Integrative description of two new species of the Xenylla maritima species complex (Collembola: Hypogastruridae) from Crete (Greece), with notes on the high intraspecific genetic divergence found in X. maritima Tullberg, 1869

  • Dariusz Skarżyński University of Wrocław, Faculty of Biological Sciences, Department of Invertebrate Biology, Evolution and Conservation, Przybyszewskiego 65, 51-148 Wrocław, Poland https://orcid.org/0000-0002-1767-5747
  • Barbara Valle Department of Life Sciences, Università degli Studi di Siena, Via A. Moro 2, 53100 Siena, Italy; NBFC, National Biodiversity Future Center, Palermo, Italy https://orcid.org/0000-0003-4829-4776
  • Dariusz J. Gwiazdowicz  Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Forest Entomology and Pathology, Wojska Polskiego 71c, 60-625, Poznań, Poland https://orcid.org/0000-0002-0064-2316
  • David Porco Musée National d’Histoire Naturelle du Luxembourg, 24, rue Münster, L-2160 Luxembourg, Luxembourg https://orcid.org/0000-0003-1914-1973
Keywords: DNA barcoding, Europe, overlooked diversity, Xenylla ellisi sp. nov., Xenylla schulzi sp. nov.

Abstract

A classical taxonomic analysis of materials from several European museums as well as our own collection along with the analysis of DNA barcoding data (cox1) allowed us to determine the taxonomic status of Cretan forms included in the recently revised Xenylla maritima complex. Two of them are recognized as distinct species: Xenylla ellisi sp. nov. and Xenylla schulzi sp. nov., and a new cryptic lineage was found within the morphological boundaries of the broadly distributed species X. maritima

References

Babenko A.B., Chernova N.M., Potapov M.B. & Stebaeva S.K. 1994. Collembola of Russia and Adjacent Countries. Family Hypogastruridae. Nauka, Moscow.

Bellinger P.F., Christiansen K.A. & Janssens F. 1996–2024. Checklist of the Collembola of the World. Available from http://www.collembola.org [accessed 3 Jun. 2024].

Benchling [Biology Software]. (2024). Available from https://benchling.com [accessed 8 Nov. 2024].

Deharveng L. 1983. Morphologie évolutive des Collemboles Neanurinae en particulier de la lignée Neanurienne. Travaux du Laboratoire d’Ecobiologie des Arthropodes edaphiques, Toulouse 4: 1–63.

Ellis W.N. 1976. Autumn fauna of Collembola from Central Crete. Tijdschrift voor Entomologie 119: 221–326.

Fjellberg A. 1984. Maxillary structures in Hypogastruridae (Collembola). Annales de la Société royale zoologique de Belgique 114: 89–99.

Fjellberg A. 1998. The Collembola of Fennoscandia and Denmark, Part 1: Poduromorpha. Fauna Entomologica Scandinavica vol. 35. Brill, Leiden / Boston / Köln. https://doi.org/10.1163/9789004273634

Fjellberg A. 1999. The labial palp in Collembola. Zoologischer Anzeiger 237: 309–330.

Folmer O., Black M., Hoeh W., Lutz R. & Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3 (5): 294–299.

Gama M.M. da. 1988. Filogenia des espécies de Xenylla à escala mundial (Insecta, Collembola). Evolución biológica, Coimbra 2: 139–147.

Gwiazdowicz D.J., Skarżyński D., Fazan L., Fragnière Y., Ghosn D., Kozlowski G., Kuźmiński R., Remoundou E. & Zawieja B. 2022. Microarthropods living on the endemic tree Zelkova abelicea (Ulmaceae) with particular attention to Collembola diversity. Forests 13 (2): e195. https://doi.org/10.3390/f13020195

Hopkin S.P. 1997. Biology of the Springtails (Insecta: Collembola). Oxford University Press, London. https://doi.org/10.1093/oso/9780198540847.001.0001

Hüther W. 1962. Beitrag zur Gattung Willemia Börner (Collembola). Beiträge zur Entomologie 12: 511–526.

Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide-sequences. Journal of Molecular Evolution 16 (2): 111–120. https://doi.org/10.1007/BF01731581

Kumar S., Stecher G. & Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33 (7): 1870–1874. https://doi.org/10.1093/molbev/msw054

Larsson A. 2014. AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30 (22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531

Lawrence P.N. 1977. Studies on the tibiotarsal chaetotaxy of Collembola. Systematic Entomology 2: 313–317. https://doi.org/10.1111/j.1365-3113.1977.tb00380.x

Massoud Z. 1967. Monographie des Neanuridae, Collemboles Poduromorphes à pièces buccales modifiées. In: Delamare Debouteville C. & Rapoport E.H. (eds) Biologie de l’Amérique Australe 3: 7–399. CNRS, Paris.

Pomorski R.J. & Skarżyński D. 1997. A redescription of Metaphorura denisi Simon Benito, 1985 (Collembola, Onychiuridae, Tullbergiinae). Genus 8 (3–4): 489–496.

