Integrative description of new giant pill-millipedes from southern Thailand (Diplopoda, Sphaerotheriida, Zephroniidae)
Abstract
Two new species of giant pill-millipedes, Zephronia viridisoma Rosenmejer & Wesener sp. nov. and Sphaerobelum aesculus Rosenmejer & Wesener sp. nov., are described based on museum samples from southern Thailand. Zephronia viridisoma sp. nov. comes from Khao Lak, while the type locality of S. aesculus sp. nov. is on Phuket Island. Both species are described integratively, combining light microscopy, scanning electron microscopy, multi-layer photography, micro-CT scans and genetic barcoding. Genetic barcoding was successfully conducted for holotypes of both new species, which could be added to a dataset of all published sequences of the family Zephroniidae, including all described species from Thailand, Laos and Cambodia up to 2020. Genetic barcoding of the COI gene revealed another female of S. aesculus sp. nov., 160 km east of the type locality. Both new species are genetically distant from all other Zephroniidae from Thailand and surrounding countries, showing uncorrected p-distances of 16.8–23.1%. A virtual cybertype of a paratype of Z. viridisoma sp. nov. was created and made publically accessible.
References
Altschul S.F., Madden T.L., Schäffner A.A., Zhang J., Zhang Z., Miller W. & Lipman D.J. 1997. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Research 25 (17): 3389–3402. https://doi.org/10.1093/nar/25.17.3389
Astrin J.J. & Stüben P.E. 2008. Phylogeny in cryptic weevils: molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera: Curculionidae). Invertebrate Systematics 22: 503–522. https://doi.org/10.1071/IS07057
Clements R., Sodhi NS., Schilthuizen M. & Ng P.K. 2006. Limestone karsts of Southeast Asia: imperiled arks of biodiversity. Bioscience 56 (9): 733–742. https://doi.org/10.1641/0006-3568(2006)56[733:LKOSAI]2.0.CO;2
Enghoff H. 2005. The millipedes of Thailand (Diplopoda). Steenstrupia 29: 87–103.
Enghoff H., Sutcharit C. & Panha S. 2007. The shocking pink dragon millipede, Desmoxytes purpurosea, a colourful new species from Thailand (Diplopoda: Polydesmida: Paradoxosomatidae). Zootaxa 1563: 31–36. https://doi.org/10.11646/zootaxa.1563.1.3
Enghoff H., Golovatch S., Short M., Stoev P. & Wesener T. 2015. Diplopoda. In: Minelli A. (ed.) Treatise on Zoology — Anatomy, Taxonomy, Biology. The Myriapoda 2 (16): 363–453.
Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hebert P.D., Cywinska A., Ball S.L. & Dewaard J.R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270 (1512): 313–321. https://doi.org/10.1098/rspb.2002.2218
Hirst A.S. 1907. On four new pill-millipedes from the Malay Peninsula and Siam. Annals and Magazine of Natural History Series 7 20: 215–219, pl. 10. https://doi.org/10.1080/00222930709487327
Jeekel C.A.W. 2000. A new genus of Sphaeropoeidae from Malaysia (Diplopoda, Sphaerotheriida). Myriapod Memoranda 2: 68–70.
Karsch F. 1881. Neue Juliden des Berliner Museums, als Prodromus einer Juliden-Monographie. Zeitschrift für die gesamten Naturwissenschaften, Dritte Folge 6: 3–79.
Likhitrakarn N., Golovatch S.I., Srisonchai R. & Panha S. 2019. A new species of the millipede genus Cryptocorypha Attems, 1907, from northern Thailand (Polydesmida, Pyrgodesmidae). ZooKeys 833: 121–132. https://doi.org/10.3897/zookeys.833.32413
Likhitrakarn N., Golovatch S.I., Srisonchai R. & Sutcharit C. 2021. Two new species of the giant pill-millipede genus Zephronia Gray, 1832 from Thailand (Diplopoda: Sphaerotheriida: Zephroniidae). Tropical Natural History 21: 12–26.
