Two new Asian species of Russula sect. Ingratae with unique basidiospore features for subg. Heterophyllidiae

  • Aniket Ghosh Cryptogamic Unit, Central National Herbarium, Botanical Survey of India, 3rd MSO Building DF block, Sector 1, Salt Lake City, Kolkata – 700064, India
  • Bart Buyck Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum national d’histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles CP 39, 57 rue Cuvier, 75005 Paris, France
  • Kanad Das Central National Herbarium, Botanical Survey of India, P.O. – Botanic Garden, Howrah – 711103, India
  • Ishika Bera Central National Herbarium, Botanical Survey of India, P.O. – Botanic Garden, Howrah – 711103, India
  • Dyutiparna Chakraborty Eastern Regional Center, Botanical Survey of India, Shillong – 793003, India
Keywords: macrofungi, nrITS, phylogeny, Russulales, taxonomy


Two novel species of Russula (Russulaceae, Russulales), namely Russula indosenecis A.Ghosh, D.Chakr., K.Das & Buyck sp. nov. and R. pseudosenecis A.Ghosh, D.Chakr., K.Das & Buyck sp. nov. belonging to sect. Ingratae subg. Heterophyllidiae are proposed herein based on their morphological features and nrITS-based phylogenetic inferences. Both species belong to the Asian ʻR. punctipes-senecisʼ complex of sect. Ingratae. The acrid R. indosenecis was collected from subalpine forests associated with Abies densa, whereas the mild R. pseudosenecis associates with tropical forests dominated by Shorea robusta. Both species are distinct from the other species of this species complex in nrITS sequence data and from all other known species in subg. Heterophyllidiae in the strong amyloidity of their suprahilar spot.


Adamčík S., Looney B., Caboň M., Jančovičová S., Adamčíková K., Avis P.G., Barajas M., Bhatt R.P., Corrales A., Das K., Hampe F., Ghosh A., Gates G., Kälviäinen V., Khalid A.K., Kiran M., De Lange R., Lee H., Lim Y.W., Luz A.K., Manz C., Ovrebo C., Park J.Y., Saba M., Taipale T., Verbeken A., Wisitrassameewong K. & Buyck B. 2019. The quest for a globally comprehensible Russula language. Fungal Diversity 99 (1): 369–449.

Bi Z.S. & Li T.H. 1986. A preliminary note on Russula species from Guangdong, with a new species and a new variety. Guihaia 6 (3): 193–199.

Buyck B. 1989. Etudes microscopiques de Russules tropicales: Mimeticinae subsect. nov. Mycotaxon 35: 55–63.

Buyck B. 1994. Flore illustrée des Champignons d’Afrique centrale 16. Russula II (Russulaceae) 16: 411–542. Jardin Botanique National de Belgique.

Buyck B., Zoller S. & Hofstetter V. 2018. Walking the thin line… ten years later: the dilemma of above-versus below-ground features to support phylogenies in the Russulaceae (Basidiomycota). Fungal Diversity 89 (1): 267–292.

Chen B., Song J., Zhang J-H. & Liang J.-F. 2021. Morphology and molecular phylogeny reveal two new species in Russula sect. Ingratae from China. Phytotaxa 525 (2): 109–123.

Chen Z., Zhang P. & Zhang Z. 2014. Investigation and analysis of 102 mushroom poisoning cases in Southern China from 1994 to 2012. Fungal Diversity 64: 123–131.

Chua L.S.L., Lee S.S., Alias S.A., Jones E.G.B., Zainuddin N. & Chan H.T. 2012. Checklist of Fungi of Malaysia, Issue/No. 132. Forest Research Institute Malaysia (FRIM), Malaysia.

