Distribution pattern of the Microhyla heymonsi group (Anura, Microhylidae) with descriptions of two new species from Vietnam
Abstract
We provide the first distribution pattern of the Microhyla heymonsi group based on available molecular and morphological data collected from East and Southeast Asia. Our analyses show a high level of genetic diversity in the M. heymonsi group with nine distinct lineages from China, Myanmar, Vietnam, Laos, Malaysia, Thailand, Cambodia, as well as Singapore, and Indonesia. The study also reveals the discovery of two new species in Vietnam, Microhyla hmongorum sp. nov. from Lai Chau Province and Microhyla xodangorum sp. nov. from Kon Tum Province. When comparing the 12S–16S rRNA gene, the genetic divergence between Microhyla xodangorum and other congeners of the Microhyla heymonsi group ranges from 7.5-- to 8.9% (M. cf. heymonsi) and approximately 8.4% between the new species and M. heymonsi s. str. from Taiwan, China. The genetic divergence between Microhyla hmongorum and its congeners ranges from 4.5–5.6% (M. cf. heymonsi) to 8.7% (Microhyla xodangorum). These new findings bring the total number of known species in the genus Microhyla to 48 and the recorded species of Microhyla from Vietnam to 14.
References
AmphibiaWeb. 2022. University of California, Berkeley, CA, USA. Available from https://amphibiaweb.org [accessed 15 Oct. 2022].
Andersson L.G. 1942. A small collection of frogs from Annam collected in the years 1938–39 by Bertil Bjorkegren. Arkiv för Zoologi 34A (6): 1–11.
Atmaja V.Y., Hamidy A., Arisuryanti T., Matsui M. & Smith E.N. 2018. A new species of Microhyla (Anura: Microhylidae) from Sumatra, Indonesia. Treubia 45: 25–46.
Bain R.H. & Nguyen Q.T. 2004. Three new species of narrow-mouth frogs (genus: Microhyla) from Indochina, with comments on Microhyla annamensis and Microhyla palmipes. Copeia 2004 (3): 507–524. https://doi.org/10.1643/ch-04-020r2
Boulenger G.A. 1897. Descriptions of new Malay frogs. Annals and Magazine of Natural History, Series 6 19 (109): 106–108. https://doi.org/10.1080/00222939708680508
Boulenger G.A. 1900. Descriptions of new batrachians and reptiles from the Larut Hills, Perak. Annals and Magazine of Natural History, Series 7 6 (32): 186–193. https://doi.org/10.1080/00222930008678356
Boulenger G.A. 1920. IX. Descriptions of three new frogs in the collection of the British Museum. Journal of Natural History 6: 106–108. https://doi.org/10.1080/00222932008632416
Bourret R. 1942. Les Batraciens de l’Indochine. Institut océanographique de l’Indochine, Ha Noi.
Das I. & Haas A. 2010. New species of Microhyla from Sarawak: Old World’s smallest frogs crawl out of miniature pitcher plants on Borneo (Amphibia: Anura: Microhylidae). Zootaxa 2571 (1): 37–52. https://doi.org/10.11646/zootaxa.2571.1.2
Das I., Yaakob N.S. & Sukumaran J. 2007. A new species of Microhyla (Anura: Microhylidae) from the Malay Peninsula. Hamadryad 31 (2): 304–314.
Dutta S.K. & Manamendra-Arachchi K. 1996. The Amphibian Fauna of Sri Lanka. Wildlife Heritage Trust of Sri Lanka, Colombo.
Dutta S.K. & Ray P. 2000. Microhyla sholigari, a new species of microhylid frog (Anura: Microhylidae) from Karnataka, India. Hamadryad 25: 38–44.
Fei L., Ye C.Y. & Jiang J.P. 2012. Colored Atlas of Chinese Amphibians and Their Distributions. Sichuan Publishing Group, Sichuan Publishing House of Science and Technology, Sichuan.
Fei L., Hu S.-Q., Ye C.-Y. & Huang Y.-Z. 2009. Fauna Sinica. Amphibia. Vol. 2, Anura. Science Press, Beijing.
