New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

  • Benoît Véron Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France
  • Etienne Rougier Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France
  • Anthony Taylor Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France
  • Didier Goux Normandie Université, UNICAEN, CMAbio3 (Centre de Microscopie Appliquée à la Biologie), SF EMerode, 14000 Caen, France
Keywords: Haptophytes, phytoplankton, habitats, new species, pyrenoid


The justification of the 4 genera that currently compose the class Pavlovophyceae is based on a low number of species and a relative paucity of available, traceable and referenced cultures. Previous integrative phylogeny work revealed strains that can refine and strengthen our knowledge of the genera in the class. The application of multiple light and electron microscopy techniques allowed us to prioritize the cytomorphological characters (pyrenoid, thylakoid, stigma, knob-scales, life stage / life cycle) used for the taxonomy of these algae and to describe two new species: Exanthemachrysis fresneliae Véron sp. nov. and Rebecca billardiae Véron sp. nov. Consequently, revisions of the two genera Exanthemachrysis Lepailleur emend. Véron and Rebecca Green emend. Véron were made. In addition, the genus Pavlova Butcher emend Véron is revised in the light of these characters. Particular emphasis is placed on the life stages and habitat of the species.


Attoui H., Jaafar F.M., Belhouchet M., de Micco P., de Lamballerie X. & Brussaard C.P.D. 2006. Micromonas pusilla reovirus: a new member of the family Reoviridae assigned to a novel proposed genus (Mimoreovirus). Journal of General Virology 87 (5):1375–1383.

Bazin P., Jouenne F., Friedl T., Deton-Cabanillas A.-F., Le Roy B. & Véron B. 2014. Phytoplankton diversity and community composition along the estuarine gradient of a temperate macrotidal ecosystem: Combined morphological and molecular approaches. PLoS ONE 9 (4): e94110.

Bendif E.L., Probert I., Hervé A., Billard C., Goux D., Lelong C., Cadoret J.-P. & Véron B. 2011. Integrative taxonomy of the Pavlovophyceae (Haptophyta): A reassessment. Protist 162: 738–761.

Bourrelly P. 1957. Recherches sur les Chrysophycées. Morphologie, phylogénie, systématique. Revue Algologique – Mémoire Hors-Série 1: 1-412

Butcher R.W. 1952. Contributions to our knowledge of the smaller marine algae. Journal of the Marine Biological Association of the United Kingdom 31: 175–191.

Carter N. 1937. New and interesting algae from brackish water. Archiv für Protistenkunde 90: 1–68.

Cosson J. 1987. Croissance des sporophytes résultats d’hybridations interspécifiques et intergénérique chez les laminaires. Crytogamie, Algologie 8: 61–72.

Edvardsen B., Eikrem W., Green J.C., Andersen R.A., Moon-van-der-Staay S.Y. & Medlin L.K. 2000. Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data. Phycologia 39: 19–35.

Edvardsen B., Egge E.S. & Vaulot D. 2016. Diversity and distribution of haptophytes revealed by environmental sequencing and metabarcoding – a review. Perspectives in Phycology 3: 77–91.

Egge E.S., Johannessen T.V., Andersen T., Eikrem W., Bittner L., Larsen A., Sandaa R.A. & Edvardsen B. 2015. Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing. Molecular ecology 24: 3026–3042.

Eikrem W., Medlin L., Hendriks J., Rokitta S., Rost B., Probert I., Throndsen J. & Edvardsen B. 2017. Haptophyta. In: Archibald J.M., Simpson A.G.B, Slamovits C.H., Margulis L., Melkonian M., Chapman D.J. & Corliss J.O. (eds) Handbook of the Protists: 1–61. Springer, Cham.

Gayral P. & Fresnel J. 1979. Exanthemachrysis gayraliae Lepailleur (Prymnesiophyceae, Pavlovales): Ultrastructure et discussion taxinomique. Protistologica XV: 271–282.

Geitler L. 1969. Beobachtungne über Chrysocapsella granifera. Österreichische botanische Zeitschrift 117: 365–371.

Geitler L. 1971. Weitere Untersuchungen über ein Massenvorkommen von Chrysocapsella granifera im Linzer Unteresee mit Zonenbildung. Archiv für Hydrobiologie 68: 516–518.

Green J.C. 1967. A new species of Pavlova from Madeira. British Phycological Bulletin 3: 299–303.

Green J.C. 1973. Studies in the fine structure and taxonomy of flagellates in the genus Pavlova. II. A freshwater representative, Pavlova granifera (Mack) comb. nov. British Phycological Journal 8: 1–12.

Green J.C. 1976. Notes on the flagellar apparatus and taxonomy of Pavlova mesolychnon Van der Veer, and on the status of Pavlova Butcher and related genera within the Haptophyceae. Journal of the Marine Biological Association of the United Kingdom 56: 595–602.

Green J.C. 1980. The fine structure of Pavlova pinguis Green and a preliminary survey of the order Pavlovales (Prymnesiophyceae). British Phycological Journal 15: 151–191.

Green J.C. & Manton I. 1970. Studies in the fine structure and taxonomy of the flagellates in the genus Pavlova I. A revision of Pavlova gyrans, the type species. Journal of the Marine Biological Association of the United Kingdom 50: 1113–1130.