Crous P.W., Wingfield M.J., Burgess T.I., Hardy G.E.S.J., Barber P.A., Alvarado P., Barnes C.W., Buchanan P.K., Heykoop M., Moreno G., Thangavel R., Van der Spuy S., Barili A., Barrett S., Cacciola S.O., Cano-Lira J.F., Crane C., Decock C., Gibertoni T.B., Guarro J., Guevara-Suarez M., Hubka V., Kolařík M., Lira C.R.S., Ordoñez M.E., Padamsee M., Ryvarden L., Soares A.M., Stchigel A.M., Sutton D.A., Vizzini A., Weir B.S., Acharya K., Aloi F., Baseia I.G., Blanchette R.A., Bordallo J.J., Bratek Z., Butler T., Cano-Canals J., Carlavilla J.R., Chander J., Cheewangkoon R., Cruz R.H.S.F., Da Silva M., Dutta A.K., Ercole E., Escobio V., Esteve-Raventós F., Flores J.A., Gené J., Góis J.S., Haines L., Held B.W., Horta Jung M., Hosaka K., Jung T., Jurjević Ž., Kautman V., Kautmanova I., Kiyashko A.A., Kozanek M., Kubátová A., Lafourcade M., La Spada F., Latha K.P.D., Madrid H., Malysheva E.F., Manimohan P., Manjón J.L., Martín M.P., Mata M., Merényi Z., Morte A., Nagy I., Normand A.-C., Paloi S., Pattison N., Pawłowska J., Pereira O.L., Petterson M.E., Picillo B. & Raj K.N.A. 2017. Fungal Planet description sheets: 558–624. Persoonia 38: 240–384.

Das K., Sharma J.R. & Atri N.S. 2006. Russula in Himalaya 3: A new species of subgenus Ingratula. Mycotaxon 95: 271–275.

Das K., Van de Putte K. & Buyck B. 2010. New or interesting Russula from Sikkim Himalaya (India). Cryptogamie Mycologie 31 (4): 373‒387.

Das K., Ghosh A., Chakraborty D., Li J., Qiu L., Baghela A., Halama M., Hembrom M.E., Mehmood T., Parihar A., Pencakowski B., Bielecka M., Reczynska K., Sasiela D., Singh U., Song Y., Swierkosz K., Szczesniak K., Uniyal P., Zhang J. & Buyck B. 2017. Fungal Biodiversity Profiles 31–40. Cryptogamie Mycologie 38 (3): 1–56.

Drummond A.J., Ashton B., Buxton S., Cheung M., Cooper A., Heled J., Kearse M., Moir R., Stones-Havas S., Sturrock S., Thierer T. & Wilson A. 2010. Geneious ver. 5.1. Available from [accessed 31 Oct. 2022].

Edler D., Klein J., Antonelli A. & Silvestro D. 2021. raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods in Ecology and Evolution 12: 373–377.

Gardes M & Bruns T.D. 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118.

Ghosh A., Das K., Bhatt R.P. & Hembrom M.E. 2020. Two new species of the Genus Russula from western Himalaya with morphological details and phylogenetic estimations. Nova Hedwigia 111 (1–2): 115–130.

Ghosh A., Das K. & Buyck B. 2021. Two new species in the Russula (Russulaceae, Basidiomycota) crown clade from Indian Himalaya. European Journal of Taxonomy 782: 157–172.

Hackel J., Henkel T.W., Moreau P.-A., De Crop E., Verbeken A., Sà M., Buyck B., Neves M.-A., Vasco-Palacios A., Wartchow F., Schimann H., Carriconde F., Garnica S., Courtecuisse R., Gardes M., Manzi S., Louisanna E. & Roy M. 2022. Biogeographic history of a large clade of ectomycorrhizal fungi, the Russulaceae, in the Neotropics and adjacent regions. New Phytologist 236 (2): 698–713.

Han Y.-X., Liang Z.-Q., Jiang S. & Zeng N.-K. 2022. Russula hainanensis (Russulaceae, Russulales), a new species from tropical China. Phytotaxa 552 (1): 35–50.

Imai S. 1938. Studies on the Agaricaceae of Hokkaido. II. Journal of the Faculty of Agriculture of the Hokkaido Imperial University 43: 179–378.

Katoh K., Rozewicki R. & Yamada K.D. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20 (4): 1160–1166.

