A DNA barcode-based survey of terrestrial arthropods in the Society Islands of French Polynesia: host diversity within the SymbioCode Project

Thibault Ramage, Patricia Martins-Simoes, Gladys Mialdea, Roland Allemand, Anne Duplouy, Pascal Rousse, Neil Davies, George K. Roderick, Sylvain Charlat


We report here on the taxonomic and molecular diversity of 10 929 terrestrial arthropod specimens, collected on four islands of the Society Archipelago, French Polynesia. The survey was part of the ‘SymbioCode Project’ that aims to establish the Society Islands as a natural laboratory in which to investigate the flux of bacterial symbionts (e.g., Wolbachia) and other genetic material among branches of the arthropod tree. The sample includes an estimated 1127 species, of which 1098 included at least one DNA-barcoded specimen and 29 were identified to species level using morphological traits only. Species counts based on molecular data emphasize that some groups have been understudied in this region and deserve more focused taxonomic effort, notably Diptera, Lepidoptera and Hymenoptera. Some taxa that were also subjected to morphological scrutiny reveal a consistent match between DNA and morphology-based species boundaries in 90% of the cases, with a larger than expected genetic diversity in the remaining 10%. Many species from this sample are new to this region or are undescribed. Some are under description, but many await inspection by motivated experts, who can use the online images or request access to ethanol-stored specimens.


arthropods; DNA barcoding; French Polynesia; Moorea BioCode; SymbioCode

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DOI: https://doi.org/10.5852/ejt.2017.272


Anisimova M. & Gascuel O. 2006. Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Systematic Biology 55: 539–552.

Bailly-Bechet M., Simoes P., Szöllősi G., Mialdea G., Sagot M.-F. & Charlat S. In press. How long does Wolbachia remain on board? Molecular Biology and Evolution.

Check E. 2006. Treasure island: pinning down a model ecosystem. Nature 439: 378–379. http://dx.doi.org/10.1038/439378a

Dierkens M. & Charlat S. 2009. Contribution à la connaissance des araignées des îles de la Société (Polynésie française). Revue Arachnologique 17: 63–81.

Dierkens M. & Ramage T. 2016. Deuxième contribution à la connaissance des araignées de Polynésie française. Bulletin mensuel de la Société Linnéenne de Lyon 85: 134–172.

Fairmaire L.M.H. 1849. Essai sur les Coléoptères de la Polynésie. Revue et Magasin de Zoologie 2: 277–291, 352–365, 410–422, 445–460, 504–516, 550–559.

Fairmaire L.M.H. 1850. Essai sur les Coléoptères de la Polynésie. Revue et Magasin de Zoologie 2: 50–64, 115–122, 181–185.

Folmer O., Black M., Hoeh W., Lutz R. & Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299.

Gillespie R.G. & Roderick G.K. 2002. Arthropods on islands: colonization, speciation, and conservation. Annual Review of Entomology 47: 595–632.

Gressitt J.L. 1956. Some distribtion patterns of Pacific island faunae. Systematic Zoology 5: 11–47.

Gressitt J.L. & Gressitt M.K. 1962. An improved Malaise trap. Pacific Insects 4: 87–90.

Han M.V. & Zmasek C.M. 2009. phyloXML: XML for evolutionary biology and comparative genomics. BMC Bioinformatics 10: e356. http://dx.doi.org/10.1186/1471-2105-10-356

Hurst G.D. & Jiggins F.M. 2005. Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts. Proceedings of the Royal Society B 272: 1525–1534.

Huson D.H. & Scornavacca C. 2012. Dendroscope 3: an interactive tool for rooted phylogenetic trees and networks. Systematic Biology 61: 1061–1067. http://dx.doi.org/10.1093/sysbio/sys062

Katoh K. & Standley D.M. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. http://dx.doi.org/10.1093/molbev/mst010

Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., Buxton S., Cooper A., Markowitz S., Duran C., Thierer T., Ashton B., Meintjes P. & Drummond A. 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649. http://dx.doi.org/10.1093/bioinformatics/bts199

Lamarre G.P.A., Molto Q., Fine P.V.A. & Baraloto C. 2012. A comparison of two common flight interception traps to survey tropical arthropods. ZooKeys 216: 43–55. http://dx.doi.org/10.3897/zookeys.216.3332

Meier R., Shiyang K., Vaidya G. & Ng P.K.L. 2006. DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success. Systematic Biology 55: 715–728. http://dx.doi.org/10.1080/10635150600969864

Miller S. 1996. Biogeography of Pacific insects and other terrestrial invertebrates: a status report. In: Keast A. & Miller S. (eds) The Origin and Evolution of Pacific Island Biotas, New Guinea to Eastern Polynesia: Patterns and Processes: 463–475. Academic Publishers, Amsterdam.

Price M.N., Dehal P.S. & Arkin A.P. 2010. FastTree 2 - Approximately maximum-likelihood trees for large alignments. PLoS ONE 5 (3): e9490. http://dx.doi.org/10.1371/journal.pone.0009490

Ramage T. In press. Checklist of the terrestrial and freshwater arthropods of French Polynesia (Chelicerata; Myriapoda; Crustacea; Hexapoda). Zoosystema.

Ramage T. & Gourvès J. 2016. Un nouveau Scutelleridae pour la Polynésie française, Calliphara bifasciata White, 1839 (Hemiptera). Bulletin de la Société Entomologique de France, in press.

Ramage T. & Kimsey L.S. 2015. The Aculeata of French Polynesia. IV. First record of Chrysis angolensis (Hymenoptera, Chrysididae). Bulletin de la Société Entomologique de France 120: 209–211.

Ramage T., Charlat S. & Jacq F. 2015. The Aculeata of French Polynesia. III. Sphecidae, with the record of three new species for the Society Islands (Hymenoptera). Bulletin de la Société Entomologique de France 120: 157–163.

Ratnasingham S. & Hebert P.D.N. 2013. A DNA-based registry for all animal species: the Barcode Index Number (BIN) system. PLoS ONE 8 (7): e66213. http://dx.doi.org/10.1371/journal.pone.0066213

Rousse O., Ramage T. & Charlat S. In prep. Ichneumonid wasps of French Polynesia: synopsis, illustrated key, and description of 10 new species.

Whittaker R. & Fernández-Palacios J. 2009. Island Biogeography. Ecology, Evolution, and Conservation. Second Edition. Oxford University Press, Oxford.

Wilson J.J., Rougerie R., Schonfeld J., Janzen D.H., Hallwachs W., Hajibabaei M., Kitching I.J., Haxaire J. & Hebert P.D. 2011. When species matches are unavailable are DNA barcodes correctly assigned to higher taxa? An assessment using sphingid moths. BMC Ecology 11: e18. http://dx.doi.org/10.1186/1472-6785-11-18


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