Fernando P. & Siriwardhane M. 1996. Microhyla karunaratnei (Anura: Microhylidae), a new species of frog endemic to Sri Lanka. Journal of South Asian Natural History 2: 135–142.
Frost D.R. 2021. Amphibian Species of the World: An Online Reference. Version 6.1. American Museum of Natural History, New York. Available from http://research.amnh.org/herpetology/amphibia/index.html [accessed 17 Jun. 2021].
Frost D.R., Grant T., Faivovich J., Bain R., Haas A., Haddad C.F.B., de Sa R.O., Channing A., Wilkinson M., Donnellan S.C., Raxworthy C., Campbell J.A., Blotto B.L., Moler P., Drewes R.C., Nussbaum R.A., Lynch J.D., Green D.M. & Wheeler W.C. 2006. The amphibian tree of life. Bulletin of the American Museum of Natural History 297: 1–291.
Garg S., Suyesh R., Das A., Jiang J.P., Wijayathilaka N., Amarasinghe A.A.T., Alhadi F., Vineeth K.K., Aravind N.A., Senevirathne G., Meegaskumbura M. & Biju S.D. 2019. Systematic revision of Microhyla (Microhylidae) frogs of South Asia: a molecular, morphological, and acoustic assessment. Vertebrate Zoology 69: 1–71.
Gorin V.A., Solovyeva E.N., Hasan M., Okamiya H., Karunarathna D.M.S.S., Pawangkhanant P., de Silva A., Juthong W., Milto K.D., Nguyen L.T., Suwannapoom C., Haas A., Bickford D.P., Das I. & Poyarkov N.A. 2020. A little frog leaps a long way: compounded colonizations of the Indian Subcontinent discovered in the tiny Oriental frog genus Microhyla (Amphibia: Microhylidae). PeerJ 8: e9411. https://doi.org/10.7717/peerj.9411
Gorin V.A., Scherz M.D., Korost D.V. & Poyarkov N.A. 2021. Consequences of parallel miniaturisation in Microhylinae (Anura, Microhylidae), with the description of a new genus of diminutive South East Asian frogs. Zoosystematics and Evolution 97 (1): 21–54. https://doi.org/10.3897/zse.97.57968
Goutte S., Dubois A., Howard S.D., Marquez R., Rowley J.J., Dehling J.M., Grandcolas P., Xiong R. & Legendre F. 2016. Environmental constraints and call evolution in torrent dwelling frogs. Evolution 70 (4): 811–826.
Grosjean S., Ohler A., Chuaynkern Y., Cruaud C. & Hassanin A. 2015. Improving biodiversity assessment of anuran amphibians using DNA barcoding of tadpoles. Case studies from Southeast Asia. Comptes Rendus Biologies 338 (5): 351–361.
Hall T.A. 1999. BioEdit: a User-friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT: 95–98. Nucleic Acids Symposium Series, Information Retrieval Ltd, London.
Hammer O., Harper D.A.T. & Ryan P.D. 2001. PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electonica 4 (1): 1–9.
Hasan M., Islam M.M., Khan M.M.R., Alam M.S., Kurabayashi A., Igawa T., Kuramoto M. & Sumida M. 2012. Cryptic anuran biodiversity in Bangladesh revealed by mitochondrial 16S rRNA gene sequences. Zoological Science 29 (3): 162–172. https://doi.org/10.2108/zsj.29.162
Hasan M., Islam M.M., Kuramoto M., Kurabayashi A. & Sumida M. 2014. Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh. Zootaxa 3755 (5): 401–408. https://doi.org/10.11646/zootaxa.3755.5.1
Hasan M., Razzaque M.A., Sarker A.K., Kuramoto M. & Sumida M. 2015. Genetic variation, advertisement call, and morphometry of Microhyla nilphamariensis from Bangladesh. Philippine Journal of Systematic Biology 9: 63–80.
