Contribution to chromosome numbers and phylogeny of Turkish Vincetoxicum Wolf (Apocynaceae, Asclepiadoideae)

Seher Güven, Serdar Makbul, Kamil Coşkunçelebì

Abstract


We report chromosome counts for ten taxa of Vincetoxicum sensu stricto (s. str.) (Apocynaceae) from Turkey (of which two are endemic), including the first chromosome counts for V. canescens subsp. pedunculata, V. funebre, V. fuscatum subsp. boissieri, V. parviflorum and V. tmoleum. Two taxa of V. fuscatum proved to be tetraploid (2n=44) and the remaining eight taxa diploid (2n=22). Molecular phylogenetic analyses based on nrDNA (ITS) and cpDNA (trnT-trnL) (including 31 newly generated sequences) confirm the position of the Turkish Vincetoxicum in the Vincetoxicum s. str. clade. Vincetoxicum fuscatum, V. parviflorum, V. speciosum, as well as the Turkish endemic V. fuscatum subsp. boissieri, were clearly resolved as species-level clades, whereas the delimitation of the rest of the Turkish taxa was less clear based on molecular data.


Keywords


Cytology; molecular phylogeny; Turkey; Vincetoxicum

Full Text:

PDF PDF/A


DOI: https://doi.org/10.5852/ejt.2019.590

References


Ali S.I. 1983. Asclepiadaceae. In: Nasir E. & Ali S.I. (eds) Flora of Pakistan-Karachi. Vol. 150: 1–165. University of Karachi Press, Pakistan.

Altundağ E. & Öztürk M. 2011. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Social and Behavioral Sciences 19: 756–777. https://doi.org/10.1016/j.sbspro.2011.05.195

Aparicio A. & Silvestre S. 1985. Números cromosómicos para la flora Española. Lagascalia 13 (2): 318–323.

Boissier E.P. 1875. Vincetoxicum Maench. In: Boissier E.P. (ed.) Flora Orientalis: 51–56. H. Georg, Basileae, Genevae.

Browicz K. 1975. Vincetoxicum canescens (Willd.) Decne. and V. tmoleum Boiss. Systematic problems and geographical distribution. Fragmenta Floristica et Geobotanica 21: 261–271.

Browicz K. 1978. Vincetoxicum Wolf. In: Davis P.H. (ed.) Flora of Turkey and East Aegean Islands. Vol. 7: 167–173. Edinburgh University Press, Edinburgh.

Chang Y.H., Tzeng H.Y. & Tseng Y.H. 2012. Pollinaria morphology of Cynanchum L. (Asclepiadaceae) in Taiwan. Quarterly Journal of Forestry Research 34 (4): 251–262.

Civeyrel L., Thomas A.L., Ferguson K. & Chase M.W. 1998. Critical reexamination of palynological characters used to delimit Asclepiadaceae in comparison to the molecular phylogeny obtained from plastid matK sequences. Molecular Phylogenetics and Evolution 9 (3): 517–527.

https://doi.org/10.1006/mpev.1997.0510

DiTommaso A., Lawlor F.M. & Darbyshire S.J. 2005. The biology of invasive alien plants in Canada, 2, Cynanchum rossicum (Kleopow) Borhidi [=Vincetoxicum rossicum (Kleopow) Barbar.] and Cynanchum louiseae (L.) Kartesz & Gandhi [= Vincetoxicum nigrum (L.) Moench]. Canadian Journal of Plant Science 85: 243–263.

Doğan A. 2008. Ovacık-Tunceli yöresinin geleneksel halk ilacı olarak kullanılan bitkileri. Master’s thesis, Marmara Üniversitesi, Istanbul.

Domin K. 1928. Beiträge zur flora und pflanzengeographie Australiens. Bibliotheca Botanica 22 (89): 1033–1128.

Doyle J.J. & Dolye J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

Ekim T., Koyuncu M., Vural M., Duman H., Aytaç Z. & Adıgüzel N. 2000. Türkiye Bitkileri Kırmızı Kitabı (Eğrelti ve Tohumlu Bitkiler). Doğal Hayatı Koruma Derneği, Ankara.

Elçi Ş. 1994. Sitogenetikte araştırma yöntemleri ve gözlemler. 100. Yıl Üniversitesi Yayınları, Van.

Feng Y., Qin X., Huang J. & Hong W. 2012. Pollinarium morphology of 17 species and 1 subspecies of Cynanchum (Asclepiadaceae). Acta Botanica Boreali-Occidentalia Sinica 32 (9): 1758–1762.

