Inocybe subhimalayanensis (Agaricales, Inocybaceae), a new smooth spored Inocybe species from Pakistan

  • Annum Razzaq Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore, Pakistan
  • Arooj Naseer Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore, Pakistan
  • Abdul Nasir Khalid Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore, Pakistan
Keywords: Inocybaceae, ITS, LSU, Punjab, taxonomy


During mycological explorations, a new smooth spored species, Inocybe subhimalayanensis Razzaq, Naseer & Khalid sp. nov. was collected from moist temperate sub-Himalayan region, Pakistan. Phylogeny of ITS and LSU regions of nrDNA, and morphoanatomical data make it distinct from other known species of the genus. The taxon is characterized by: a yellowish orange to brown pileus with prominent fibrillose, prominent umbo; ellipsoid to amygdaliform smooth larger basidiospores (8.4‒)8.6‒12.2(‒12.6) × (4.9‒)5.1‒7.1(‒7.3) µm; and lack of velipellis. Molecular phylogenetic analyses further support the recognition of the new species.


Bandini D., Oertel B. & Eberhardt U. 2021. A fresh outlook on the smooth spored species of Inocybe: type studies and 18 new species. Mycological Progress 20: 1019–1114.

Bandini D., Oertel B. & Eberhardt U. 2022. More smooth-spored species of Inocybe (Agaricales, Basidiomycota): type studies and 12 new species from Europe. Persoonia 48: 91–149.

Bas C. 1969. Morphology and subdivision of Amanita and a monograph of its section Lepidella. Persoonia 5 (4): 96–97. Available from [accessed 26 April 2023].

Cripps C.L., Larsson E. & Horak E. 2010. Subgenus Mallocybe (Inocybe) in the Rocky Mountain alpine zone. North American Fungi 5 (5): 97–126.

Crous P.W., Wingfield M.J., Burgess T.I., et al. 2017. Fungal Planet description sheets: 558–624. Persoonia 38: 240–384.

Dovana F., Bizio E., Garbelotto M., et al. 2021. Inocybe cervenianensis (Agaricales,Inocybaceae), a new species in the I. flavoalbida clade from Italy. Phytotaxa 484 (2): 227–236.

Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797.

Esteve-Raventos F., Rodriguez-Campo F.J. & Alvarado P. 2017. Fungal Planet 603. Inocybe parvicystis F.J. Rodr.-Campo & Esteve-Rav., sp. nov. Persoonia 38: 338–339.

Gardes M. & Bruns T.D. 1993. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118.

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT/7. Nucleic Acids Symposium Series 41: 95–98.

Hameed M., Nawaz T., Ashraf M., Ahmad F., Ahmad K.S., Ahmad M.S.A., Raza S.H., Hussain M. & Ahmad I. 2012. Floral biodiversity and conservation status of the Himalayan foothill region, Punjab. Pakistan Journal of Botany 44: 143–149.

Jabeen S. & Khalid A.N. 2020. Pseudosperma flavorimosum sp. nov. from Pakistan. Mycotaxon 135 (1): 183–193.

Khan M.B., Naseer A., Aqdus F., Ishaq M., Fiaz M. & Khalid A.N. 2022. Inocybe quercicola sp. nov. (Agaricales, Inocybaceae), from Pakistan. Microbial Biosystems 6 (2): 22‒29.

Kokkonen K. & Vauras J. 2012. Eleven new boreal species of Inocybe with nodulose spores. Mycological Progress 11: 299–341.

Liang J.F. & Yang Z.L. 2011. A new species of Lepiota (Agaricaceae) from south western China. Mycotaxon 117: 359–363.

Matheny P.B. & Bougher N.L. 2017. Fungi of Australia, Inocybaceae. ABRS & CSIRO Publishing, Canberra & Melbourne.

Matheny P.B., Hobbs A.M., Esteve-Raventós F. 2020. Genera of Inocybaceae: New skin for the old ceremony. Mycologia 112 (1): 83–120.

Mešić A., Haelewaters D., Tkalčec Z., Liu J., Kušan I., Aime M.C. & Pošta A. 2021. Inocybe brijunica sp. nov., a new ectomycorrhizal fungus from Mediterranean Croatia revealed by morphology and multilocus phylogenetic analysis. Journal of Fungi 7: 199.

Moncalvo J.M., Lutzoni F.M., Rehner S.A., Johnson J. & Vilgalys R. 2000. Phylogenetic relationships of Agaric fungi based on nuclear large subunit ribosomal DNA sequences. Systematic Biology 49: 278–305.

Muñoz G., Pancorbo F., Turégano Y. & Esteve-Raventós F. 2022. New species and combinations of Inocybe with lilac or violet colours in Europe. Fungi Iberici 2: 7–26.

Munsell A.H. 1975. Munsell soil color charts. Baltimore, MD, USA.

Murrill W.A., Overholts L.O. & Kauffman C.H. 1932. (Agaricales): Agaricaceae (pars), Agariceae (pars). New York Botanical Garden.

Myers N., Mittermeier R.A., Mittermeier C.G., da Fonseca G.A.B & Kent J. 2000. Biodiversity hotspots for conservation priorities. Nature 403: 853–858.

Naseer A., Ghani S., Niazi A.R. & Khalid A.N. 2019. Inocybe caroticolor from oak forests of Pakistan. Mycotaxon 134 (2): 241‒251.

Saba M., Haelewaters D., Pfister D.H. & Khalid A.N. 2020. New species of Pseudosperma (Agaricales, Inocybaceae) from Pakistan revealed by morphology and multi-locus phylogenetic reconstruction. MycoKeys 69: 1–31.

Tamura K., Stecher G. & Kumar S. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38 (7): 3022–3027.

Voytas D. 2001. Agarose gel electrophoresis. Current protocols in molecular biology 51 (1): 2‒5.

World Weather Information Service. 2018. World meteorological organization. Available from [accessed 31 Jun. 2022].

Yu W.-J., Chang C., Qin L.-W., Zeng N.-K., Wang S.-X. & Fan Y.-G. 2020. Pseudosperma citrinostipes (Inocybaceae), a new species associated with Keteleeria from southwestern China. Phytotaxa 450 (1): 8–16.

How to Cite
Razzaq, A., Naseer, A., & Khalid, A. N. (2023). Inocybe subhimalayanensis (Agaricales, Inocybaceae), a new smooth spored Inocybe species from Pakistan. European Journal of Taxonomy, 870(1), 76-86.
Research article