Taxonomic revaluation of the Ahaetulla prasina (H. Boie in F. Boie, 1827) complex from Northeast India: resurrection and redescription of Ahaetulla flavescens (Wall, 1910) (Reptilia: Serpentes: Colubridae)

  • Achyuthan N. Srikanthan Centre for Ecological Sciences, Indian Institute of Sciences, Bangalore – 560012, Karnataka, India
  • Omkar Dilip Adhikari Natural History collection department, Bombay Natural History Society, Hornbill House, Opp. Lion Gate, S.B.S. Road, Mumbai – 400001, Maharashtra, India
  • Ashok Kumar Mallik Centre for Ecological Sciences, Indian Institute of Sciences, Bangalore – 560012, Karnataka, India
  • Patrick D. Campbell Department of Life Sciences, Darwin Centre, Natural History Museum, Cromwell Road, South Kensington, London SW7 5BD, England
  • Bharat Bhushan Bhatt Department of Environment & Forest, P-Sector, Itanagar – 791111, Arunachal Pradesh, India
  • Kartik Shanker Centre for Ecological Sciences, Indian Institute of Sciences, Bangalore – 560012, Karnataka, India
  • Sumaithangi Rajagopalan Ganesh Chennai Snake Park, Rajbhavan post, Chennai – 600 022, Tamil Nadu, India
Keywords: Assam, Arunachal Pradesh, BNHS, DNA, distribution, scalation, vine snakes

Abstract

The taxonomic status of the nominal taxon Dryophis prasinus flavescens Wall, 1910 is reevaluated herein. Based on molecular data generated from fresh collections of Ahaetulla prasina (H. Boie in F. Boie, 1827) auctorum from Northeast India and, additionally, morphological data from museum specimens originating from the same areas, we resurrect this taxon as Ahaetulla flavescens (Wall, 1910) comb. nov. We clarify the status, identity and locations of its type specimens, rediscover, redescribe and illustrate those specimens and also designate a lectotype in order to effect a proper taxonomic redefinition of this nominal taxon. We provide further details on the morphology and diagnosis of this species and elucidate its phylogenetic position. We also provide a summary of the natural history and distribution of this species. Adding to the known cryptic diversity and genetic divergence within Southeast Asian populations, this work also hints at the need for a taxonomic revision of the A. prasina complex. This work complements a previous study on the A. prasina complex focusing on populations in Indonesia. Taken together, these two studies represent phylogenetic reconstructions from different populations of the A. prasina complex across its distribution range, on the Asian mainland and the surrounding islands.

References

Ahmed F., Das A. & Dutta S.K. 2009. Amphibians and Reptiles of Northeast India: A Photographic Guide. Aaranyak, Guwahati, India.

Arévalo E., Davis S.K. & Sites J.W. 1994. Mitochondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in Central Mexico. Systematic Biology 43 (3): 387–418. https://doi.org/10.1093/sysbio/43.3.387

Boie F. 1827. Bemerkungen über Merrem’s Versuch eines Systems der Amphibien, 1. Lieferung: Ophidier. Isis von Oken 20 (6): col. 508–566. Available from https://www.biodiversitylibrary.org/page/26453570 [accessed 24 Aug. 2022].

Boulenger G.A. 1890. The Fauna of British India, including Ceylon and Burma. Reptilia and Batrachia. Taylor & Francis, London, UK. https://doi.org/10.5962/bhl.title.5490

Boulenger G.A. 1896. Catalogue of the Snakes in the British Museum (Natural History). Vol. III, Containing the Colubridae (Opisthoglyphae and Proteroglyphae), Amblycephalidae and Viperidae. British Museum (Natural History), London, UK. https://doi.org/10.5962/bhl.title.8316

Burbrink F.T., Lawson R. & Slowinski J.B. 2000. Mitochondrial DNA phylogeography of the polytypic North American rat snake (Elaphe obsoleta): a critique of the subspecies concept. Evolution 54 (6): 2107–2118. https://doi.org/10.1111/j.0014-3820.2000.tb01253.x

Cantor T.E. 1847. Catalogue of reptiles inhabiting the Malay Peninsula and the Islands. Journal of the Asiatic Society of Bengal 16 (2): 897–952. https://doi.org/10.5962/bhl.title.5057

Das I. 2002. Photographic Guide to Snakes and other Reptiles of India. New Holland Publishers, UK.

Das I. & Chaturvedi N. 1998. Catalogue of the herpetological types in the collection of the Bombay Natural History Society. Hamadryad 23 (2): 150–156.