Limaye A. 2012. Drishti: a volume exploration and presentation tool. In: Developments in X-ray Tomography VIII. Proceedings SPIE 8506: 85060X. International Society for Optics and Photonics. https://doi.org/10.1117/12.935640
Mauriès J.-P. 2001. Sur l’identité de Zephronia hainani Gressit, 1941, à propos de la description d’un nouveau Prionobelum (Diplopoda, Sphaerotheriida, Sphaeropoeidae) de Hainan, Chine. Zoosystema 23 (1): 131–142.
Moritz L. & Wesener T. 2017. Integrative description of two new species of Malagasy chirping giant pill-millipedes, genus Sphaeromimus (Diplopoda, Sphaerotheriida, Arthrosphaeridae). European Journal of Taxonomy 381: 1–25. https://doi.org/10.5852/ejt.2017.381
Nei M. & Kumar S. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Pimvichai P., Enghoff H., Panha S. & Backeljau T. 2018. Morphological and mitochondrial DNA data reshuffle the taxonomy of the genera Atopochetus Attems, Litostrophus Chamberlin and Tonkinbolus Verhoeff (Diplopoda: Spirobolida: Pachybolidae), with descriptions of nine new species. Invertebrate Taxonomy 32: 159–195. https://doi.org/10.1071/IS17052
Pimvichai P., Enghoff H., Panha S. & Backeljau T. 2020. Integrative taxonomy of the new millipede genus Coxobolellus, gen. nov. (Diplopoda: Spirobolida: Pseudospirobolellidae), with descriptions of ten new species. Invertebrate Systematics 34 (6): 591–617. https://doi.org/10.1071/IS20031
QGIS Development Team 2018. QGIS Geographic Information System. Open Source Geospatial Foundation Project. Available from http://qgis.osgeo.org [accessed 27 Jul. 2021].
Reip H.S. & Wesener T. 2018. Intraspecific variation and phylogeography of the millipede model organism, the black pill millipede Glomeris marginata (Villers, 1789) (Diplopoda, Glomerida, Glomeridae). In: Stoev P. & Edgecombe G.D. (eds) Proceedings of the 17th International Congress of Myriapodology, Krabi, Thailand. ZooKeys 741: 93–131. https://doi.org/10.3897/zookeys.741.21917
Sagorny C. & Wesener T. 2017. Two new giant pill-millipede species of the genus Zoosphaerium endemic to the Bemanevika area in northern Madagascar (Diplopoda, Sphaerotheriida, Arthrosphaeridae). Zootaxa 4263 (2): 273–294.
Schindelin J., Arganda-Carreras I., Frise E., Kaynig V., Longair M., Pietzsch T., Preibisch S., Rueden C., Saalfeld S., Schmid B., Tinevez J.-Y., White D.J., Hartenstein V., Eliceiri K., Tomancak P. & Cardona A. 2012. Fiji: an open-source platform for biological-image analysis. Nature methods 9 (7): 676–682. https://doi.org/10.1038/nmeth.2019
Semenyuk I., Golovatch S.I. & Wesener T. 2018. Four new species of giant pill-millipedes from Vietnam (Sphaerotheriida: Zephroniidae). Zootaxa 4459 (3): 535–550. https://doi.org/10.11646/zootaxa.4459.3.7
Semenyuk I., Golovatch S.I. & Wesener T. 2020. Some new or poorly-known Zephroniidae (Diplopoda, Sphaerotheriida) from Vietnam. ZooKeys 930: 37–60. https://doi.org/10.3897/zookeys.930.47742
Srisonchai R., Enghoff H., Likhitrakarn N., Sutcharit C. & Panha S. 2016. Four colourful new species of dragon millipedes, genus Desmoxytes Chamberlin, 1923, from northern Thailand (Diplopoda: Polydesmida: Paradoxosomatidae). Zootaxa 4170 (1): 93–113. https://doi.org/10.11646/zootaxa.4170.1.4
Tamura K., Stecher G., Peterson D., Filipski A. & Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197
Wesener T. 2016a. The giant pill-millipedes, order Sphaerotheriida – An annotated species catalogue with morphological atlas and list of apomorphies (Arthropoda: Diplopoda). Bonn Zoological Bulletin - Supplementum 63: 1–104.
