Lactarius indohirtipes and L. sharmai (Russulales, Basidiomycota): two novel species from Jammu and Kashmir, India

Keywords: macrofungi, nrITS, phylogeny, Russulaceae, taxonomy

Abstract

Exploration of macrofungi in the Jammu and Kashmir regions, India, led to the discovery of two novel species, described herein as Lactarius indohirtipes sp. nov. and L. sharmai sp. nov. This paper presents detailed morphological descriptions and illustrations, as well as a phylogenetic analysis based on nrITS sequences. A comparative analysis with related species is also included.

References

Altaf U., Verma K., Ghosh A., Mehmood T. & Sharma Y.P. 2022. A new species of genus Russula subsect. Ilicinae (Russulaceae) from Kashmir Himalaya based on morphology and molecular phylogeny. Nordic Journal of Botany 2022. https://doi.org/10.1111/njb.03452

Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W. & Lipman D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25 (17): 3389–3402. https://doi.org/10.1093/nar/25.17.3389

Atri N.S., Sharma S., Saini M.K. & Das K. 2016. Researches on russulaceous mushrooms – An appraisal. Kavaka 47: 63–82.

Bera I. & Das K. 2021. Morphology and molecular phylogeny reveal three new records of wild mushrooms from India in Lactarius subgenus Russularia. Nelumbo 63 (1): 183–196. https://doi.org/10.20324/nelumbo/v63/2021/157371

Bera I. & Das K. 2023. Two new species of genus Lactarius Pers. (Russulaceae) from Arunachal Pradesh, India. Cryptogamie, Mycologie 44 (11): 147–158. https://doi.org/10.5252/cryptogamie-mycologie2023v44a11

Buyck B., Hofstetter V., Verbeken A. & Walleyn R. 2010. Proposal to conserve Lactarius nom. cons. (Basidiomycota) with a conserved type. Taxon 59 (1): 295–296. https://doi.org/10.1002/tax.591031

Clark K., Karsch-Mizrachi I., Lipman D.J., Ostell J. & Sayers E.W. 2016. GenBank. (Database issue): D67–D72. Nucleic Acids Research 44 (1). https://doi.org/10.1093/nar/gkv1276

Das K. & Chakraborty D. 2014. Lactarius vesterholtii, a new species from India. Mycotaxon 129 (2): 477–484. https://doi.org/10.5248/129.477

Das K. & Sharma J.R. 2005. Russulaceae of Kumaon Himalaya. Botanical Survey of India, Ministry of Environment and Forests, Govt. of India, Kolkata.

Das K., Verbeken A., Chakraborty D., Avchar R. & Baghela A. 2017. Morphological and phylogenetic evidence for two new Lactarius (Russulales, Basidiomycota) species from India. Cryptogamie, Mycologie 38 (4): 453–467. https://doi.org/10.7872/crym/v38.iss4.2017.453

Das K., Bera I. & Ghosh A. 2020. Lactarius brunneoaurantiacus sp. nov. and L. indoscrobiculatus sp. nov. from India. Nordic Journal of Botany 38 (11): e02940. https://doi.org/10.1111/njb.02940

De Crop E., Nuytinck J., Van de Putte K., Wisitrassameewong K., Hackel J., Stubbe D., Hyde K.D., Roy M., Halling R.E., Moreau P.A., Eberhardt U. & Verbeken A. 2017. A multi-gene phylogeny of Lactifluus (Basidiomycota, Russulales) translated into a new infrageneric classification of the genus. Persoonia 38: 58–80. https://doi.org/10.3767/003158517X693255

Edler D., Klein J., Antonelli A. & Silvestro D. 2021. raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods in Ecology and Evolution 12 (2): 373–377. https://doi.org/10.1111/2041-210X.13512

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series 41: 95–98.

Heilmann-Clausen J., Verbeken A. & Vesterholt J. 1998. The Genus Lactarius. Fungi of Northern Europe, Vol. 2. Svampertryk, Mundelstrup.

Kalichman J., Kirk P.M. & Matheny P.B. 2020. A compendium of generic names of agarics and Agaricales. Taxon 69 (3): 425–447. https://doi.org/10.1002/tax.12240

Katoh K. & Standley D.M. 2013. MAFFT multiple sequence alignment software ver. 7: improvement in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010

Kornerup A. & Wanscher J.H. 1978. Methuen Handbook of Colour, 3rd Edition. Eyre Methuen Ltd., London.

Kumar A., Sharma Y.P., Verma K. & Mehmood T. 2021. Amanita parvirufobrunnescens (Agaricales: Amanitaceae), a new species in A. sect. Amidella from India. Nordic Journal of Botany 39 (4): e03141. https://doi.org/10.1111/njb.03141

Largent D., Johnson D. & Watling R. 1977. How to Identify Mushrooms to Genus III: Microscopic Features. Mad River Press Inc.

Le H.T., Stubbe D., Verbeken A., Nuytinck J., Lumyong S. & Desjardin D.E. 2007. Lactarius in Northern Thailand: 2. Lactarius subgenus Plinthogali. Fungal Diversity 27: 61–94.

Lee H., Park M.S., Jung P.E., Jonathan J.F., Oh S.Y., Verbeken A. & Lim Y.W. 2015. Lactarius cucurbitoides (Russulales, Basidiomycota), a new species from South Korea supported by molecular and morphological data. Phytotaxa 205 (3): 168–176.

