A new species of Odontophrynus (Anura, Odontophrynidae) from the southern portion of the Mantiqueira mountains
Abstract
Using an integrative approach (morphology of the adult and larvae, bioacoustics, osteology, karyotype, and molecular data), we described a new tetraploid species of Odontophrynus to the Mantiqueira mountain range, in southeastern Brazil. The data suggest that Odontophrynus toledoi sp. nov., O. juquinha and Odontophrynus sp. (aff. juquinha) comprise a clade with specimens distributed along three distinct mountain ranges in Brazil: Mantiqueira (O. toledoi sp. nov.) and Espinhaço (O. juquinha) mountains, both in southeastern Brazil, and Diamantina Plateau (O. aff. juquinha), in northeastern Brazil. The new species is morphologically similar and closely related to O. juquinha, but is distinguished in morphology (both adult and larval), karyotype (O. toledoi sp. nov. is tetraploid and O. juquinha is diploid), and corroborated by phylogenetic inferences. We also show that these species do not exchange haplotypes in the 16s gene. Furthermore, although the raw acoustic parameters of Odontophrynus toledoi sp. nov. and O. juquinha overlap at the limits of their ranges, we found a clear difference in the acoustic space structure.
References
Altig R. & McDiarmid R.W. 1999. Body plan, development and morphology. In: McDiarmid R.W. & Altig R. (eds) Tadpoles, the Biology of Anuran Larvae: 24–51. University of Chicago Press, Chicago, IL.
Andrade F.S. de, Haga I.A., Ferreira J.S., Recco-Pimentel S.M., Toledo L.F. & Bruschi D.P. 2020. A new cryptic species of Pithecopus (Anura, Phyllomedusidae) in north-eastern brazil. European Journal of Taxonomy 723: 108–134. https://doi.org/10.5852/ejt.2020.723.1147
Araújo O.G.S., Toledo L.F., Garcia P.C.A. & Haddad C.F.B. 2009. Lista de anfíbios do estado de São Paulo. Biota Neotropica 9 (4): 197–209.
Becak M.L. & Becak W. 1974. Studies on polyploid amphibians: karyotype evolution and phylogeny of the genus Odontophrynus. Journal of Herpetology 8 (4): 337–341. https://doi.org/10.2307/1562903
Beçak M.L., Beçak W. & Rabello M.N. 1966. Cytological evidence of constant tetraploidy in the bisexual South American frog Odontophrynus americanus. Chromosoma 19 (2): 188–193. https://doi.org/10.1007/BF00293683
Blotto B.L., Pereyra M.O., Faivovich J., Dos Santos Dias P.H. & Grant T. 2017. Concentrated evolutionary novelties in the foot musculature of Odontophrynidae (Anura: Neobatrachia), with comments on adaptations for burrowing. Zootaxa 4258 (5): 425–442. https://doi.org/10.11646/zootaxa.4258.5.2
Borteiro C., Kolenc F., Pereyra M.O., Rosset S. & Baldo D. 2010. A diploid surrounded by polyploids: Tadpole description, natural history and cytogenetics of Odontophrynus maisuma Rosset from Uruguay (Anura: Cycloramphidae). Zootaxa 2611 (1): 1–15. https://doi.org/10.11646/zootaxa.2611.1.1
Bouckaert R.R. & Drummond A.J. 2017. bModelTest: Bayesian phylogenetic site model averaging and model comparison. BMC Evolutionary Biology 17 (1): 1–11. https://doi.org/10.1186/s12862-017-0890-6
Bouckaert R., Vaughan T.G., Barido-Sottani J., Duchêne S., Fourment M., Gavryushkina A., Heled J., Jones G., Kühnert D., De Maio N., Matschiner M., Mendes F.K., Müller N.F., Ogilvie H.A., Du Plessis L., Popinga A., Rambaut A., Rasmussen D., Siveroni I., Suchard M.A., Wu C.H., Xie D., Zhang C., Stadler T. & Drummond A.J. 2019. BEAST 2.5: an advanced software platform for Bayesian evolutionary analysis. PLoS Computational Biology 15 (4): e1006650. https://doi.org/10.1371/journal.pcbi.1006650
Caramaschi U. 1979. O girino de Odontophrynus carvalhoi Savage & Cei, 1965 (Amphibia, Anura, Ceratophrydidae). Revista Brasileira de Biologia 39 (1): 169–171.