Wesener T. 2016b. Redescription and phylogenetic analysis of the type species of the giant pill-millipede genus Sphaeropoeus Brandt, 1833 (Diplopoda, Sphaerotheriida, Zephroniidae). Zootaxa 4184 (1): 141–157. https://doi.org/10.11646/zootaxa.4184.1.9
Wesener T. 2019. First records of giant pill-millipedes from Laos (Diplopoda, Sphaerotheriida, Zephroniidae). Zootaxa 4563 (2): 201–248. https://doi.org/10.11646/zootaxa.4563.2.1
Wesener T. & Anilkumar P.A. 2020. The millipedes collected by the Museum “La Specola” on Madagascar 1989/1991, with the description of three new species of giant pill-millipedes (Diplopoda, Sphaerotheriida, Arthrosphaeridae). ZooKeys 930: 3–35. https://doi.org/10.3897/zookeys.930.47620
Wesener T. & Conrad C. 2016. Local hotspots of endemism or artifacts of incorrect taxonomy? The status of microendemic pill millipede species of the genus Glomeris in Northern Italy (Diplopoda, Glomerida). PLoS ONE 11 (9): e0162284. https://doi.org/10.1371/journal.pone.0162284
Wesener T. & Sagorny C. 2021. Seven new giant pill-millipede species and numerous new records of the genus Zoosphaerium from Madagascar (Diplopoda, Sphaerotheriida, Arthrosphaeridae). European Journal of Taxonomy 758 (1): 1–48. https://doi.org/10.5852/ejt.2021.758.1423
Wesener T., Minh-Tu Le D. & Loria S.F. 2014. Integrative revision of the giant pill-millipede genus Sphaeromimus, with the description of seven new species (Diplopoda, Sphaerotheriida, Arthrosphaeridae). ZooKeys 414: 67–104. https://doi.org/10.3897/zookeys.414.7730
Wesener T., Wongthamwanich N. & Moritz L. 2021. Description of the first species of Glomeridesmida from Thailand (Diplopoda, Glomeridesmida, Glomeridesmidae). Zookeys 1024: 137–156. https://doi.org/10.3897/zookeys.1024.63678
Wongthamwanich N., Panha, S. Sierwald, P. Wesener T. & Thirakhupt K. 2012. A new species of the giant pill-millipede genus Sphaerobelum Verhoeff, 1924 from northern Thailand, with an extensive description and molecular characters (Diplopoda: Sphaerotheriida: Zephroniidae). Zootaxa 3220: 29–43. https://doi.org/10.11646/zootaxa.3220.1.2
Wongthamwanich N., Panha S., Sitthicharoenchai D., Pradatsundarasar A., Seelanan T., Enghoff H. & Thirakhupt K. 2013 (for 2012). Daily activities of the giant pill-millipede Zephronia cf. viridescens Attems, 1936 (Diplopoda: Sphaerotheriida: Zephroniidae) in a deciduous forest in Northern Thailand. Zoological Studies 51: 913–926.
Zhao Y., Yu J., & Liu W. 2020. A molecular-based phylogeny of the millipede genus Sphaerobelum Verhoeff, 1924, with the first record of the genus from mainland China (Diplopoda: Sphaerotheriida: Zephroniidae). Annales de la Société entomologique de France (NS) 56 (4): 341–348.
Copyright (c) 2021 Trine Rosenmejer, Henrik Enghoff, Leif Moritz, Thomas Wesener
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