Unexpected discovery of six new species of Aphyosemion (Cyprinodontiformes, Aplocheilidae) in the Wonga-Wongué Presidential Reserve in Gabon

  • Jean-François Agnèse  ISEM, CNRS, Univ. Montpellier, IRD, EPHE, Montpellier
  • Laurent Chirio 14, rue des Roses, 06130 Grasse
  • Olivier Legros Floralaan 51, 1501 Buizingen
  • Richard Oslisly Patrimoines Locaux et Gouvernance UMR 208, IRD, MNHN, Paris
  • Hervé Mvé Bhé Institut de Recherches Agronomiques et Forestières (IRAF), Centre National de la Recherche Scientifique et Technologique (CENAREST), BP 2246, Libreville
Keywords: Killifish, mtDNA, Nothobranchiidae, taxonomy

Abstract

During a survey of the fishes in the region of the Wonga-Wongué Presidential Reserve, 14 new populations of the subgenus Chromaphyosemion Myers, 1924 were found. These observations extend the previously known distribution range of the subgenus 120 kilometres southward. None of these populations could be related to any described species. Based on the colouration of the males and females, together with a genetic marker (mitochondrial DNA cytochrome b sequences), the populations studied are grouped into six new species which are described in this article, all close to Aphyosemion alpha Huber, 1998 with which they share the presence of a black alpha-shaped mark on the pre- and post-opercular region. The group composed of A. alpha and the six new species is referred to here as the A. alpha species group. All the new species, A. aurantiacum Chirio, Legros & Agnèse sp. nov., A. barakoniense Chirio, Legros & Agnèse sp. nov., A. flammulatum Chirio, Legros & Agnèse sp. nov., A. flavocyaneum Chirio, Legros & Agnèse sp. nov., A. pusillum Chirio, Legros & Agnèse sp. nov. and A. rubrogaster Chirio, Legros & Agnèse sp. nov., are further unambiguously diagnosed by unique combinations of colour patterns, making it possible to generate an identification key for the A. alpha species group. It is likely that the coastal dunes of Wonga-Wongué that form a sandy relief, could have led to the fragmentation and then isolation of the hydrographical networks that flow into the Atlantic Ocean, making possible a significant number of allopatric speciations.

References

Agnèse J.-F., Zentz F., Legros O. & Sellos D. 2006. Phylogenetic relationships and phylogeography of the killifish species of the subgenus Chromaphyosemion (Radda, 1971) in West Africa, inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution 40: 332–346. https://doi.org/10.1016/j.ympev.2006.03.018

Agnèse J.-F., Legros O., Cazaux B. & Estivals G. 2013. Aphyosemion pamaense, a new killifish species (Cyprinodontiformes: Nothobranchiidae) from Cameroon. Zootaxa 3670 (4): 516–530. https://doi.org/10.11646/zootaxa.3670.4.6

Antoine P., Lautridou J.-P. & Laurent M. 2000. Long-term fluvial archives in NW France: response of the Seine and Somme Rivers to tectonic movements, climatic variations and sea-level changes. Geomorphology 33: 183–207. https://doi.org/10.1016/S0169-555X(99)00122-1

Collier G. 2006. The genus Aphyosemion: taxonomic history and molecular phylogeny. Journal of the American Killifish Association 39 (5–6) : 147–168.

Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791. https://doi.org/10.2307/2408678

Huber J.H. 2000. Killi-Data 2000. Updated Checklist of taxonomic Names, collecting Localities and bibliographic References of oviparous Cyprinodont Fishes (Cyprinodontiformes); in French, English, German and Spanish. Société française d’Ichtyologie, Paris.

Huber J.H. 2006. Killi-Data Catalogue. Paris, France.

ICZN. 1999. International Code of Zoological Nomenclature. Fourth Edition. The International Trust for Zoological Nomenclature, London.

