A new species of Mesopolobus (Hymenoptera, Pteromalidae) from black locust crops

  • Zoltán László Hungarian Department of Biology and Ecology, Babeş-Bolyai University, Str. Clinicilor nr. 5–7, 400006 Cluj-Napoca, Romania https://orcid.org/0000-0001-5064-4785
  • K. Tímea Lakatos Department of Ecology, University of Debrecen, Debrecen, Egyetem square 1, H-4032, Hungary
  • Avar-Lehel Dénes Hungarian Department of Biology and Ecology, Babeş-Bolyai University, Str. Clinicilor nr. 5–7, 400006 Cluj-Napoca, Romania
Keywords: new species description, black locust seed predation, seed predator parasitoid, morphometry, multivariate ratio analysis


A new species of the genus Mesopolobus Westwood, 1833, Mesopolobus robiniae Lakatos & László sp. nov., is described and illustrated from east-central Europe (Romania and Hungary). The species was reared from black locust (Robinia pseudoacacia) seedpod samples, where it most likely parasitizes the black locust’s seed predator Bruchophagus robiniae Zerova, 1970. Here we present the new species and report on its ecological relationships within the European seed predator community of black locust. We also give details regarding type material and type locality, a detailed description with images, a differential diagnosis of the new species, and a modification to the identification key published by Graham (1969), that distinguishes this new species from closely related species. In addition, we provide information on the distribution, biology and results of barcoding analysis. We also provide the DNA sequence data to complement the morphological taxonomy.


Askew R.R. 1961. A study of the biology of species of the genus Mesopolobus (Hymenoptera: Pteromalidae) associated with cynipid galls on oak. Transactions of the Royal Entomological Society of London 113 (8): 155–173. https://doi.org/10.1111/j.1365-2311.1961.tb00806.x

Baur H. & Leuenberger C. 2011. Analysis of ratios in multivariate morphometry. Systematic Biology 60 (6): 813–825. https://doi.org/10.1093/sysbio/syr061

Baur H., Muller F.J., Gibson G.A.P., Mason P.G. & Kuhlmann U. 2007. A review of the species of Mesopolobus (Chalcidoidea: Pteromalidae) associated with Ceutorhynchus (Coleoptera: Curculionidae) host-species of European origin. Bulletin of Entomological Research 97 (4): 387–397. https://doi.org/10.1017/S0007485307005032

Baur H., Kranz-Baltensperger Y., Cruaud A., Rasplus J.Y., Timokhov A.V. & Gokhman V.E. 2014. Morphometric analysis and taxonomic revision of Anisopteromalus Ruschka (Hymenoptera: Chalcidoidea: Pteromalidae) – an integrative approach. Systematic Entomology 39 (4): 691–709. https://doi.org/10.1111/syen.12081

Benesperi R., Giuliani C., Zanetti S., Gennai M., Mariotti Lippi M., Guidi T., Nascimbene J. & Foggi B. 2012. Forest plant diversity is threatened by Robinia pseudoacacia (black-locust) invasion. Biodiversity and Conservation 21 (14): 3555–3568. https://doi.org/10.1007/s10531-012-0380-5

Bouček Z. & Rasplus J.-Y. 1991. Illustrated Key to West-Palearctic Genera of Pteromalidae (Hymenoptera: Chalcidoidea). Institut national de la Recherche agronomique (INRA), Paris.

Cronin J. & Abrahamson W.G. 2001. Do parasitoids diversify in response to host-plant shifts by herbivorous insects? Ecological Entomology 26 (4): 347–355. https://doi.org/10.1046/j.1365-2311.2001.00332.x

Daisie 2009. Handbook of Alien Species in Europe. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8280-1

Dickie I.A., Bennett B.M., Burrows L.E., Nuñez M.A., Peltzer D.A., Porté A., Richardson D.M., Rejmánek M., Rundel P.W. & van Wilgen B.W. 2014. Conflicting values: ecosystem services and invasive tree management. Biological Invasions 16 (3): 705–719. https://doi.org/10.1007/s10530-013-0609-6

Doganlar M. 1979. Two new species of Mesopolobus Westwood (Hymenoptera: Pteromalidae) from western Canada. The Canadian Entomologist 111 (6): 649–659. https://doi.org/10.4039/Ent111649-6

Enescu C.M. & Dănescu A. 2013. An invasive neophyte in the conventional land reclamation flora in Romania. Bulletin of the Transilvania University of Braşov Series II: Forestry, Wood Industry, Agricultural Food Engineering 6 (55) (2): 23–30.

