Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species
Abstract
Despite several decades of active research, there are still substantial gaps in the knowledge of parasitoid wasps in Australia, with many families and genera yet to be revised using modern approaches and only a fraction of the estimated fauna currently described. The genus Glyptapanteles Ashmead, 1904 is a member of the subfamily Microgastrinae (Hymenoptera: Braconidae) and all species in the subfamily are lepidopteran parasitoids. The genus previously contained only three species known from Australia: G. deliasa Austin & Dangerfield, 1992, G. drioplanetus Fagan-Jeffries & Austin, 2021 and G. mnesampela Austin, 2000. To undertake a revision of this morphologically-conserved group in Australia, we used a combination of molecular (cytochrome oxidase subunit one (COI) and wingless genes) and minimal morphological data to delimit and describe an additional 31 species: G. austini Fagan-Jeffries & Bird sp. nov. and the following 30 species all authored by Fagan-Jeffries, Bird & Austin: G. albigena sp. nov., G. andamookaensis sp. nov., G. arcanus sp. nov., G. aspersus sp. nov., G. austrinus sp. nov., G. baylessi sp. nov., G. bradfordae sp. nov., G. cooperi sp. nov., G. doreyi sp. nov., G. dowtoni sp. nov., G. eburneus sp. nov., G. erucadesolator sp. nov., G. ferrugineus sp. nov., G. foraminous sp. nov., G. goodwinnoakes sp. nov., G. guzikae sp. nov., G. harveyi sp. nov., G. kingae sp. nov., G. kittelae sp. nov., G. kurandaensis sp. nov., G. lambkinae sp. nov., G. lessardi sp. nov., G. mouldsi sp. nov., G. niveus sp. nov., G. rixi sp. nov., G. rodriguezae sp. nov., G. ruhri sp. nov., G. sanniopolus sp. nov., G. vergrandiacus sp. nov. and G. wrightae sp. nov. We provide a key to species groups and to the species able to be identified on morphological characters alone. Additionally, we provide a brief discussion of the difficulties in describing small, morphologically conserved wasps and the challenges associated with revising the taxonomy of hyperdiverse taxa in the context of the planned mission of Taxonomy Australia to accelerate the documentation of Australia’s biodiversity.
References
Ahrens D., Ahyong S.T., Ballerio A., Barclay M.V.L., Eberle J., Espeland M., Huber B.A., Mengual X., Pacheco T.L., Peters R.S., Rulik B., Vaz-de-Mello F., Wesener T. & Krell F.-T. 2021. Is it time to describe new species without diagnoses? – A comment on Sharkey et al. (2021). Zootaxa 5027 (2): 151–159. https://doi.org/10.11646/zootaxa.5027.2.1
Arias-Penna D.C., Whitfield J.B., Janzen D.H., Hallwachs W., Dyer L.A., Smith M.A., Hebert P.D.N. & Fernández-Triana J.L. 2019. A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador. ZooKeys 890: 1–685. https://doi.org/10.3897/zookeys.890.35786
Austin A.D. & Dangerfield P.C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1–76. https://doi.org/10.1071/IT9920001
de Queiroz K. 1998. The general lineage concept of species, species criteria and the process of speciation. In: Endless Forms: Species and Speciation: 57–75. Oxford University Press, Oxford.
Eady R.D. 1968. Some illustrations of microsculpture in the Hymenoptera. Proceedings of the Royal Entomological Society of London. Series A, General Entomology 43: 66–72. https://doi.org/10.1111/j.1365-3032.1968.tb01029.x
Fagan-Jeffries E.P. & Austin A.D. 2020. Synopsis of the parasitoid wasp genus Cotesia Cameron, 1891 (Hymenoptera: Braconidae: Microgastrinae) in Australia, with the description of seven new species. European Journal of Taxonomy 667: 1–70. https://doi.org/https://doi.org/10.5852/ejt.2020.667
Fagan-Jeffries E.P. & Austin A.D. 2021. Four new species of parasitoid wasp (Hymenoptera: Braconidae) described through a citizen science partnership with schools in regional South Australia. Zootaxa 4949 (1): 79–101. https://doi.org/https://doi.org/10.11646/zootaxa.4949.1.4
Fagan-Jeffries E.P., Cooper S.J.B., Bertozzi T., Bradford T.M. & Austin A.D. 2018. DNA barcoding of microgastrine parasitoid wasps (Hymenoptera: Braconidae) using high-throughput methods more than doubles the number of species known for Australia. Molecular Ecology Resources 18: 1132–1143. https://doi.org/10.1111/1755-0998.12904
Fagan-Jeffries E.P., Cooper S.J.B. & Austin A.D. 2019. New species of Australian microgastrine parasitoid wasps (Hymenoptera: Braconidae: Microgastrinae) documented through the ‘Bush Blitz’ surveys of national reserves. Zootaxa 4560 (3): 401–440. https://doi.org/10.11646/zootaxa.4560.3.1
Fernández-Triana J., Shaw M.R., Boudreault C., Beaudin M. & Broad G.R. 2020. Annotated and illustrated world checklist of Microgastrinae parasitoid wasps (Hymenoptera, Braconidae). ZooKeys 920: 1–1089. https://doi.org/10.3897/zookeys.920.39128
Fullaway D.T. 1957. Checklist of the Hymenoptera of Fiji. Proceedings of the Hawaiian Entomological Society 16: 269–280.
