Taxonomic revaluation of the Ahaetulla prasina (H. Boie in F. Boie, 1827) complex from Northeast India: resurrection and redescription of Ahaetulla flavescens (Wall, 1910) (Reptilia: Serpentes: Colubridae)
Abstract
The taxonomic status of the nominal taxon Dryophis prasinus flavescens Wall, 1910 is reevaluated herein. Based on molecular data generated from fresh collections of Ahaetulla prasina (H. Boie in F. Boie, 1827) auctorum from Northeast India and, additionally, morphological data from museum specimens originating from the same areas, we resurrect this taxon as Ahaetulla flavescens (Wall, 1910) comb. nov. We clarify the status, identity and locations of its type specimens, rediscover, redescribe and illustrate those specimens and also designate a lectotype in order to effect a proper taxonomic redefinition of this nominal taxon. We provide further details on the morphology and diagnosis of this species and elucidate its phylogenetic position. We also provide a summary of the natural history and distribution of this species. Adding to the known cryptic diversity and genetic divergence within Southeast Asian populations, this work also hints at the need for a taxonomic revision of the A. prasina complex. This work complements a previous study on the A. prasina complex focusing on populations in Indonesia. Taken together, these two studies represent phylogenetic reconstructions from different populations of the A. prasina complex across its distribution range, on the Asian mainland and the surrounding islands.
References
Ahmed F., Das A. & Dutta S.K. 2009. Amphibians and Reptiles of Northeast India: A Photographic Guide. Aaranyak, Guwahati, India.
Arévalo E., Davis S.K. & Sites J.W. 1994. Mitochondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in Central Mexico. Systematic Biology 43 (3): 387–418. https://doi.org/10.1093/sysbio/43.3.387
Boie F. 1827. Bemerkungen über Merrem’s Versuch eines Systems der Amphibien, 1. Lieferung: Ophidier. Isis von Oken 20 (6): col. 508–566. Available from https://www.biodiversitylibrary.org/page/26453570 [accessed 24 Aug. 2022].
Boulenger G.A. 1890. The Fauna of British India, including Ceylon and Burma. Reptilia and Batrachia. Taylor & Francis, London, UK. https://doi.org/10.5962/bhl.title.5490
Boulenger G.A. 1896. Catalogue of the Snakes in the British Museum (Natural History). Vol. III, Containing the Colubridae (Opisthoglyphae and Proteroglyphae), Amblycephalidae and Viperidae. British Museum (Natural History), London, UK. https://doi.org/10.5962/bhl.title.8316
Burbrink F.T., Lawson R. & Slowinski J.B. 2000. Mitochondrial DNA phylogeography of the polytypic North American rat snake (Elaphe obsoleta): a critique of the subspecies concept. Evolution 54 (6): 2107–2118. https://doi.org/10.1111/j.0014-3820.2000.tb01253.x
Cantor T.E. 1847. Catalogue of reptiles inhabiting the Malay Peninsula and the Islands. Journal of the Asiatic Society of Bengal 16 (2): 897–952. https://doi.org/10.5962/bhl.title.5057
Das I. 2002. Photographic Guide to Snakes and other Reptiles of India. New Holland Publishers, UK.
Das I. & Chaturvedi N. 1998. Catalogue of the herpetological types in the collection of the Bombay Natural History Society. Hamadryad 23 (2): 150–156.
Deepak V., Narayanan S., Sarkar V., Dutta S.K. & Mohapatra P.P. 2019. A new species of Ahaetulla Link, 1807 (Serpentes: Colubridae: Ahaetullinae) from India. Journal of Natural History 53 (9–10): 497–516. https://doi.org/10.1080/00222933.2019.1589591
Dowling H.G. 1951. A proposed standard system of counting ventrals in snakes. British Journal of Herpetology 1 (5): 97–99.
Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32 (5): 1792–1797. https://doi.org/10.1093/nar/gkh340
Figueroa A., McKelvy A.D., Grismer L.L., Bell C.D. & Lailvaux S.P. 2016. A species-level phylogeny of extant snakes with description of a new colubrid subfamily and genus. PloS ONE 11 (9): e0161070. https://doi.org/10.1371/journal.pone.0161070
Gaulke M. 1994. Eine neue Unterart des Malaysischen Baumschnüfflers, Ahaetulla prasina suluensis n. subsp. (Reptilia: Serpentes: Colubridae). Senckenbergiana Biologica 73 (1–2): 45–47.
Günther A.C.L.G. 1858. Catalogue of Colubrine Snakes in the Collection of the British Museum, Vol. 4. Printed by order of Trustees, London, UK. https://doi.org/10.5962/bhl.title.13272
Günther A.C.L.G. 1864. The Reptiles of British India. The Ray Society, London, UK. https://doi.org/10.5962/bhl.title.5012
Gutell R.R. 1994. Collection of small subunit (16S-and 16S-like) ribosomal RNA structures: 1994. Nucleic Acids Research 22 (17): 3502–3507. https://doi.org/10.1093/nar/22.17.3502
Hoang D.T., Chernomor O., Von Haeseler A., Minh B.Q. & Vinh L.S. 2018. UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35 (2): 518–522. https://doi.org/10.1093/molbev/msx281
ICZN (International Commission on Zoological Nomenclature). 1999. International Code of Zoological Nomenclature, Fourth edition. The Natural History Museum, London, UK. Available from https://www.iczn.org/the-code/the-code-online [accessed 24 Aug. 2022].
Indahsari L.I.N., Fatchiyah F., Smith E.N. & Kurniawan N. 2020. First record of Ahaetulla mycterizans (Linnaeus, 1758) (Serpentes: Colubridae) from the Lesser Sunda region, Indonesia, based on molecular and morphological identification. Turkish Journal of Zoology 44 (1): 11–21. https://doi.org/10.3906/zoo-1904-1
Kjer K.M. 1995. Use of rRNA secondary structure in phylogenetic studies to identify homologous positions: an example of alignment and data presentation from the frogs. Molecular Phylogenetics and Evolution 4 (3): 314–330. https://doi.org/10.1006/mpev.1995.1028
Lam N.Q., Thu T.T.A., Nguyen L.T., Murphy R.W. & Nguyen S.N. 2021. A new species of Ahaetulla Link, 1807 (Squamata: Colubridae) from the Mekong Delta, Vietnam. Zootaxa 4966 (3): 290–304. https://doi.org/10.11646/zootaxa.4966.3.2
Lanfear R., Calcott B., Ho S.Y. & Guindon S. 2012. PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29 (6): 1695–1701. https://doi.org/10.1093/molbev/mss020
Lazell J.D. 2002. The herpetofauna of Shek Kwu Chau, South Chinese Sea, with descriptions of two new colubrid snakes. Memoirs of the Hong Kong Natural History Society 25: 1–82.
Mallik A.K., Achyuthan N.S., Ganesh S.R., Pal S.P., Vijayakumar S.P. & Shanker K. 2019. Discovery of a deeply divergent new lineage of vine snake (Colubridae: Ahaetuliinae: Proahaetulla gen. nov.) from the southern Western Ghats of Peninsular India with a revised key for Ahaetuliinae. PLoS ONE 14 (7): e0218851. https://doi.org/10.1371/journal.pone.0218851
Mallik A.K., Srikanthan A.N., Pal S.P., D’Souza P.M., Shanker K. & Ganesh S.R. 2020. Disentangling vines: a study of morphological crypsis and genetic divergence in vine snakes (Squamata: Colubridae: Ahaetulla) with the description of five new species from Peninsular India. Zootaxa 4874 (1): 1–62. https://doi.org/10.11646/zootaxa.4874.1.1
Mell R. 1931. List of Chinese snakes. Lingnan Science Journal 8 [1929]: 199–219.
