Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2)

  • Claudia Isabel Navarro-Rodríguez Laboratory of Arachnology (LATLAX), Laboratorio Regional de Biodiversidad y Cultivo de Tejidos Vegetales (LBCTV), Instituto de Biología, Universidad Nacional Autónoma de Mexico (UNAM), sede Tlaxcala, Ex-Fábrica San Manuel, San Miguel Contla, 90640 Santa Cruz Tlaxcala, Tlaxcala
  • Alejandro Valdez-Mondragón CONACYT Research Fellow. Laboratory of Arachnology (LATLAX), Laboratorio Regional de Biodiversidad y Cultivo de Tejidos Vegetales (LBCTV), Instituto de Biología, Universidad Nacional Autónoma de Mexico (UNAM), sede Tlaxcala, Ex-Fábrica San Manuel, San Miguel Contla, 90640 Santa Cruz Tlaxcala, Tlaxcala
DOI: https://doi.org/10.5852/ejt.2020.704
Keywords: integrative taxonomy, molecular markers, Synspermiata, species delimitation, Hidalgo

Abstract

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.

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Published
2020-08-19
How to Cite
Navarro-Rodríguez, C. I., & Valdez-Mondragón, A. (2020). Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2). European Journal of Taxonomy, (704). https://doi.org/10.5852/ejt.2020.704
Issue
No. 704 (2020)
Section
Zoology

Copyright (c) 2020 Claudia Isabel Navarro-Rodríguez, Alejandro Valdez-Mondragón

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