Caramaschi U. & Napoli M.F. 2012. Taxonomic revision of the Odontophrynus cultripes species group, with description of a new related species (Anura, Cycloramphidae). Zootaxa 3155 (1): 1–20. https://doi.org/10.11646/zootaxa.3155.1.1
Cei J.M. 1980. Amphibians of Argentina. Vol. 2. Universitá degli studi di Firenze.
Cei J. 1985. Un nuevo y peculiar Odontophrynus de la sierra de Guasayán, Santiago del Estero, Argentina (Anura: Leptodactylidae). Cuadernos de Herpetología 1 (5): 1–13.
Cei J.M. 1987. Additional notes to Amphibians of Argentina: an update, 1980–1986. Monitore Zoologico Italiano 21 (3): 209–272.
Cianciarullo A.M., Bonini-Domingos C.R., Vizotto L.D., Kobashi L.S., Beçak M.L. & Beçak W. 2019. Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura). Genetics and Molecular Biology 42 (2): 436–444. https://doi.org/10.1590/1678-4685-gmb-2017-0260
Colombo A.F. & Joly C.A. 2010. Brazilian Atlantic Forest lato sensu: The most ancient Brazilian forest, and a biodiversity hotspot, is highly threatened by climate change. Brazilian Journal of Biology 70 (3): 697–708. https://doi.org/10.1590/s1519-69842010000400002
Cruz C.A.G. & Feio R.N. 2007. Endemismos em anfíbios em áreas de altitude na Mata Atlântica no sudeste do Brasil. In: Nascimento L.B. & Oliveira M.E. (eds) Herpetologia no Brasil II: 117–126. Sociedade Brasileira de Herpetologia, Belo Horizonte, MG.
Drummond A.J. & Rambaut A. 2007. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7 (1): 1–8. https://doi.org/10.1186/1471-2148-7-214
Drummond A.J., Suchard M.A., Xie D. & Rambaut A. 2012. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution 29 (8): 1969–1973. https://doi.org/10.1093/molbev/mss075
Duellman W. & Trueb L. 1994. Biology of Amphibians. The Johns Hopkins University Press, Baltimore, MD.
Duméril A.M.C. & Bibron G. 1841. Erpétologie Genérale ou Histoire Naturelle Complète des Reptiles. Vol. 8. Roret, Paris. https://doi.org/10.5962/bhl.title.45973
Edgar R.C. 2004. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32 (5): 1792–1797. https://doi.org/10.1093/nar/gkh340
Fabrezi M. & Vera R. 1997. Caracterización morfológica de larvas de anuros del noroeste argentino. Cuadernos de herpetología 11 (1–2): 37–49.
Fernández K. & Fernández M. 1921. Sobre la biología y reproducción de algunos batracios argentinos. I. Cystignathidae. Anales de la Sociedad Científica Argentina 91: 97–140.
Frost D.R. 2021. Amphibian Species of the World: an Online Reference. Version 6.1. Available from https://amphibiansoftheworld.amnh.org/ [accessed 24 Oct. 2022].
Gatto K.P., Mattos J. V., Senger K.R. & Lourenço L.B. 2018. Sex chromosome differentiation in the frog genus Pseudis involves satellite DNA and chromosome rearrangements. Frontiers in Genetics 9 (301): 1–12. https://doi.org/10.3389/fgene.2018.00301
González E., Galvani G., Sanabria E., Barrasso D., Alcalde L. & Quiroga L. 2014. The tadpole of Odontophrynus barrioi Cei, Ruiz, and Beçak, 1982 (Anura: Odontophrynidae): a comparison with the other tadpoles of the genus. Acta Herpetologica 9 (1): 15–23. https://doi.org/10.13128/Acta_Herpetol-12931
Gosner K.L. 1960. A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 16 (3): 183–190.