Farkas K. & Terpó-Pomogyi M. 1974. A new species of the Hungarian fauna: Bruchophagus robiniae (Hymenoptera, Eurytomidae). Növényvédelem (Plant Protection) 10 (11): 507–508.

Folmer O., Black M., Hoeh W., Lutz R. & Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3 (5): 294–299.

Gahan A.B. 1932. Miscellaneous descriptions and notes on parasitic Hymenoptera. Annals of the Entomological Society of America 25 (4): 736–757. https://doi.org/10.1093/aesa/25.4.736

Graham M.W.R. de V. 1969. The Pteromalidae of North-Western Europe. Bulletin of the British Museum (Natural History) Entomology Suppl. 16: 1–909.

Hall T. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Hanzelka J. & Reif J. 2015. Responses to the black locust (Robinia pseudoacacia) invasion differ between habitat specialists and generalists in central European forest birds. Journal of Ornithology 156 (4): 1015–1024. https://doi.org/10.1007/s10336-015-1231-4

Johnson M., Zaretskaya I., Raytselis Y., Merezhuk Y., McGinnis S. & Madden T.L. 2008. NCBI BLAST: a better web interface. Nucleic Acids Research 36 (Web Server issue): 5–9. https://doi.org/10.1093/nar/gkn201

Kleinbauer I., Dullinger S., Peterseil J. & Essl F. 2010. Climate change might drive the invasive tree Robinia pseudacacia into nature reserves and endangered habitats. Biological Conservation 143 (2): 382–390. https://doi.org/10.1016/j.biocon.2009.10.024

Kumar S., Stecher G., Li M., Knyaz C. & Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35 (6): 1547–1549. https://doi.org/10.1093/molbev/msy096

Lakatos K.T., László Z. & Tóthmérész B. 2016. Resource dependence in a new ecosystem: a host plant and its colonizing community. Acta Oecologica 73: 80–86. https://doi.org/10.1016/j.actao.2016.03.003

Lakatos K.T., László Z. & Tóthmérész B. 2018. Disturbance induced dynamics of a tritrophic novel ecosystem. Bulletin of Entomological Research 108 (2): 158–165. https://doi.org/10.1017/S0007485317000621

Lazzaro L., Mazza G., D’Errico G., Fabiani A., Giuliani C., Inghilesi A.F., Lagomarsino A., Landi S., Lastrucci L., Pastorelli R., Roversi P.F., Torrini G., Tricarico E. & Foggi B. 2018. How ecosystems change following invasion by Robinia pseudoacacia: insights from soil chemical properties and soil microbial, nematode, microarthropod and plant communities. Science of the Total Environment 622–623: 1509–1518. https://doi.org/10.1016/j.scitotenv.2017.10.017

Narendran T.C., Khan F.R. & Akhtar M.S. 2011. On a new species of Mesopolobus Westwood (Hymenoptera: Pteromalidae ) from India with a key to the Indian species. Oriental Insects 45 (2–3): 127–131. https://doi.org/10.1080/00305316.2011.590641

Nieves-Aldrey J.L. 1983. Sobre las especies del género Mesopolobus (Hym. Pteromalidae) asociadas con agallas de cinípidos en Quercus spp en Salamanca. Boletín de la Asociación española de Entomología 7: 9–18. Available from http://www.entomologica.es/publicaciones-boletin/art206 [accessed 17 Feb. 2021].

Norton E. 1868. Catalogue of the described Tenthredinidæ and Uroceridæ of North America. (Continued). Transactions of the American Entomological Society (1867-1877) 2: 321–368. https://doi.org/10.2307/25076221

Noyes J. 2020. Universal Chalcidoidea Database. Available from http://www.nhm.ac.uk/chalcidoids [accessed 17 Feb. 2021].