Minh B.Q., Schmidt H.A., Chernomor O., Schrempf D., Woodhams M.D., Haeseler A. von & Lanfea R. 2020. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution 37 (5): 1530–1534. https://doi.org/10.1093/molbev/msaa015
Mohapatra P.P., Dutta S.K., Kar N.B., Das A., Murthy B.H.C.K & Deepak V. 2017. Ahaetulla nasuta anomala (Annandale, 1906) (Squamata: Colubridae), resurrected as a valid species with marked sexual dichromatism. Zootaxa 4263 (2): 318–332. https://doi.org/10.11646/ZOOTAXA.4263.2.6
Palumbi S.R. 1996. Nucleic acids II: the polymerase chain reaction. In: Hillis D.M., Moritz C. & Mable B.K. (eds) Molecular Systematics, 2nd edition: 205–247. Sinauer, Sunderland, MA.
Parkinson C.L. 1999. Molecular systematics and biogeographical history of pitvipers as determined by mitochondrial ribosomal DNA sequences. Copeia 1999: 576–586. https://doi.org/10.2307/1447591
Sambrook J. 2001. Preparation and analysis of eukaryotic genome DNA. Molecular Cloning: A Laboratory Manual 1: 6–1.
Sclater W.L. 1891. List of Snakes in the Indian Museum. Printed by order of Trustees of Indian Museum, The Indian Museum, Calcutta, India. https://doi.org/10.5962/bhl.title.8746
Smith M.A. 1943. The Fauna of British India, Ceylon and Burma, including the whole of the Indo-Chinese Sub-Region. Reptilia and Amphibia, Vol. 3 (Serpentes). Taylor and Francis, London.
Stecher G., Tamura K. & Kumar S. 2018. Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Molecular Biology and Evolution 37 (4): 1237–1239. https://doi.org/10.1093/molbev/msz312
Taylor E.H. 1922. The Snakes of the Philippine Islands. Dalcassian Publishing Company, USA.
Theobald W. 1876. Descriptive Catalogue of the Reptiles of British India. The Indian Museum, Calcutta. https://doi.org/10.5962/bhl.title.5483
Trifinopoulos J., Nguyen L.T., von Haeseler A. & Minh B.Q. 2016. Nucleic Acids Research 44 (W1): W232–W235. https://doi.org/10.1093/nar/gkw256
Uetz P., Hallermann J. & Hosek J. 2021. EMBL Reptile Database. Genus: Ahaetulla. Available from https://reptiledatabase.reptarium.cz/ [accessed 1 Jul. 2022].
Wall F. 1906. A popular treatise on the common Indian snakes. Journal of the Bombay Natural History Society 17 (1): 1–9. https://www.biodiversitylibrary.org/part/24098
Wall F. 1909a. Notes on snakes from the neighbourhood of Darjeeling. Journal of the Bombay Natural History Society 19 (2): 337–357. https://www.biodiversitylibrary.org/part/33733
Wall F. 1909b. Notes on snakes collected in the Jalpaiguri district. Journal of the Bombay Natural History Society 19 (3): 897–700. Available from https://www.biodiversitylibrary.org/part/220519 [accessed 1 Jul. 2022].
Wall F. 1910. Notes on snakes collected in upper Assam. Part II. Journal of the Bombay Natural History Society 19: 825–845. https://www.biodiversitylibrary.org/item/28012#page/939/mode/1up
Wallach V., Williams K.L. & Boundy J. 2014. Snakes of the World: A Catalogue of Living and Extinct Species. Taylor and Francis, CRC Press.
Whitaker R. & Captain A. 2004. Snakes of India – The Field Guide. Draco Books, India.
Zhang J., Kapli P., Pavlidis P. & Stamatakis A. 2013. A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29 (22): 2869–2876. https://doi.org/10.1093/bioinformatics/btt499
Copyright (c) 2022 Achyuthan N. Srikanthan, Omkar Dilip Adhikari, Ashok Kumar Mallik, Patrick D. Campbell, Bharat Bhushan Bhatt, Kartik Shanker, Sumaithangi Rajagopalan Ganesh
This work is licensed under a Creative Commons Attribution 4.0 International License.
Creative Commons Copyright Notices
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are NOT ALLOWED TO post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to taxonomic issues.