Grenat P.R., Zavala Gallo L.M., Salas N.E. & Martino A.L. 2009. The tadpole of Odontophrynus cordobae Martino & Sinsch, 2002 (Anura: Cycloramphidae) from central Argentina. Zootaxa 2151 (1): 66–68.
Grosjean S. 2005. The choice of external morphological characters and developmental stages for tadpole-based anuran taxonomy: a case study in Rana (Sylvirana) nigrovittata (Blyth, 1855) (Amphibia, Anura, Ranidae). Contributions to Zoology 74 (1–2): 61–76. https://doi.org/10.1163/18759866-0740102005
Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W. & Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology 59 (3): 307–321. https://doi.org/10.1093/sysbio/syq010
Heyer W.R., Rand A.S., Cruz C.A.G. da, Peixoto O.L. & Nelson C. 1990. Frogs of Boracéia. Arquivos de Zoologia 31 (4): 231–410.
Hoang D.T., Vinh L.S., Flouri T., Stamatakis A., Von Haeseler A. & Minh B.Q. 2018. MPBoot: Fast phylogenetic maximum parsimony tree inference and bootstrap approximation. BMC Evolutionary Biology 18 (1): 1–11. https://doi.org/10.1186/s12862-018-1131-3
Hoffman E.A. & Blouin M.S. 2000. A review of colour and pattern polymorphisms in anurans. Biological Journal of the Linnean Society 70 (4): 633–665. https://doi.org/10.1006/bijl.1999.0421
Holloway A.K., Cannatella D.C., Gerhardt H.C. & Hillis D.M. 2006. Polyploids with different origins and ancestors form a single sexual polyploid species. The American Naturalist 167 (4): E88–E101. https://doi.org/10.1086/501079
Howell W.M. & Black D.A. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1016. https://doi.org/10.1007/BF01953855
Josse J. & Husson F. 2016. missMDA: a package for handling missing values in multivariate data analysis. Journal of Statistical Software 70 (1): 1–31. https://doi.org/10.18637/jss.v070.i01
Juncá F.A., Funch L. & Rocha W. 2005. Biodiversidade e Conservação da Chapada Diamantina. First Edition. Ministério do Meio Ambiente, Brasília.
Kalyaanamoorthy S., Minh B.Q., Wong T.K.F., Von Haeseler A. & Jermiin L.S. 2017. ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14 (6): 587–589. https://doi.org/10.1038/nmeth.4285
King M. & Rofe R. 1976. Karyotypic variation in the Australian gekko Phyllodactylus mamoratus (Gray) (Gekkonidae: Reptilia). Chromosoma 54: 75–87.
King M. 1980. C-banding studies in Australian hylid frogs: secondary constriction structure and the concept of euchromatin transformation. Chromosoma 80: 191–207. https://doi.org/10.1007/BF00331835
Köhler J., Jansen M., Rodríguez A., Kok P.J.R., Toledo L.F., Emmrich M., Glaw F., Haddad C.F.B., Rödel M.O. & Vences M. 2017. The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa 4251 (1): 1–124. https://doi.org/10.11646/zootaxa.4251.1.1
Lannoo M.J. 1987. Neuromast topography in tadpoles. Journal of Morphology 191: 247–263. https://doi.org/10.1002/jmor.1051910203
Lavilla E.O. & Scrocchi G.J. 1986. Morfometría larval de los géneros de Telmatobiinae (Anura: Leptodactylidae) decArgentina y Chile. Physis 44: 39–43.
Lavilla E. O. & Scrocchi G. J. 1991. Aportes a la herpetofauna del Chaco argentino: II- Nuevos datos sobre Odontophrynus lavillai Cei, 1985 (Anura: Leptodactylidae). Acta Zoologica Lilloana 40: 33–37.