Hillis D.M. & Bull J.J. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42: 182–192. https://doi.org/10.1093/sysbio/42.2.182
Hoang V.C., Nguyen T.T., Luong M.A., Nguyen Q.T., Orlov N., Chen Y.H., Wang B. & Jiang J.P. 2020. A new species of Microhyla (Amphibia: Anura: Microhylidae) from Langbian Plateau, Central Vietnam. Asian Herpetological Research 11(3): 161–182. https://doi.org/10.16373/j.cnki.ahr.190060
Hoang V.C., Nguyen T.T., Ninh T.H., Luong M.A., Pham T.C., Nguyen Q.T., Orlov N., Chen Y.H., Wang B., Ziegler T. & Jiang J.P. 2021. Two new cryptic species of Microhyla Tschudi, 1838 (Amphibia, Anura, Microhylidae) related to the M. heymonsi group from central Vietnam. ZooKeys 1036: 47–74. https://doi.org/10.3897/zookeys.1036.56919
Howlader M.S.A., Nair A., Gopalan S.V. & Merilä J. 2015. A new species of Microhyla (Anura: Microhylidae) from Nilphamari, Bangladesh. PLoS One 10 (3): e0119825. https://doi.org/10.1371/journal.pone.0119825
Huelsenbeck J.P. & Hillis D.M. 1993. Success of phylogenetic methods in the four-taxon case. Systematic Biology 42 (3): 247–264. https://doi.org/10.2307/2992463
Inger R.F. 1966. The systematics and zoogeography of the Amphibia of Borneo. Fieldiana, Zoology 52: 1–402.
Inger R.F. 1989. Four new species of frogs from Borneo. Malayan Nature Journal, Kuala Lumpur 42: 229–243.
Inger R.F. & Frogner K.J. 1979. New species of narrow-mouth frogs (genus Microhyla) from Borneo. The Sarawak Museum Journal 27: 311–322.
Khatiwada J.R., Shu G.C., Wang S.H., Thapa A., Wang B. & Jiang J.P. 2017. A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal. Zootaxa 4254 (2): 221–239. https://doi.org/10.11646/zootaxa.4254.2.4
Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120. https://doi.org/10.1007/BF01731581
Krzikowski M., Nguyen T.Q., Pham C.T., Rödder D., Rauhaus A., Le M.D. & Ziegler T. 2022. Assessment of the threat status of the amphibians in Vietnam - implementation of the One Plan Approach. Nature Conservation 49: 77–116. https://doi.org/10.3897/natureconservation.49.82145
Kumar S., Stecher G., Li M., Knyaz C. & Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
Kuraishi N., Matsui M., Hamidy A., Belabut D.M., Ahmad N., Panha S., Sudin A., Yong H.S., Jiang J.P., Ota H., Thong H.T., & Nishikawa K. 2012. Phylogenetic and taxonomic relationships of the Polypedates leucomystax complex (Amphibia). Zoologica Scripta 42: 54–70.
Kuramoto M. & Joshy S.H. 2006. Morphological and acoustic comparisons of Microhyla ornata, M. fissipes, and M. okinavensis (Anura: Microhylidae). Current Herpetology 25: 15–27.
Leaché A.D. & Reeder T.W. 2002. Molecular systematics of the eastern fence lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology 51: 44–68. https://doi.org/10.1080/106351502753475871
Li S., Zhang M., Xu N., Lu J., Jiang J.P., Liu J., Wei G. & Wang B. 2019. A new species of the genus Microhyla (Amphibia: Anura: Microhylidae) from Guizhou Province, China. Zootaxa 4624 (4): 551–575. https://doi.org/10.11646/zootaxa.4624.4.7
Matsui M. 2011. Taxonomic revision of one of the Old World’s smallest frogs, with description of a new Bornean Microhyla (Amphibia, Microhylidae). Zootaxa 2814 (1): 33–49. https://doi.org/10.11646/zootaxa.2814.1.3
Matsui M., Ito H., Shimada T., Ota H., Saidapur S.K., Khonsue W., Tanaka-Ueno T. & Wu G.F. 2005. Taxonomic relationships within the Pan-Oriental narrow-mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae). Zoological Science 22 (4): 489–495. https://doi.org/10.2108/zsj.22.489
Matsui M., Hamidy A., Belabut D.M., Ahmad N., Panha S., Sudin A., Khonsue W., Oh H.S., Yong H.S. & Jiang J.P. 2011. Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenetics and Evolution 61 (1): 167–176. https://doi.org/10.1016/j.ympev.2011.05.015
Matsui M., Hamidy A. & Eto K. 2013. Description of a new species of Microhyla from Bali, Indonesia (Amphibia, Anura). Zootaxa 3670 (4): 579–590. https://doi.org/10.11646/zootaxa.3670.4.9
Nguyen L.T., Poyarkov N.A., Nguyen T.T., Nguyen T.A., Tran V.H., Gorin V.A., Murphy R.W. &
Nguyen S.N. 2019. A new species of the genus Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from Tay Nguyen Plateau, Central Vietnam. Zootaxa 4543 (4): 549–580. https://doi.org/10.11646/zootaxa.4543.4.4
Nguyen T.T., Matsui M., Eto K. & Orlov N.L. 2014. A preliminary study of phylogenetic relationships and taxonomic problems of Vietnamese Rhacophorus (Anura: Rhacophoridae). Russian Journal Herpetoloy 21 (4): 274–280.