Kimura M. 1980. A simple model for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120. https://doi.org/10.1007/bf01731581

Kullmann H. & Klemme B. 2007. Female mating preference for own males on species and population level in Chromaphyosemion killifishes (Cyprinodontiformes, Nothobranchiidae). Zoology 110: 377–386. https://doi.org/10.1016/j.zool.2007.08.001

Legros O., Zentz F. & Agnèse J.-F. 2005. Description de deux nouveaux Chromaphyosemion (Teleostei: Aplocheilidae) du sud de la Plaine littorale camerounaise. Killi-Contact 33: 3–30.

Nei M., Kumar S. & Takahashi K. 1998. The optimization principle in phylogenetic analysis tends to give incorrect topologies when the number of nucleotides or amino acids used is small. Proceedings of National Academy of Sciences 95: 12390–12397. https://doi.org/10.1073/pnas.95.21.12390

Saitou N. & Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454

Sambrook J., Fritsch E.F. & Maniatis T. 1989. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.

Scheel J.J. 1968. Rivulins of the Old World. TFH publications, New Jersey.

Scheel J.J. 1990. Atlas of Killifishes of the Old World. TFH publications, New Jersey.

Schwarz G. 1978. Estimating the dimension of a model. Annals of Statistics 6 (2): 461–464. https://doi.org/10.1214/aos/1176344136

Sonnenberg R. 2000. The distribution of Chromaphyosemion Radda, 1971 (Teleostei: Cyprinodontiformes) on the Coastal Plains of West and Central Africa. In: Rheinwald G. (ed.) Isolated Vertebrate Communities in the Tropics. Proceedings of the 4th International Symposium, Bonn. Bonner zoologische Monographien 46: 79–94.

Sonnenberg R. 2007a. Two new species of Chromaphyosemion (Cyprinodontiformes: Nothobranchiidae) from the coastal plain of Equatorial Guinea. Ichthyological Exploration of Freshwaters 18: 359–373.

Sonnenberg R. 2007b. Description of three new species of the genus Chromaphyosemion Radda, 1971 (Cyprinodontiformes: Nothobranchiidae) from the coastal plains of Cameroon with a preliminary review of the Chromaphyosemion splendopleure complex. Zootaxa 1591: 1–38.

Stamatakis A. 2014. RAxML Version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033

Takahashi K. & Nei M. 2000. Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Molecular Biology and Evolution 17: 1251–1258. https://doi.org/10.1093/oxfordjournals.molbev.a026408

Tamura K. & Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10 (3): 512–526.https://doi.org/10.1093/oxfordjournals.molbev.a040023

Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731–2739. https://doi.org/10.1093/molbev/msr121

UngV., Dubus G., Zaragüeta Bagils R. & Vignes-Lebbe R. 2010. Xper2: introducing e-taxonomy. Bioinformatics 26 (5): 703–704. https://doi.org/10.1093/bioinformatics/btp715

Van Handel T.H. 1989. Late Quaternary sea-level changes and archaeology. Antiquity 63: 733–745.

Völker M.E. 2006. Karyotype Differentiation in Chromaphyosemion Killifishes (Cyprinodontiformes, Nothobranchiidae): Patterns, Mechanisms and Evolutionary Implications. PhD Thesis, University of Bonn, Germany.

Wildekamp R.H. 1993. A World of Killies. Atlas of the Oviparous Cyprinodontiform Fishes of the World. Volume 1. The American Killifish Association, Mishawaka, Indiana.

Zhang J., Kapli P., Pavlidis P. & Stamatakis A. 2013. A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29: 2869–2876. https://doi.org/10.1093/bioinformatics/btt499

Published
2018-10-30
How to Cite
Agnèse, J.-F., Chirio, L., Legros, O., Oslisly, R., & Bhé, H. M. (2018). Unexpected discovery of six new species of Aphyosemion (Cyprinodontiformes, Aplocheilidae) in the Wonga-Wongué Presidential Reserve in Gabon. European Journal of Taxonomy, (471). https://doi.org/10.5852/ejt.2018.471