Perju T. 1998. The pest of the white acacia (Robinia pseudoacacia L.). Buletin de Informare Societatea Lepidopterologica Romana 9 (3–4): 291–295.

Pujade-Villar J. 1993. Especies de Mesopolobus (Hym., Pteromalidae) asociadas a agallas de Cynipini (Hym., Cynipidae) del nordeste ibérico y notas sobre la validez de M. lichtensteini (Mayr, 1903). Eos: Revista española de Entomología 69 (1): 63–73. Available from http://hdl.handle.net/10261/174605 [accessed 17 Feb. 2021].

R Core Team 2020. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Available from https://www.R-project.org/ [accessed 18 Mar. 2021].

Redei K., Osváth-Bujtás Z. & Balla I. 2001. Propagation methods for black locust (Robinia pseudoacacia L.) improvement in Hungary. Journal of Forestry Research 12 (4): 215–219. https://doi.org/10.1007/bf02856710

Ronquist F., Teslenko M., Van Der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A. & Huelsenbeck J.P. 2012. MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029

Sádlo J., Vítková M., Pergl J. & Pyšek P. 2017. Towards site-specific management of invasive alien trees based on the assessment of their impacts: the case of Robinia pseudoacacia. NeoBiota 35: 1–34. https://doi.org/10.3897/neobiota.35.11909

Stone K.R. 2009. Robinia pseudoacacia. In: Fire Effects Information System (FEIS) [Online]. U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Fire Sciences Laboratory. Available from https://www.fs.fed.us/database/feis/plants/tree/robpse/all.html [accessed 1 May 2020].

Thompson J.D., Higgins D.G. & Gibson T.J. 1994. Clustal W: improving the sensitivity of progressive multiple sequence aligment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22 (22): 4673–4680. https://doi.org/10.1093/nar/22.22.4673

Thompson W.R. 1958. A Catalogue of the Parasites and Predators of Insect Pests. Commonwealth Agricultural Bureaux, Commonwealth Institute of Biological Control, Ottawa, Ontario, Canada.

Vítková M., Müllerová J., Sádlo J., Pergl J. & Pyšek P. 2017. Black locust (Robinia pseudoacacia) beloved and despised: a story of an invasive tree in Central Europe. Forest Ecology and Management 384: 287–302. https://doi.org/10.1016/j.foreco.2016.10.057

von Rosen H. 1958. Zur Kenntnis des Pteromaliden-Genus Mesopolobus Westwood, 1833 (Hym., Chalc.). Opuscula Entomologica 23 (3): 203–240.

von Rosen H. 1959. Zur Kenntnis der Pteromaliden-Genus Mesopolobus Westwood 1833 (Hym., Chalcidoidea) IV. Entomologisk Tidskrift 80 (3/4): 146–162.

von Rosen H. 1960. Zur Kenntnis des Pteromaliden-Genus Mesopolobus Westwood 1833 (Hym., Chalc.) VI. Opuscula Entomologica 25: 16–29.

von Rosen H. 1961. Zur Kenntnis der Pteromaliden-Genus Mesopolobus Westwood 1833 Hym. Chalc. Anzeiger für Schädlingskunde 34 (4): 62–62.

Westwood J.O. 1833. LXXIII. Descriptions of several new British forms amongst the parasitic hymenopterous insects. The London, Edinburgh, and Dublin Philosophical Magazine and Journal of Science Series 3 2 (12): 443–445. https://doi.org/10.1080/14786443308648084

Xiao H., Sun L., Jiao T. & Li Z. 2016. A revision of Chinese species of Mesopolobus Westwood (Hymenoptera: Pteromalidae) with descriptions of four new species from China. Zoological Systematics 41 (1): 64–81. https://doi.org/10.11865/zs.201604

Zerova M.D. 1970. A new species of the genus Bruchophagus Ashm. (Hymenoptera, Eurytomidae) from the south part of the USSR. Vestnik Zoologii 5: 77–79. [in Russian.]

How to Cite
László, Z., Lakatos, K. T., & Dénes, A.-L. (2021). A new species of Mesopolobus (Hymenoptera, Pteromalidae) from black locust crops. European Journal of Taxonomy, 740(1), 118–137. https://doi.org/10.5852/ejt.2021.740.1285
Research article