Leigh J.W. & Bryant D. 2015. POPART: full-feature software for haplotype network construction. Methods in Ecology and Evolution 6 (9): 1110–1116. https://doi.org/10.1111/2041-210X.12410
Lynch J.D. 1971. Evolutionary Relationships, Osteology, and Zoogeography of Leptodactyloid Frogs. University of Kansas Publications, Museum of Natural History, Lawrence, Kansas. Available from https://www.biodiversitylibrary.org/page/3662221 [accessed 24 Oct. 2022].
Lyra M.L, Haddad C.F.B. & Azeredo-Espin A.M.L. 2017. Meeting the challenge of DNA barcoding Neotropical amphibians: polymerase chain reaction optimization and new COI primers. Molecular Ecology Resources 17 (5): 966–980. https://doi.org/10.1111/1755-0998.12648
MacLeod N., Krieger J. & Jones K.E. 2013. Geometric morphometric approaches to acoustic signal analysis in mammalian biology. Hystrix 24 (1): 110–125. https://doi.org/10.4404/hystrix-24.1-6299
Magalhães F. de M., Brandão R.A., Garda A.A. & Mângia S. 2020. Revisiting the generic position and acoustic diagnosis of Odontophrynus salvatori (Anura: Odontophrynidae). Herpetological Journal 30 (4): 189–196. https://doi.org/10.33256/hj30.4.189196
Martino A.L., Dehling J.M. & Sinsch U. 2019. Integrative taxonomic reassessment of Odontophrynus populations in Argentina and phylogenetic relationships within Odontophrynidae (Anura). PeerJ 7: e6480. https://doi.org/10.7717/peerj.6480
Martino A.L. & Sinsch U. 2002. Speciation by polyploidy in Odontophrynus americanus. Journal of Zoology 257 (1): 67–81. https://doi.org/10.1017/S0952836902000663
Martins I.A. & Jim J. 2003. Bioacoustic analysis of advertisement call in Hyla nana and Hyla sanborni (Anura, Hylidae) in Botucatu, São Paulo, Brazil. Revista Brasileira de Biologia 63 (3): 507–516. https://doi.org/10.1590/S1519-69842003000300017
Menegucci R.C., Santos M.T.T., Magalhães R.F. de, Machado I.F., Garcia P.C.A. & Pezzuti T.L. 2016. The tadpole of Odontophrynus monachus Caramaschi & Napoli, 2012 (Amphibia, Anura: Odontophrynidae). Zootaxa 4161 (4): 549–553. https://doi.org/10.11646/zootaxa.4161.4.5
Muscat E., Stuginski D., Nunes I., Martins I., Augusto-Alves G, Vittorazzi S.E., Toledo L.F. & Moroti M.T. 2020. Update on the geographic distribution of three poorly known frog species in the Mantiqueira mountain range. Herpetology Notes 13: 573–577.
Nascimento F.A.C., Mott T., Langone J.A., Davis C.A. & Sá R.O. 2013. The genus Odontophrynus (Anura: Odontophrynidae): a larval perspective. Zootaxa 3700 (1): 140–158. https://doi.org/10.11646/zootaxa.3700.L5
Nguyen L.T., Schmidt H.A., Von Haeseler A. & Minh B.Q. 2015. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
Padial J.M., Miralles A., De la Riva I. & Vences M. 2010. The integrative future of taxonomy review. Frontiers in Zoology 7: 16. https://doi.org/10.1186/1742-9994-7-16
Peixoto M.A., Guedes T.B., Silva E.T. da, Feio R.N. & Romano P.S.R. 2020. Biogeographic tools help to assess the effectiveness of protected areas for the conservation of anurans in the Mantiqueira mountain range, Southeastern Brazil. Journal for Nature Conservation 54: 125799. https://doi.org/10.1016/j.jnc.2020.125799
Pinheiro P.D.P., Pezzuti T.L. & Garcia P.C.A. 2012. The tadpole and vocalizations of Hypsiboas polytaenius (Cope, 1870) (Anura, Hylidae, Hylinae). South American Journal of Herpetology 7 (2): 123–133. https://doi.org/10.2994/057.007.0202
Pons J., Barraclough T.G., Gomez-Zurita J., Cardoso A., Duran D.P., Hazell S., Kamoun S., Sumlin W.D. & Vogler A.P. 2006. Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Systematic Biology 55 (4): 595–609. https://doi.org/10.1080/10635150600852011
Pyron A.R. & Wiens J.J. 2011. A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians. Molecular Phylogenetics and Evolution 61 (2): 543–583. https://doi.org/10.1016/j.ympev.2011.06.012
R Core Team 2022. R: A language and environment for statistical computing, ver. 4.1.3. R Foundation for Statistical Computing, Vienna, Austria. Available from https://www.R-project.org/ [accessed 24 Oct. 2022].