Forster P.I. 1991. A taxonomic revision of Cynanchum L. (Asclepiadaceae: Asclepiadoideae) in Australia. Austrobaileya 3: 443–466.

Garbari F., Bedini G. & Peruzzi L. 2012. Chromosome numbers of the Italian flora. From the Caryologia foundation to present. Caryologia 65 (1): 62–71. https://doi.org/10.1080/00087114.2012.678090

Gaynor M.L., Ng J. & Laport L.G. 2018. Phylogenetic structure of plant communities: Are polyploids distantly related to co-occurring diploids? Frontiers in Ecology and Evolution 6: 52.

https://doi.org/10.3389/fevo.2018.00052

Goyder D., Nicholas A. & Liede-Schumann S. 2007. Phylogenetic relationships in subtribe Asclepiadinae (Apocynaceae:Asclepiadoideae). Annals of the Missouri Botanical Garden 94: 423–434.

https://doi.org/10.3417/0026-6493(2007)94[423:PRISAA]2.0.CO;2

Gregg W.C.T., Ather S.H. & Hahn M.W. 2017. Gene-Tree reconciliation with MUL-Trees to resolve polyploidy events. Systematic Biology 66 (6): 1007–1018. https://doi.org/10.1093/sysbio/syx044

Grossheim A.A. 1967. Flora of the Caucasus. 7. Academy of Sciences Press, Moscow/Leningrad.

Gültepe M., Uzuner U., Coşkunçelebi K., Beldüz A.O. & Terzioğlu S. 2010. Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany 34: 147–157. https://doi.org/10.3906/bot-0905-23

Güner A. 2012. Vincetoxicum Wolf. In: Güner A., Aslan S., Ekim T., Vural M. & Babaç M.T. (eds) Türkiye bitkileri listesi (Damarlı bitkiler): 85–86. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını.

Güven S. 2017. Türkiye Vincetoxicum (Apocynaceae Asclepiadoideae) cinsinin taksonomik revizyonu. PhD Thesis, Recep Tayyip Erdoğan Üniversitesi, Rize.

Güven S., Makbul S., Coskuncelebi K. & Pınar N.M. 2015. Pollinarium morphology of the genus Vincetoxicum Wolf (Apocynaceae: Asclepiadoideae) in Turkey. Phytotaxa 230 (1): 22–38.

https://doi.org/10.11646/phytotaxa.230.1.2

Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Research 41: 95–98.

İlçim A., Özay S.G. & Kökdil G. 2010. Exomorphic seed characters and anatomy of leaf and stem of some Vincetoxicum (Asclepiadaceae/Apocynaceae) species from Turkey. Journal of Faculty of Pharmacy of Ankara University 39 (1): 1–16. https://doi.org/10.1501/Eczfak_0000000550

Jones R.N. & Rickards G.K. 1991. Practical genetics. Open University Press, Philadelphia.

Kumar S., Stecher G. & Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 (7): 1870–1874.

https://doi.org/10.1093/molbev/msw054

Lahaye R., Civeyrel L., Speck T. & Rowe N.P. 2005. Evolution of shrub-like growth forms in the lianoid subfamily Secamonoideae (Apocynaceae s.l.) of Madagascar: phylogeny, biomechanics, and development. American Journal of Botany 92 (8): 1381–1396. https://doi.org/10.3732/ajb.92.8.1381

Leliaert F., Verbruggen H., Vanormelingen P., Steen F., López-Bautista J.M., Zuccarello G.C. & De Clerck O. 2014. DNA-based species delimitation in algae. European Journal of Phycology 49: 179–196.

https://doi.org/10.1080/09670262.2014.904524

Lessani H. & Chariat-Panahi S. 1979. In: Löve A. (ed.) IOPB chromosome number reports LXV: 635–636. Taxon 28 (4): 627–637.

Li P.T., Gilbert M.G. & Stevens W.D. 1995. Asclepiadaceae. In: Wu Z.Y. & Raven P.H. (eds) Flora of China. Vol. 16: 189–270. Missouri Botanical Garden Press, St. Louis.

Liede S. 1996. Cynanchum–Rhodostegiella–Vincetoxicum–Tylophora (Asclepiadaceae): new considerations on an old problem. Taxon 45: 193–211. https://doi.org/10.2307/1224660

Liede S. 2001. Subtribe Astephaninae (Apocynaceae-Asclepiadoideae) reconsidered: new evidence based on cpDNA spacers. Annals of the Missouri Botanical Garden 88 (4): 657–668.

https://doi.org/10.2307/3298638

Liede S. & Täuber A. 2002. Circumscription of the genus Cynanchum (Apocynaceae–Asclepiadoideae). Systematic Botany 27(4): 789–800.