Nguyen T.T., Pham T.C., Nguyen T.Q., Ninh H.T. & Ziegler T. 2017. A new species of Rhacophorus (Amphibia: Anura: Rhacophoridae) from Vietnam. Asian Herpetological Research 8: 221–234.
Parker H.W. 1928. LXIII. The brevicipitid frogs of the genus Microhyla. Journal of Natural History 2 (11): 473–499. https://doi.org/10.1080/00222932808672911
Parker H.W. 1934. Monograph of the Frogs of the Family Microhylidae. Trustees of the British Museum, London.
Parker H.W. & Osman H.W.C. 1948. Frogs of the genus Microhyla from Ceylon. Journal of Natural History 1 (10): 759–764. https://doi.org/10.1080/00222934808653944
Pillai R. 1977. On two frogs of the family Microhylidae from Andamans including a new species. Proceedings of the Indian Academy of Sciences, Section-B 86: 135–138. https://doi.org/10.1007/BF03050916
Poyarkov N.A., Vassilieva A.B., Orlov N.L., Galoyan E.A., Tran D., Le D.T.T., Kretova V.D. & Geissler P. 2014. Taxonomy and distribution of narrow-mouth frogs of the genus Microhyla Tschudi, 1838 (Anura: Microhylidae) from Vietnam with descriptions of five new species. Russian Journal of Herpetology 21 (2): 89–148.
Poyarkov N.A., Gorin V.A., Zaw T., Kretova V.D., Gogoleva S.I., Pawangkhanant P. & Che J. 2019. On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species. Zoological Research 40 (4): 244–276. https://doi.org/10.24272/j.issn.2095-8137.2019.044
Poyarkov N.A. jr., Nguyen T.V., Trofimets A.V. & Gorin V.A. 2020. A new cryptic species of the genus Microhyla (Amphibia: Microhylidae) from Langbian Plateau, Vietnam. Taprobanica. The Journal of Asian Biodiversity 9: 136–163.
Rambaut A. & Drummond A. 2009. TRACER, version 1.5. Available from http://beast.bio.ed.ac.uk/Tracer [accessed 30 Sep. 2022].
Ronquist F., Teslenko M., van der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A. & Huelsenbeck J.P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Sa de R.O., Streicher J.W., Sekonyela R., Forlani M.C., Loader S.P., Greenbaum E., Richards S. & Haddad C.F. 2012. Molecular phylogeny of microhylid frogs (Anura: Microhylidae) with emphasis on relationships among New World genera. BMC Ecology and Evolution 12 (1): e241. https://doi.org/10.1186/1471-2148-12-241
Savage J.M. 1975. Systematics and distribution of the Mexican and Central American stream frogs related to Eleutherodactylus rugulosus. Copeia 1975: 254–306. https://doi.org/10.2307/1442883
Schenkel E. 1901. Achter Nachtrag zum Katalog der herpetologischen Sammlung des Basler Museums. Verhandlungen der Naturforschenden Gesellschaft in Basel 13: 142–199.