Gupta A., Venkatesan T. & More R.P. 2016. Morphological and molecular characterization of reared parasitoid wasps of the genus Glyptapanteles Ashmead 1904 (Insecta: Hymenoptera: Braconidae: Microgastrinae) associated with Lepidoptera in India. PLoS ONE 11: e0150765. https://doi.org/10.1371/journal.pone.0150765
Hoang D.T., Chernomor O., Von Haeseler A., Minh B.Q. & Vinh L.S. 2018. UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35: 518–522. https://doi.org/10.1093/molbev/msx281
Kalyaanamoorthy S., Minh B.Q., Wong T.K.F., Von Haeseler A. & Jermiin L.S. 2017. ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
Katoh K. & Standley D.M. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
Katoh K., Misawa K., Kuma K.I. & Miyata T. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30 (14): 3059–3066. https://doi.org/10.1093/nar/gkf436
Mason W.R.M. 1981. The polyphyletic nature of Apanteles Foerster (Hymenopera: Braconidae): a phylogeny and reclassification of Microgastrinae. Memoirs of the Entomological Society of Canada 113: 1–147. https://doi.org/10.4039/entm113115fv
Meier R., Blaimer B., Buenaventura E., Hartop E., Rintelen T. von, Srivathsan A. & Yeo D. 2021. A re-analysis of the data in Sharkey et al.’s (2021) minimalist revision reveals that BINs do not deserve names, but BOLD Systems needs a stronger commitment to open science. Cladistics 2021: 1–12. https://doi.org/10.1111/cla.12489
Nguyen L.T., Schmidt H.A., Von Haeseler A. & Minh B.Q. 2015. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
Ratnasingham S. & Hebert P.D.N. 2013. A DNA-based registry for all animal species: the Barcode Index Number (BIN) system. PLoS ONE 8: e66213. https://doi.org/10.1371/journal.pone.0066213
Rodriguez J.J., Fernández-Triana J.L., Smith M.A., Janzen D.H., Hallwachs W., Erwin T.L. & Whitfield J.B.. 2013. Extrapolations from field studies and known faunas converge on dramatically increased estimates of global microgastrine parasitoid wasp species richness (Hymenoptera: Braconidae). Insect Conservation and Diversity 6: 530–536. https://doi.org/10.1111/icad.12003
Sharkey M.J., Janzen D.H., Hallwachs W., Chapman, E.G., Smith M.A., Dapkey T., Brown A., Ratnasingham S., Naik S., Manjunath R., Perez K., Milton M., Hebert P., Shaw S.R., Kittel R.N., Solis M.A., Metz M.A., Goldstein P.Z., Brown J.W., Quicke D.L.J., van Achterberg C., Brown B.V. & Burns J.M. 2021. Minimalist revision and description of 403 new species in 11 subfamilies of Costa Rican braconid parasitoid wasps, including host records for 219 species. ZooKeys 2021: 1–665. https://doi.org/10.3897/zookeys.1013.55600
Shenefelt R.D. 1972. Braconidae 4, Microgasterinae, Apanteles. In: van der Vecht J. & Shenefelt R.D. (eds) Hymenopterorum Catalogus Part 7: 429–668. W. Junk. Gravenhage.
Taxonomy Decadal Plan Working Group 2018. Discovering Biodiversity: A Decadal Plan for Taxonomy and Biosystematics in Australia and New Zealand 2018–2028. Canberra and Wellington. Available from https://www.science.org.au/ [accessed 4 Jan. 2022].
Wilkinson D.S. 1928. A revision of the Indo-Australian species of the genus Apanteles (Hym. Bracon.). Part II. Bulletin of Entomological Research 19: 109–146. https://doi.org/10.1017/S0007485300020393
Yeates D.K., Harvey M.S. & Austin A.D. 2003. New estimates for terrestrial arthropod species-richness in Australia. Records of the South Australian Museum, Monograph Series 7: 231–241.
Copyright (c) 2022 Erinn P. Fagan-Jeffries, Alana R. McClelland, Andrew J. Bird, Madalene M. Giannotta, Tessa M. Bradford, Andrew D. Austin
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