Guillard R.R.L. & Ryther J.H. 1962. Studies of marine planktonic diatoms. 1. Cyclotella nana Hustedt and Detonula confaervacea Cleve. Canadian Journal of Microbiology 8: 229–239.

Jones H.L.J., Leadbeater B.S.C. & Green J.C. 1994. Mixotrophy in prymnesiophytes. In: Green J.C. & Leadbeater B.S.C. (eds) The Haptophyte Algae: 247–263. Systematics Association Special, 51. Clarendon Press, Oxford.

Jordan R.W., Cros L. & Young J.R. 2004. A revised classification scheme for living haptophytes. Micropaleontology 55 (Suppl. 1): 55–79.

Kawachi M., Nakayama T., Kayama M., Tomura M., Miyashita H., Bojo O., Rhodes L., Rym S., Pienaar R.N., Probert I., Inouye I. & Kamikawa R. 2021. Rappemonds are haptophyte phytoplankton. Current Biology 31: 1–9.

Lepailleur H. 1970. Sur un nouveau genre de Chrysophycées: Exanthemachrysis nov. gen. (E. gayralii nov. sp.). Comptes Rendus de l’Académie des sciences Paris 270 : 928-931.

Mack B. 1954. Untersuchungen an Chrysophyceen. V–VII. Österreichische botanische Zeitschrift 101: 64–73.

Nagasaki K., Tomaru Y., Katanozaka N., Shirai Y., Nishida K., Itakura S. & Yamaguchi M. 2004. Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom Rhizosolenia setigera. Applied and Environmental Microbiology 70 (2): 704–711.

Not F., Siano R., Koistra W.H.C.F., Simon N., Vaulot D. & Probert I. 2012. Diversity and ecology of eukaryotic marine phytoplankton. Advances in Botanical Research 64: 1–53. Academic Press, Cambridge.

Parke M., Manton I. & Clarke B. 1955. Studies on marine flagellates. II. Three new species of Chrysochromulina. Journal of the Marine Biological Association of the United Kingdom 34: 579–609.

Plancq J., Couto J., Ijaz U.Z., Leavitt P.R. & Toney J. 2019. Next generation sequencing to identify lacustrine haptophytes in the Canadian Prairies: significance for temperature proxy applications. Journal of Geophysical Research: Biogeosciences 124: 2144–2158.

Ponis E., Probert I., Véron B., Le Coz J.R., Mathieu M. & Robert R. 2006. Nutritional value of six Pavlovophyceae for Crassostrea gigas and Pecten maximus larvae. Aquaculture 254: 544–553.

Shalchian-Tabrizi K., Reier-Røberg K., Ree D.K., Klaveness D. & Bråte J. 2011. Marine-freshwater colonizations of Haptophytes inferred from phylogeny of environmental 18S rDNA sequences. Journal of Eukaryotic Microbiology 58: 315–318.

Simon M., Lopez-Garcia P., Moreira D. & Jardillier L. 2013. New haptophyte lineages and multiple independent colonizations of freshwater ecosystems. Environmental Microbiology Reports 5: 322–32.

Strassert J.F.H., Irisarri I., Williams T.A. & Burki F. 2021. A molecular timescale for eukaryote evolution with implications for the origin of red algal-derived plastids. Nature Communications 12: 1879.

Tai V., Lawrence J.E., Lang A.S., Chan A.M., Culley A.I. & Suttle C.A. 2003. Characterization of HaRNAV, a single-strandedRNA virus causing lysis of Heterosigma akashiwo (Raphidophyceae). Journal of Phycology 39: 343–352.

Tschermak-Woess E. 1972. Über die Haptophycee Sarcinochrysis granifera aus dem Neusiedlersee. Österreichische botanische Zeitschrift 121: 235–255.

Tschermak-Woess E. 1979. Richtigllung betreffend Sarcinochrysis granifera (Mack) Tsch.-Woess (Prymnesiophyceae). Plant Systematics and Evolution 132: 343–345.

van der Veer J. 1969. Pavlova mesolychnon (Chrysophyta) a new species from the Tamar estuary, Cornwall. Acta Botanica Neerlandica 18 (4): 496–510.

van der Veer J. 1972. Pavlova helicata (Haptophyceae), a new species from the Frisian Island Schiermonnnikoog, the Neverthlands. Nova Hedwigia 23: 131–159.

van der Veer J. 1979. Pavlova and the taxonomy of flagellates, especially the Chrysomonads. PhD Thesis, Rijks-universiteit, Groningen, The Netherlands.

Zhang X., Yang S., Sun J., Qiao Y., Wang J. & Liu H. 2018. The first record of Pavlova pinguis (Pavlovophyceae, Haptophyta) in China Seas. Acta Oceanologica Sinica 37: 28–32.

Zingone A. 1995. The role of viruses in the dynamics of phytoplankton blooms. Giornale botanico italiano 129: 415–423.

How to Cite
Véron, B., Rougier, E., Taylor, A., & Goux, D. (2023). New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova. European Journal of Taxonomy, 861(1), 21-47.
Research article