Liede S., Täuber A. & Schneidt J. 2002. Molecular considerations in the Tylophorinae K.Schum. (Apocynaceae-Asclepiadoideae). Edinburgh Journal of Botany 59 (3): 377–403.

https://doi.org/10.1017/S0960428602000239

Liede-Schumann S. & Meve U. 2018. Vincetoxicum (Apocynaceae–Asclepiadoideae) expanded to include Tylophora and allies. Phytotaxa 369 (3): 129–184. https://doi.org/10.11646/phytotaxa.369.3.1

Liede-Schumann S., Kong H., Meve U. & Thiv M. 2012. Vincetoxicum and Tylophora (Apocynaceae:Asclepiadoideae:Asclepiadeae) – two sides of the same medal: independent shifts from tropical to temperate habitats. Taxon 61 (4): 803–825. https://doi.org/10.1002/tax.614007

Liede-Schumann S., Khanum R., Mumtaz A.S., Gherghel I. & Pahlevani A. 2016. Going west – a subtropical lineage (Vincetoxicum, Apocynaceae: Asclepiadoideae) expanding into Europe. Molecular Phylogenetics and Evolution 94: 436–446. https://doi.org/10.1016/j.ympev.2015.09.021

Mansoor A., Ibrahim M.A., Zaidi M.A. & Ahmed M. 2011. Antiprotozoal activities of Vincetoxicum stocksii and Carum copticum. Bangladesh Journal of Pharmacology 6: 51–54.

https://doi.org/10.3329/bjp.v6i1.8442

Marhold K. 2011. Apocynaceae. In: Euro+Med Plantbase – the information resource for Euro-Mediterranean plant diversity. Available from http://ww2.bgbm.org/EuroPlusMed [accessed 6 Nov. 2018].

Markgraf F. 1972. Vincetoxicum Wolf. In: Tutin T.G. et al. (eds) Flora Europaea. Vol. 3: 71–73. Cambridge University Press, Cambridge.

Martin E., Doğan G.İ., Erkul S.K. & Eroğlu H.E. 2019. Karyotype analyses of 25 Turkish taxa of Astragalus from the sections Macrophyllium, Hymenostegis, Hymenocoleus, and Anthylloidei (Fabaceae). Turkish Journal of Botany 43 (2): 232–242. https://dergipark.org.tr/tr/pub/tbtkbotany/issue/45555/572487

Morawetz W. 1984. Karyological races and ecology of the Brazilian Duguetia furfuracea as compared with Xylopia aromatica (Annonaceae). Flora 175: 195–209.

Müller K. 2004. PRAP – computation of Bremer support for large datasets. Molecular Phylogenetics and Evolution 31: 780–782. https://doi.org/10.1016/j.ympev.2003.12.006

Müller K. 2005. SeqState: Primer design and sequence statistics for phylogenetic DNA datasets. Applied Bioinformatics 4 (1): 65–69.

Nixon K.C. 1999. The parsimony ratchet, a new method for rapid parsimony analysis. Cladistics 15: 407–414. https://doi.org/10.1006/clad.1999.0121

Nylander J.A.A. 2004. MrModeltest v2. Evolutionary Biology Centre, Uppsala University, Uppsala [program distributed by the author].

Özay S.G. 2013. Bazı Vincetoxicum N.M. Wolf (Asclepiadaceae) türleri üzerinde farmakognozik araştırmalar. PhD thesis, Mersin Üniversitesi, Mersin.

Ozcan M., Ayaz S.H. & İnceer H. 2008. Chromosome counts of some Cirsium (Asteraceae, Cardueae) taxa from Turkey. Caryologia 61 (4): 375–382. https://doi.org/10.1080/00087114.2008.10589649

Pardi P. 1933. Studi sulla cariologia delle Asclepiadaceae. Nuovo Giornale Botanico Italiano 40(4): 576–589.

Pobedimova G. 1952. Antitoxicum. In: Shishkin B.K. & Bobrov E.G. (eds) Flora of USSR. Vol. 18: 674–691. Academy of Science of the USSR Press, Leningrad/Moscow.

Rechinger K.H. 1970. Flora Iranica. Vol. 73. Akademische Druck & Verlagsanstalt, Graz.

