005.08.050

Pérez Silva A., Gunata Z., Lepoutre J.-P. & Odoux E. 2011. New insight on the genesis and fate of odor-active compounds in vanilla beans (Vanilla planifolia G. Jackson) during traditional curing. Food Research International 44: 2930–2937. http://dx.doi.org/10.1016/j.foodres.2011.06.048

Portères R. 1953. Le potentiel de variation clonale de Vanilla tahitensis J. W. Moore. L’agronomie Tropicale 8: 639–640.

Portères R. 1954. Le genre Vanilla et ses espèces. In: G. Bouriquet (ed.) Le Vanillier et la Vanille dans le Monde: 94–290. Editions Paul Lechevalier, Paris.

Presl C.B. von 1827. Reliquiae Haenkeanae. J.G. Calve, Prague, Czech Republic.

R Core Team 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna.

Ramírez S.R., Gravendeel B., Singer R.B., Marshall C.R. & Pierce N.E. 2007. Dating the origin of the Orchidaceae from a fossil orchid with its pollinator. Nature 448: 1042–1045. http://dx.doi.org/10.1038/nature06039

Reichenbach F.H.G. 1865. Vanilla phaeantha. Flora 48: 274.

Reyes-López D., Flores-Jiménez A., Huerta-Lara M., Kelso-Bucio H.A., Avendaño-Arrazate C.H., Lobato-Ortiz R., Aragón-García A. & López-Olguín J.F. 2014. Variación morfométrica de fruto y semilla en cuatro especies del género Vanilla. Ecosistemas y Recursos Agropecuarios 1: 205–218.

Rolfe R.A. 1895. Vanillas of commerce. Bulletin of Miscellaneous Information, Royal Botanic Gardens, Kew 1895: 169–178. Available from http://biodiversitylibrary.org/page/40984721 [accessed 12 Jan. 2017].

Rolfe R.A. 1899. New Orchids. – Decades 23 and 24. Bulletin of Miscellaneous Information, Royal Botanic Gardens, Kew 1899: 126–133. Available from http://biodiversitylibrary.org/page/40848484 [accessed 12 Jan. 2017].

Roux-Cuvelier M. & Grisoni M. 2010. Conservation and movement of Vanilla germplasm. In: Odoux E. & Grisoni M. (eds) Vanilla: 31–41. CRC Press, Boca Raton, FL.

Sambin A. & Chiron G.R. 2015. Deux nouvelles espèces de Vanilla (Orchidaceae) de Guyane française. Richardiana 15: 306–316.

Schiede C.J.W. 1829. Botanische Berichte aus Mexico. Linnaea 4: 574–604.

Soto Arenas M.A. & Cribb P. 2010. A new infrageneric classification and synopsis of the genus Vanilla Plum. ex Mill. (Orchidaceae: Vanillinae). Lankesteriana 9: 355–398.

Soto Arenas M.A. & Dressler R.L. 2010. A revision of the Mexican and Central American species of Vanilla Plumier ex Miller with a characterization of their ITS region of the nuclear ribosomal DNA. Lankesteriana 9: 285–354.

Sun H.-Q., Cheng J., Zhang F.-M., Luo Y.-B. & Ge S. 2009. Reproductive success of non-rewarding Cypripedium japonicum benefits from low spatial dispersion pattern and asynchronous flowering. Annals of Botany 103: 1227–1237. http://dx.doi.org/10.1093/aob/mcp066

Sun Y., Skinner D.Z., Liang G.H. & Hulbert S.H. 1994. Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA. Theoretical and Applied Genetics 89: 26–32. http://dx.doi.org/10.1007/BF00226978

Szlachetko D.L. 1995. Systema Orchidalium. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.

Takhtajan A.L. 1967. Sistema i Filogeniia Tsvetkovykh Rastenii (Systema et Phylogenia Magnoliophytorum). Soviet Science Press, Moscow.

Theis T. & Jiménez T.A. 1957. A Vanilla hybrid resistant to Fusarium root rot. Phytopathology 47: 578–581.

Thiers B. Continuously updated. Index Herbariorum, a Global Directory of Public Herbaria and Associated Staff. Available from http://sweetgum.nybg.org/ih/ [accessed Aug. 2016].

Varela Quirós E. 2011. Vanilla production in Costa Rica. In: Havkin-Frenkel D. & Belanger F.C. (eds) Handbook of Vanilla Science and Technology: 40–49. Wiley-Blackwell, Chichester, United Kingdom.

Xu S., Li D., Li J., Xiang X., Jin W., Huang W., Jin X. & Huang L. 2015. Evaluation of the DNA barcodes in Dendrobium (Orchidaceae) from mainland Asia. PLoS ONE 10: e0115168. http://dx.doi.org/10.1371/journal.pone.0115168

Published
2017-02-22
How to Cite
Azofeifa-Bolaños, J. B., Gigant, L. R., Nicolás-García, M., Pignal, M., Tavares-González, F. B., Hágsater, E., Salazar-Chávez, G. A., Reyes-López, D., Archila-Morales, F. L., García-García, J. A., da Silva, D., Allibert, A., Solano-Campos, F., Rodríguez-Jimenes, G. del C., Paniagua-Vásquez, A., Besse, P., Pérez-Silva, A., & Grisoni, M. (2017). A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae). European Journal of Taxonomy, (284). https://doi.org/10.5852/ejt.2017.284
Issue
No. 284 (2017): A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae)
Section
Botany
Copyright (c) 2017 José B. Azofeifa-Bolaños, L. Rodolphe Gigant, Mayra Nicolás-García, Marc Pignal, Fabiola B. Tavares-González, Eric Hágsater, Gerardo A. Salazar-Chávez, Delfino Reyes-López, Fredy L. Archila-Morales, José A. García-García, Denis da Silva, Agathe Allibert, Frank Solano-Campos, Guadalupe del Carmen Rodríguez-Jimenes, Amelia Paniagua-Vásquez, Pascale Besse, Araceli Pérez-Silva, Michel Grisoni

Creative Commons Copyright Notices

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are NOT ALLOWED TO post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to taxonomic issues.