Deepak V., Narayanan S., Sarkar V., Dutta S.K. & Mohapatra P.P. 2019. A new species of Ahaetulla Link, 1807 (Serpentes: Colubridae: Ahaetullinae) from India. Journal of Natural History 53 (9–10): 497–516. https://doi.org/10.1080/00222933.2019.1589591

Dowling H.G. 1951. A proposed standard system of counting ventrals in snakes. British Journal of Herpetology 1 (5): 97–99.

Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32 (5): 1792–1797. https://doi.org/10.1093/nar/gkh340

Figueroa A., McKelvy A.D., Grismer L.L., Bell C.D. & Lailvaux S.P. 2016. A species-level phylogeny of extant snakes with description of a new colubrid subfamily and genus. PloS ONE 11 (9): e0161070. https://doi.org/10.1371/journal.pone.0161070

Gaulke M. 1994. Eine neue Unterart des Malaysischen Baumschnüfflers, Ahaetulla prasina suluensis n. subsp. (Reptilia: Serpentes: Colubridae). Senckenbergiana Biologica 73 (1–2): 45–47.

Günther A.C.L.G. 1858. Catalogue of Colubrine Snakes in the Collection of the British Museum, Vol. 4. Printed by order of Trustees, London, UK. https://doi.org/10.5962/bhl.title.13272

Günther A.C.L.G. 1864. The Reptiles of British India. The Ray Society, London, UK. https://doi.org/10.5962/bhl.title.5012

Gutell R.R. 1994. Collection of small subunit (16S-and 16S-like) ribosomal RNA structures: 1994. Nucleic Acids Research 22 (17): 3502–3507. https://doi.org/10.1093/nar/22.17.3502

Hoang D.T., Chernomor O., Von Haeseler A., Minh B.Q. & Vinh L.S. 2018. UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35 (2): 518–522. https://doi.org/10.1093/molbev/msx281

ICZN (International Commission on Zoological Nomenclature). 1999. International Code of Zoological Nomenclature, Fourth edition. The Natural History Museum, London, UK. Available from https://www.iczn.org/the-code/the-code-online [accessed 24 Aug. 2022].

Indahsari L.I.N., Fatchiyah F., Smith E.N. & Kurniawan N. 2020. First record of Ahaetulla mycterizans (Linnaeus, 1758) (Serpentes: Colubridae) from the Lesser Sunda region, Indonesia, based on molecular and morphological identification. Turkish Journal of Zoology 44 (1): 11–21. https://doi.org/10.3906/zoo-1904-1

Kjer K.M. 1995. Use of rRNA secondary structure in phylogenetic studies to identify homologous positions: an example of alignment and data presentation from the frogs. Molecular Phylogenetics and Evolution 4 (3): 314–330. https://doi.org/10.1006/mpev.1995.1028

Lam N.Q., Thu T.T.A., Nguyen L.T., Murphy R.W. & Nguyen S.N. 2021. A new species of Ahaetulla Link, 1807 (Squamata: Colubridae) from the Mekong Delta, Vietnam. Zootaxa 4966 (3): 290–304. https://doi.org/10.11646/zootaxa.4966.3.2

Lanfear R., Calcott B., Ho S.Y. & Guindon S. 2012. PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29 (6): 1695–1701. https://doi.org/10.1093/molbev/mss020

Lazell J.D. 2002. The herpetofauna of Shek Kwu Chau, South Chinese Sea, with descriptions of two new colubrid snakes. Memoirs of the Hong Kong Natural History Society 25: 1–82.

Mallik A.K., Achyuthan N.S., Ganesh S.R., Pal S.P., Vijayakumar S.P. & Shanker K. 2019. Discovery of a deeply divergent new lineage of vine snake (Colubridae: Ahaetuliinae: Proahaetulla gen. nov.) from the southern Western Ghats of Peninsular India with a revised key for Ahaetuliinae. PLoS ONE 14 (7): e0218851. https://doi.org/10.1371/journal.pone.0218851

Mallik A.K., Srikanthan A.N., Pal S.P., D’Souza P.M., Shanker K. & Ganesh S.R. 2020. Disentangling vines: a study of morphological crypsis and genetic divergence in vine snakes (Squamata: Colubridae: Ahaetulla) with the description of five new species from Peninsular India. Zootaxa 4874 (1): 1–62. https://doi.org/10.11646/zootaxa.4874.1.1

Mell R. 1931. List of Chinese snakes. Lingnan Science Journal 8 [1929]: 199–219.