Rice A., Glick L., Abadi S., Einhorn M., Kopelman N.M., Salman-Minkov A., Mayzel J., Chay O. & May I. 2015. The chromosome counts database (CCDB) – a community resource of plant chromosome numbers. New Phytologist 206 (1): 19–26. https://doi.org/10.1111/nph.13191

Ronquist F. & Huelsenbeck J.P. 2003. MrBayes version 3.0: bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574. https://doi.org/10.1093/bioinformatics/btg180

Sennblad B. 1997. Phylogeny of the Apocynaceae s.l. PhD thesis, Acta Universitas Uppsaliensis, Uppsala.

Shah S.A. & Ahmad M. 2014. Reassessing palynological characters in the subfamilies Periplocoideae and Asclepiadoideae (Apocynaceae): taxonomic and evolutionary implications. Palynology 38 (2): 224–234. https://doi.org/10.1080/01916122.2014.893264

Simmons M.P. & Ochoterena H. 2000. Gaps as characters in sequence-based phylogenetic analyses. Systematic Biology 49: 369–381. https://doi.org/10.1093/sysbio/49.2.369

Stace C.A. 2000. Cytology and cytogenetics as a fundamental taxonomic resource for the 20th and 21st centuries. Taxon 49 (3): 451–477. https://doi.org/10.2307/1224344

Stærk D., Lykkeberg A.K., Christensen J., Budnik B.A., Abe F. & Jaroszewski J.W. 2002. In vitro cytotoxic activity of phenantroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against drug-sensitive and multidrug-resistant cancer cells. Journal of Natural Products 65 (9): 1299–1302. https://doi.org/10.1021/np0106384

Strid A. & Franzen R. 1981. In: Löve A. (ed.) Chromosome number reports LXXIII: 829–842. Taxon 30 (4): 829–861.

Swofford D.L. 2003. PAUP – phylogenetic analysis using parsimony version 4. Sinauer Associates, Sunderland (Massachusetts).

Taberlet P., Gielly L., Pautou G. & Bouvet J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109.

Tsiang Y. & Li P.T. 1977. Asclepiadaceae. In: Tsiang Y. & Li P. (eds) Flora Reiplicae Popularis Sinicae. Vol. 63: 249–575. Science Press, Beijing.

Uhríková A., Feráková V. & Schwarzová T. 1985. Karyologisches studium der Slowakischen Flora VI. Acta Facultatis Rerum Naturalium Universitatis Comenianae-Botanica 32: 57–70.

Van den Brand C., Van Meel F.C.M. & Wieffering J.H. 1979. In: Löve A. (ed.) IOBP chromosome number reports LXIV: 395–397. Taxon 28 (4): 391–408.

Vladimirov V., Coşkunçelebi K. & Tan K. 2015. A new diploid species of Pilosella Asteraceae) from Turkey. Turkish Journal of Botany 39: 70–75. https://doi.org/10.3906/bot-1401-92

White T.J., Bruns T., Lee S. & Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal DNA for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J. & White.T. J. (eds.) PCR Protocols: a Guide to Methods and Applications: 315–321. Academic Press, San Diego (CA).

Woodland D.W. 1997. Contemporary Plant Systematics. Andrews University Press, Berrien Springs (MI).

Yamashiro T., Fukuda T., Yokoyama J. & Maki M. 2004. Molecular phylogeny of Vincetoxicum (Apocynaceae-Asclepiadoideae) based on the nucleotide sequences of cpDNA and nrDNA. Molecular Phylogenetics and Evolution 31 (2): 689–700. https://doi.org/10.1016/j.ympev.2003.08.016

Yamashiro T., Yamashiro A., Yokoyama J. & Maki M. 2008. Morphological aspects and phylogenetic analyses of pollination systems in the Tylophora-Vincetoxicum complex (Apocynaceae-Asclepiadoideae) in Japan. Biological Journal of the Linnean Society 93: 325–341.

https://doi.org/10.1111/j.1095-8312.2007.00896.x

Yaseen S. & Perveen A. 2014. Pollinia morphology to some members of Asclepiadaceae of Pakistan. Bangladesh Journal of Botany 43 (3): 249–253.

Zaidi M.A. & Crow Jr S.A. 2005. Biologically active traditional medicinal herbs from Balochistan, Pakistan. Journal of Ethnopharmacology 96: 331–334.

Zaidi M.A. & Crow Jr S.A. 2012. Cytotxicity of four medicinal plants of Pakistan. Pakistan Journal of Botany 44: 395–397.


Refbacks

  • There are currently no refbacks.


License URL: https://creativecommons.org/licenses/by/4.0/