Khatua S., Dutta A.K. & Acharya K. 2015. Prospecting Russula senecis: A delicacy among the tribes of West Bengal. PeerJ 3: e810.

Kõljalg U., Nilsson R.H., Abarenkov K., Tedersoo L., Taylor A.F.S., Bahram M., Bates S.T., Bruns T.D., Bengtsson-Palme J., Callaghan T.M., Douglas B., Drenkhan T., Eberhardt U., Dueñas M., Grebenc T., Griffith G.W., Hartmann M., Kirk P.M., Kohout P., Larsson E., Lindahl B.D., Lücking R., Martín M.P., Matheny P.B., Nguyen N.H., Niskanen T., Oja J., Peay K.G., Peintner U., Peterson M., Põldmaa K., Saag L., Saar I., Schüßler A., Scott J.A., Senés C., Smith M.E., Suija A., Taylor D.L., Telleria M.T., Weiss M. & Larsson K.-H. 2013. Towards a unified paradigm for sequence-based identification of Fungi. Molecular ecology 22: 5271–5277.

Kornerup A. & Wanscher J.H. 1978. Methuen Handbook of Colour, 3rd Edition. Methuen, London.

Lanfear R., Frandsen P.B., Wright A.M., Senfeld T. & Calcott B. 2017. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34 (3): 772–773.

Li G.-J., Li S.-M., Buyck B., Zhao S.-Y., Xie X.-J., Shi L.-Y., Deng C.-Y., Meng Q.-F., Sun Q.-B., Jun-Qing Yan J.-Q., Wang J. & Li M. 2021. Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China. MycoKeys 84: 103–139.

Natarajan K., Senthilrasu G., Kumaresan V. & Riviera T. 2005. Diversity in Ectomycorrhizal fungi of a dipterocarp forest in Western Ghats. Current science 88 (12): 1893–1895.

Romagnesi H. 1967. Les Russules d’Europe et d’Afrique du Nord. Bordas, Paris.

Rambaut A., Suchard M.A., Xie D., & Drummond A.J. 2014. Tracer version 1.6. Available from [accessed 31 Oct. 2022].

Ronquist F., Teslenko M., van der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A. & Huelsenbeck J.P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542.

Sarnari M. 1998. Monografia Illustrata del Genre Russula in Europa, Prima Parte. Associazione Micologica Bresadola, Trento.

Singer R. 1935. Supplemente zu meiner Monographie der Gattung Russula. Annales Mycologici 33: 297–352.

Singer R. 1986. The Agaricales in Modern Taxonomy. 4th Edition. Koeltz Scientific Books, Koenigstein.

Song J., Chen B., Liang J.-F., Li H.-J., Wang S.-K. & Lu J.-K. 2020. Morphology and phylogeny reveal Russula subpunctipes sp. nov., from southern China. Phytotaxa 459 (1): 016–024.

Song Y., Buyck B., Li J.W., Yuan F., Zhang Z.W. & Qiu L.H. 2018. Two novel and a forgotten Russula species in sect. Ingratae (Russulales) from Dinghushan Biosphere Reserve in southern China. Cryptogamie, Mycologie 39: 341–357.

Vellinga E.C. 1988. Glossary. In: Bas C., Kuyper T.H.W., Noordeloos M.E. & Vellinga E.C. (eds) Flora Agaricina Neerlandica, Supplemente zu meiner Monographie der Gattung Russula Vol. 1. A.A. Balkema, Rotterdam. Netherlands.

White T.J., Bruns T., Lee S.S. & Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J. & White T.J. (eds) PCR Protocols: a Guide to Methods and Applications: 315–322. Academic Press, New York.

Yuan H.-S., Lu Xu., Dai Yu.-C., Hyde K.D., Kan Yu.-H., et al. 2020. Fungal diversity notes 1277–1386: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 104: 1–266.

How to Cite
Ghosh, A., Buyck, B., Das, K., Bera, I., & Chakraborty, D. (2022). Two new Asian species of Russula sect. Ingratae with unique basidiospore features for subg. Heterophyllidiae. European Journal of Taxonomy, 847(1), 104-120.
Research article