Seshadri K.S., Singal R., Priti H., Ravikanth G., Vidisha M.K., Saurabh S., Pratik M. & Gururaja K.V. 2016. Microhyla laterite sp. nov., a new species of Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from a laterite rock formation in South West India. PLoS ONE 11 (3): e0149727. https://doi.org/10.1371/journal.pone.0149727
Simmons J.E. 2002. Herpetological collecting and collections management. Revised edition. Society for the Study of Amphibians and Reptiles. Herpetological Circular 31: 1–153.
Simon C., Frati F., Beckenbach A., Crespi B., Liu H. & Flook P. 1994. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87 (6): 651–701. https://doi.org/10.1093/aesa/87.6.651
Smith M. 1923. Notes on reptiles and batrachians from Siam and Indo-China (No. 2). Journal of the Natural History Society of Siam 6 (1): 47–53.
Tanabe A.S. 2011. Kakusan 4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources 11: 914–921. https://doi.org/10.1111/j.1755-0998.2011.03021.x
Tavaré S. 1986. Some probabilistic and statistical problems in the analysis of DNA sequences. Lectures on Mathematics in the Life Sciences 17: 57–86.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25 (24): 4876–4882. https://doi.org/10.1093/nar/25.24.4876
Tominaga A., Matsui M., Shimoji N., Khonsue W., Wu C.S., Toda M., Eto K., Nishikawa K. & Ota H. 2019. Relict distribution of Microhyla (Amphibia: Microhylidae) in the Ryukyu Archipelago: high diversity in East Asia maintained by insularization. Zoologica Scripta 48: 440–453. https://doi.org/10.1111/zsc.12361
Tschudi J.J. 1838. Classification der Batrachier mit Berücksichtigung der fossilen Thiere dieser Abtheilung der Reptilien. Petitpierre, Neuchâtel. https://doi.org/10.5962/bhl.title.4883
Vogt T. 1913. Über die Reptilien- und Amphibienfanna der Insel Hainan. Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin 1913: 219–221.
Vineeth K.K., Radhakrishna U.K., Godwin R.D., Anwesha S., Rajashekhar K.P. & Aravind N.A. 2018. A new species of Microhyla Tschudi, 1838 (Anura: Microhylidae) from West Coast of India: an integrative taxonomic approach. Zootaxa 4420: 151–179. https://doi.org/10.11646/zootaxa.4420.2.1
Whitish F. & Vences M. 1997. Anuran eye colouration: definitions, variation, taxonomic implications and possible functions. In: Bohme W., Bischoff W. & Ziegler T. (eds) Herpetologia Bonnensis: 125–138. Societas Europaea Herpetologica, Bonn.
Wijayathilaka N., Garg S., Senevirathne G. & Karunarathna N. 2016. A new species of Microhyla (Anura: Microhylidae) from Sri Lanka: an integrative taxonomic approach. Zootaxa 4066 (3): 331–342. https://doi.org/10.11646/zootaxa.4066.3.9
Yuan Z.Y., Suwannapoom C., Yan F., Poyarkov N.A., Nguyen S.N., Chen H.M., Chomdej S., Murphy R.W. & Che J. 2016. Red River barrier and Pleistocene climatic fluctuations shaped the genetic structure of Microhyla fissipes complex (Anura: Microhylidae) in southern China and Indochina. Current Zoology 62 (6): 531–543. https://doi.org/10.1093/cz/zow042
Zhang M.H., Fei L., Ye C.Y., Wang Y.F., Wang B. & Jiang J.P. 2018. A new species of genus Microhyla (Amphibia: Anura: Microhylidae) from Zhejiang Province, China. Asian Herpetological Research 9: 135–148.
Zhang P., Zhou H., Chen Y.Q., Liu Y.F. & Qu L.H. 2005. Mitogenomic perspectives on the origin and phylogeny of living amphibians. Systematic Biology 54 (3): 391–400.
Zhang P., Papenfuss T.J., Wake M.H., Qu L. & Wake D.B. 2008. Phylogeny and biogeography of the family Salamandridae (Amphibia: Caudata) inferred from complete mitochondrial genomes. Molecular Phylogenetics and Evolution 49 (2): 586–597. https://doi.org/10.1016/j.ympev.2008.08.020
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