Paloi S., Verbeken A. & Acharya K. 2019. Lactarius brunneocinnamomeus, a new species of Lactarius subgenus Russularia from West Bengal, India. Phytotaxa 416 (4): 294–300. https://doi.org/10.11646/phytotaxa.416.4.8

Stubbe D. & Verbeken A. 2012. Lactarius subg. Plinthogalus: the European taxa and American varieties of L. lignyotus re-evaluated. Mycologia 104 (6): 1490–1501. https://doi.org/10.3852/12-039

Uniyal P., Das K. & Bhatt R.P. 2018. Two novel species of Lactarius subgenus Plinthogalus from Uttarakhand Himalaya. Phytotaxa 338 (3): 255–264. https://doi.org/10.11646/phytotaxa.338.3.3

Uniyal P., Das K. & Bhatt R.P. 2019. Lactarius pleuromacrocystidiatus (Russulaceae), a novel species from India. Kew Bulletin 74: 8. https://doi.org/10.1007/s12225-019-9799-0

Vellinga E.C. 1988. Glossary. In: Bas C., Kuyper T.W., Noordeloos M.E. & Vellinga E.C. (eds) Flora Agaricina Neerlandica, Vol. 1: 54–64. A.A. Balkema, Rotterdam.

Verbeken A., Horak E. & Desjardin D.E. 2001. Agaricales of Indonesia. 3. New records of the genus Lactarius (Basidiomycetes, Russulales) from Java. Sydowia 53: 261–289.

Verma K., Mehmood T., Uniyal P., Kapoor R. & Sharma Y.P. 2021. Two new species of genus Lactarius (Russulaceae) from Northwestern Himalaya, India. Phytotaxa 500 (4): 253–265. https://doi.org/10.11646/phytotaxa.500.4.1

Verma K., Mehmood T., Uniyal P. & Sharma Y.P. 2022. Lactarius indoevosmus and L. kanadii (Russulaceae), two new species from the northwestern Himalayas, India, inferred from morphology and molecular data. Phytotaxa 541: 165–177. https://doi.org/10.11646/phytotaxa.541.2.6

Verma K., Kumar A., Uniyal P., Mehmood T. & Sharma Y.P. 2023. Lactarius bhaderwahensis: a new species of Lactarius subg. Plinthogalus (Russulaceae) from coniferous forests of North western Himalayas, India. Nordic Journal of Botany 2023 (4): e03791. https://doi.org/10.1111/njb.03791

Wang X.H. 2017. Two new species of Lactarius subg. Russularia from subalpine regions of southwestern China. Journal of Fungal Research 15 (4): 222–228.

Wang X.H. 2018. Fungal biodiversity profiles 71–80. Cryptogamie, Mycologie 39 (4): 419–445. https://doi.org/10.7872/crym/v39.iss4.2018.419

Wang X.H. & Liu P.G. 2002. Lactarius chichuensis and L. hirtipes, two easily confused species. Mycotaxon 84: 391–400.

Wang X.H., Das K., Bera I., Chen Y.H., Bhatt R.P., Ghosh A., Hembrom M.E., Hofstetter V., Parihar A., Vizzini A., Xu T.M., Zhao C.L. & Buyck B. 2019. Fungal biodiversity profiles 81–90. Cryptogamie, Mycologie 40 (5): 57–95. https://doi.org/10.5252/cryptogamie-mycologie2019v40a5

White T.J., Bruns T., Lee S. & Taylor J. 1990. 38 – Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A, Gelfand D.H., Sninsky J.J. & White T.J. (eds) PCR Protocols: a Guide to Methods and Applications: 315–322. Academic Press, San Diego, New York. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Wisitrassameewong K., Nuytinck J., Hampe F., Hyde K.D. & Verbeken A. 2014. Lactarius subgenus Russularia (Russulaceae) in South-East Asia: 2. Species with remarkably small basidiocarps. Phytotaxa 188 (4): 181–197. https://doi.org/10.11646/phytotaxa.188.4.1

Wisitrassameewong K., Nuytinck J., Le H.T., De Crop E., Hampe F., Hyde K.D. & Verbeken A. 2015. Lactarius subgenus Russularia (Russulaceae) in South-East Asia: 3. New diversity in Thailand and Vietnam. Phytotaxa 207 (3): 215–241. https://doi.org/10.11646/phytotaxa.207.3.1

Wisitrassameewong K., Looney B.P., Le H.T., De Crop E., Das K., Van de Putte K., Eberhardt U., Jiayu G., Stubbe D., Hyde K.D., Verbeken A. & Nuytinck J. 2016. Lactarius subgenus Russularia (Basidiomycota, Russulales): novel Asian species, worldwide phylogeny and evolutionary relationships. Fungal Biology 120 (12): 1554–1581. https://doi.org/10.1016/j.funbio.2016.08.004

Published
2024-08-13
How to Cite
Verma, K., Ahamed, M., Kumar, A., Mehmood, T., & Uniyal, P. (2024). Lactarius indohirtipes and L. sharmai (Russulales, Basidiomycota): two novel species from Jammu and Kashmir, India. European Journal of Taxonomy, 947(1), 175-190. https://doi.org/10.5852/ejt.2024.947.2627
Section
Research article