Porco D., Bedos A., Greenslade P., Janion C., Skarżyński D., Stevens M.I., Jansen van Vuuren B. & Deharveng L. 2012a. Challenging species delimitation in Collembola: cryptic diversity among common springtails unveiled by DNA barcoding. Invertebrate Systematics 26 (6): 470–477. https://doi.org/10.1071/is12026

Porco D., Potapov M., Bedos A., Busmachiu G., Weiner W.M., Hamra-Kroua S. & Deharveng L. 2012b. Cryptic diversity in the ubiquist species Parisotoma notabilis (Collembola, Isotomidae): a long-used chimeric species? PLoS ONE 7 (9): e46056. https://doi.org/10.1371/journal.pone.0046056

Porco D., Skarżyński D., Decaens T., Hebert P.D.N. & Deharveng L. 2014. Barcoding the Collembola of Churchill: a molecular taxonomic reassessment of species diversity in a sub-Arctic area. Molecular Ecology Resources 14: 249–261. https://doi.org/10.1111/1755-0998.12172

Rackham O. & Moody J. 1996. The Making of the Cretan Landscape. Manchester University Press, Manchester / New York.

Saitou N. & Nei M. 1987. The neighbor-joining method – a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4 (4): 406–425.

Schulz H.-J. 2007. Second contribution to the knowledge of the springtail fauna of the White Mountains (Lefká Óri) in West Crete (Insecta: Collembola). Mitteilungen des internationalen entomologischen Vereins 32: 175–183.

Schulz H.-J. 2010. A contribution to the Collembola fauna of Crete (Insecta: Collembola). Mitteilungen des internationalen entomologischen Vereins 35: 101–110.

Schulz H.-J. & Burkhardt U. 2012. Interessante Collembolennachweise aus Ostkreta (Hexapoda, Collembola). Linzer biologische Beiträge 44 (2): 1669–1676.

Schulz H.-J. & Lymberakis P. 2006. First contribution to the knowledge of the Collembola fauna of the White Mountains (Lefká Óri) in West Crete (Insecta, Collembola). Senckenbergiana Biologica 86: 229–234.

Sfenthourakis S. & Triantis K.A. 2017. The Aegean archipelago: a natural laboratory of evolution, ecology and civilisations. Journal of Biological Research, Thessaloniki 24: e4. https://doi.org/10.1186/s40709-017-0061-3

Simon Benito J.C. 1985. Colémbolos de suelos de sabinar en la provincia de Guadalajara. Eos 61: 293–318.

Skarżyński D. & Gwiazdowicz D.J. 2022. A redescription of Hypogastrura gisini Strenzke, 1954 (Collembola: Hypogastruridae) and description of a new related species from Crete (Greece). Zootaxa 5155 (1): 52–60. https://doi.org/10.11646/zootaxa.5155.1.2

Skarżyński D., Kahrarian M., Piwnik A. & Zawisza M. 2017. Description of Hypogastrura ellisi sp. n. (Collembola, Hypogastruridae), with notes on H. tethyca Ellis and the trybomi group. ZooKeys 719: 45–57. https://doi.org/10.3897/zookeys.719.14806

Skarżyński D., Piwnik A. & Porco D. 2018. Integrating morphology and DNA barcodes for species delimitation within the species complex Xenylla maritima (Collembola: Hypogastruridae). Arthropod Systematics & Phylogeny 76 (1): 31–43. https://doi.org/10.3897/asp.76.e31935

Smolis A. & Kaprus’ I.J. 2009. Redescription of Endonura cretensis (Ellis, 1976) (Collembola: Neanuridae: Neanurinae) with a new record from Israel. Annales Zoologici 59: 1–6. https://doi.org/10.3161/000345409X432538

Smolis A., Skarżyński D. & Gwiazdowicz D.J. 2023. New species of Neanuridae (Collembola) living on the endemic tree Zelkova abelicea in Crete. Zootaxa 5278 (2): 333–350. https://doi.org/10.11646/zootaxa.5278.2.6

Spiliopoulou K., Dimitrakopoulos P.G., Brooks T.M., Kelaidi G., Paragamian K., Kati V., Oikonomou A., Vavylis D., Trigas P., Lymberakis P., Darwall W., Stoumboudi M.T. & Triantis K.A. 2021. The Natura 2000 network and the ranges of threatened species in Greece. Biodiversity Conservation 30: 945–961. https://doi.org/10.1007/s10531-021-02125-7

Sterzyńska M. & Ehrnsberger R. 1997. Marine algae wrack Collembola of European coasts. Abhandlungen und Berichte des Naturkundemuseums Görlitz 69 (2): 165–178.

Thibaud J.-M., Schulz H.-J. & Gama M.M. da. 2004. Synopses on Palearctic Collembola. Hypogastruridae. Vol. 4. Abhandlungen und Berichte des Naturkundemuseums Görlitz 75 (2): 1–287.

Xu S., Li L., Luo X., Chen M., Tang W., Zhan L., Dai Z., Lam T.T., Guan Y. & Yu G. 2022. Ggtree: A serialized data object for visualization of a phylogenetic tree and annotation data. iMeta: e56. https://doi.org/10.1002/imt2.56

Published
2025-01-30
How to Cite
Skarżyński, D., Valle, B., Gwiazdowicz, D. J., & Porco, D. (2025). Integrative description of two new species of the Xenylla maritima species complex (Collembola: Hypogastruridae) from Crete (Greece), with notes on the high intraspecific genetic divergence found in X. maritima Tullberg, 1869. European Journal of Taxonomy, 976(1), 77–91. https://doi.org/10.5852/ejt.2025.976.2777
Section
Research article