Rannala B. & Yang Z. 2003. Bayes estimation of species divergence times and ancestral population sizes using DNA sequences from multiple loci. Genetics 164 (4): 1645–1656. https://doi.org/10.1093/genetics/164.4.1645
Reid N.M. & Carstens B.C. 2012. Phylogenetic estimation error can decrease the accuracy of species delimitation: a Bayesian implementation of the general mixed Yule-coalescent model. BMC Evolutionary Biology 12: 196. https://doi.org/10.1186/1471-2148-12-196
Roberts J.L., Brown J.L., May R. von, Arizabal W., Schulte R. & Summers K. 2006. Genetic divergence and speciation in lowland and montane peruvian poison frogs. Molecular Phylogenetics and Evolution 41 (1): 149–164. https://doi.org/10.1016/j.ympev.2006.05.005
Rocha P.C. & Romano P.S.R. 2021. The shape of sound: a new R package that crosses the bridge between bioacoustics and geometric morphometrics. Methods in Ecology and Evolution 12 (6): 1115–1121. https://doi.org/10.1111/2041-210X.13580
Rocha P.C., De Sena L.M.F., Pezzuti T.L., Leite F.S.F., Svartman M., Rosset S.D., Baldo D. & Garcia P.C. de A. 2017. A new diploid species belonging to the Odontophrynus americanus species group (Anura: Odontophrynidae) from the espinhaço range, Brazil. Zootaxa 4329 (4): 327–350. https://doi.org/10.11646/zootaxa.4329.4.2
Rohlf F.J. 2015. The tps series of software. Hystrix 26 (1): 1–4. https://doi.org/10.4404/hystrix-26.1-11264
Rohlf F.J. 2017. tpsDig2, Digitize Landmarks and Outlines. Version 2.31. Department of Ecology and Evolution, State University of New York at Stony Brook.
Rossa-Feres D.D.C., Sawaya R.J., Brasileiro C.A., Schiesari L., Nazareth J., Gallardo R.A., Aires B., Vista B., Claro R. & Artes E. De 2010. Anfíbios do Estado de São Paulo, Brasil: conhecimento atual e perspectivas. Biota Neotropica 11: 47–66.
Rosset S.D. 2008. New species of Odontophrynus Reinhardt and Lütken 1862 (Anura: Neobatrachia) from Brazil and Uruguay. Journal of Herpetology 42 (1): 134–144. https://doi.org/10.1670/07-088R1.1
Rosset S.D. & Baldo J.D. 2014. The advertisement call and geographic distribution of Odontophrynus lavillai Cei, 1985 (Anura: Odontophrynidae). Zootaxa 3784 (1): 79–83. https://doi.org/10.11646/zootaxa.3784.1.5
Rosset S.D., Baldo D. & Haddad C.F.B. 2009. Amphibia, Anura, Cycloramphidae, Odontophrynus lavillai: first record for Brazil and geographic distribution map. Check List 5 (1): 32–34. https://doi.org/10.15560/5.1.32
Rosset S.D., Baldo D., Lanzone C. & Basso N.G. 2006. Review of the geographic distribution of diploid and tetraploid populations of the Odontophrynus americanus species complex (Anura: Leptodactylidae). Journal of Herpetology 40 (4): 465–477. https://doi.org/dqq23p
Rosset S.D., Fadel R.M., da Silva Guimarães C., Carvalho P.S., Ceron K., Pedrozo M., Serejo R., dos Santos Souza V., Baldo D. & Mângia S. 2021. A new burrowing frog of the Odontophrynus americanus species group (Anura, Odontophrynidae) from subtropical regions of Argentina, Brazil, and Paraguay. Ichthyology and Herpetology 109 (1): 228–244. https://doi.org/10.1643/h2020056
Saez F.A. & Brum Zorrilla N. 1966. Karyotype variation in some species of the genus Odontophrynus (Amphibia-Anura). Caryologia 19 (1): 55–63. https://doi.org/10.1080/00087114.1966.10796204
Salas N. & Martino A. 2007. Karyotype of Odontophrynus cordobae Martino & Sinsch , 2002 (Anura, Leptodactylidae). Journal of Basic and Applied Genetics 18 (1): 1–5.