Minh B.Q., Schmidt H.A., Chernomor O., Schrempf D., Woodhams M.D., Haeseler A. von & Lanfea R. 2020. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution 37 (5): 1530–1534. https://doi.org/10.1093/molbev/msaa015

Mohapatra P.P., Dutta S.K., Kar N.B., Das A., Murthy B.H.C.K & Deepak V. 2017. Ahaetulla nasuta anomala (Annandale, 1906) (Squamata: Colubridae), resurrected as a valid species with marked sexual dichromatism. Zootaxa 4263 (2): 318–332. https://doi.org/10.11646/ZOOTAXA.4263.2.6

Palumbi S.R. 1996. Nucleic acids II: the polymerase chain reaction. In: Hillis D.M., Moritz C. & Mable B.K. (eds) Molecular Systematics, 2nd edition: 205–247. Sinauer, Sunderland, MA.

Parkinson C.L. 1999. Molecular systematics and biogeographical history of pitvipers as determined by mitochondrial ribosomal DNA sequences. Copeia 1999: 576–586. https://doi.org/10.2307/1447591

Sambrook J. 2001. Preparation and analysis of eukaryotic genome DNA. Molecular Cloning: A Laboratory Manual 1: 6–1.

Sclater W.L. 1891. List of Snakes in the Indian Museum. Printed by order of Trustees of Indian Museum, The Indian Museum, Calcutta, India. https://doi.org/10.5962/bhl.title.8746

Smith M.A. 1943. The Fauna of British India, Ceylon and Burma, including the whole of the Indo-Chinese Sub-Region. Reptilia and Amphibia, Vol. 3 (Serpentes). Taylor and Francis, London.

Stecher G., Tamura K. & Kumar S. 2018. Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Molecular Biology and Evolution 37 (4): 1237–1239. https://doi.org/10.1093/molbev/msz312

Taylor E.H. 1922. The Snakes of the Philippine Islands. Dalcassian Publishing Company, USA.

Theobald W. 1876. Descriptive Catalogue of the Reptiles of British India. The Indian Museum, Calcutta. https://doi.org/10.5962/bhl.title.5483

Trifinopoulos J., Nguyen L.T., von Haeseler A. & Minh B.Q. 2016. Nucleic Acids Research 44 (W1): W232–W235. https://doi.org/10.1093/nar/gkw256

Uetz P., Hallermann J. & Hosek J. 2021. EMBL Reptile Database. Genus: Ahaetulla. Available from https://reptiledatabase.reptarium.cz/ [accessed 1 Jul. 2022].

Wall F. 1906. A popular treatise on the common Indian snakes. Journal of the Bombay Natural History Society 17 (1): 1–9. https://www.biodiversitylibrary.org/part/24098

Wall F. 1909a. Notes on snakes from the neighbourhood of Darjeeling. Journal of the Bombay Natural History Society 19 (2): 337–357. https://www.biodiversitylibrary.org/part/33733

Wall F. 1909b. Notes on snakes collected in the Jalpaiguri district. Journal of the Bombay Natural History Society 19 (3): 897–700. Available from https://www.biodiversitylibrary.org/part/220519 [accessed 1 Jul. 2022].

Wall F. 1910. Notes on snakes collected in upper Assam. Part II. Journal of the Bombay Natural History Society 19: 825–845. https://www.biodiversitylibrary.org/item/28012#page/939/mode/1up

Wallach V., Williams K.L. & Boundy J. 2014. Snakes of the World: A Catalogue of Living and Extinct Species. Taylor and Francis, CRC Press.

Whitaker R. & Captain A. 2004. Snakes of India – The Field Guide. Draco Books, India.

Zhang J., Kapli P., Pavlidis P. & Stamatakis A. 2013. A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29 (22): 2869–2876. https://doi.org/10.1093/bioinformatics/btt499

Published
2022-10-07
How to Cite
Srikanthan, A. N., Adhikari, O. D., Kumar Mallik, A., Campbell, P. D., Bhushan Bhatt, B., Shanker, K., & Rajagopalan Ganesh, S. (2022). Taxonomic revaluation of the Ahaetulla prasina (H. Boie in F. Boie, 1827) complex from Northeast India: resurrection and redescription of Ahaetulla flavescens (Wall, 1910) (Reptilia: Serpentes: Colubridae). European Journal of Taxonomy, 839, 120–148. https://doi.org/10.5852/ejt.2022.839.1937
Section
Research article