Santana D.J., da Silva L.A., Sant’Anna A.C., Shepard D.B. & Mângia S. 2021. A new species of Proceratophrys Miranda- Ribeiro, 1920 (Anura, Odontophrynidae) from Southern Amazonia, Brazil. PeerJ 9: e12012. https://doi.org/10.7717/peerj.12012
Santos D.L., Andrade S.P., Rocha C.F., Maciel N.M., Caramaschi U. & Vaz-Silva W. 2017. Redescription of the tadpole of Odontophrynus carvalhoi Savage and Cei, 1965 (Anura, Odontophrynidae) with comments on the geographical distribution of the species. Zootaxa 4323 (3): 419–422. https://doi.org/10.11646/zootaxa.4323.3.7
Savage J.M. & Cei J.M.A.M. 1965. A review of the leptodactylid frog genus, Odontophrynus. Herpetologica 21 (3): 178–195.
Savage J.M. & Heyer W.R. 1997. Digital webbing formulae for Anurans: a refinement. Herpetological Review 28 (3): 131.
Schmid M., Olert J. & Klett C. 1979. Chromosome banding in amphibia - III. Sex chromosomes in Triturus. Chromosoma 71 (1): 29–55. https://doi.org/10.1007/BF00426365
Silva E.T. da, Peixoto M.A.A., Leite F.S.F., Feio R.N. & Garcia P.C.A. 2018. Anuran distribution in a highly diverse region of the Atlantic Forest: the Mantiqueira Mountain Range in Southeastern Brazil. Herpetologica 74 (4): 294–305. https://doi.org/10.1655/0018-0831.294
Stöck M. 1998. Mating call differences between diploid and tetraploid green toads (Bufo viridis complex) in Middle Asia. Amphibia Reptilia 19 (1): 29–42. https://doi.org/10.1163/156853898X00313
Talavera G. & Castresana J. 2007. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56 (4): 564–577. https://doi.org/10.1080/10635150701472164
Trueb L. 1977. Osteology and anuran systematics: intrapopulational variation in Hyla lanciformis. Systematic Biology 26 (2): 165–184. https://doi.org/10.1093/sysbio/26.2.165
Van Buskirk J. & McCollum S. A. 1999. Plasticity and selection explain variation in tadpole phenotype between ponds with different predator composition. Oikos 85: 31–39. https://doi.org/10.2307/3546789
Van Buskirk J., McCollum S.A. & Werner E.E. 1997. Natural selection for environmentally induced phenotypes in tadpoles. Evolution 51 (6): 1983–1992. https://doi.org/10.1111/j.1558-5646.1997.tb05119.x
Vijayakumar S.P., Menezes R.C., Jayarajan A. & Shanker K. 2016. Glaciations, gradients, and geography: Multiple drivers of diversification of bush frogs in the western ghats escarpment. Proceedings of the Royal Society B: Biological Sciences 283 (1836): 28320161011. https://doi.org/10.1098/rspb.2016.1011
Wasserman A.O. 1970. Polyploidy in the common tree toad Hyla versicolor Le Conte. Science 167: 385–386. https://doi.org/10.1126/science.167.3917.385
Watters J.L., Cummings S.T., Flanagan R.L. & Siler C.D. 2016. Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approach. Zootaxa 4072 (4): 477–495. https://doi.org/10.11646/zootaxa.4072.4.6
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